##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547148_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5110745
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97647701069022	31.0	31.0	33.0	30.0	34.0
2	31.348786331542662	31.0	31.0	34.0	30.0	34.0
3	31.52129131858467	31.0	31.0	34.0	30.0	34.0
4	35.296303963512166	37.0	35.0	37.0	33.0	37.0
5	35.08082833324691	37.0	35.0	37.0	32.0	37.0
6	35.154548700825416	37.0	35.0	37.0	32.0	37.0
7	35.656977407403424	37.0	35.0	37.0	35.0	37.0
8	35.69607053374801	37.0	35.0	37.0	35.0	37.0
9	37.485043961301145	39.0	37.0	39.0	35.0	39.0
10	36.69381567657944	39.0	37.0	39.0	32.0	39.0
11	36.32988908662044	38.0	35.0	39.0	32.0	39.0
12	35.8636826920537	37.0	35.0	39.0	31.0	39.0
13	35.72611253349561	37.0	35.0	39.0	30.0	39.0
14	36.67720733474278	38.0	35.0	40.0	31.0	41.0
15	36.85384929203081	38.0	35.0	41.0	31.0	41.0
16	36.89621356573259	38.0	35.0	41.0	31.0	41.0
17	36.78431911590189	38.0	35.0	40.0	31.0	41.0
18	36.7031415185066	38.0	35.0	40.0	31.0	41.0
19	36.70618588875008	38.0	35.0	40.0	31.0	41.0
20	36.57085669506109	38.0	35.0	40.0	31.0	41.0
21	36.45371741301904	38.0	35.0	40.0	30.0	41.0
22	36.404008417559474	38.0	35.0	40.0	30.0	41.0
23	36.2011739971374	38.0	34.0	40.0	30.0	41.0
24	36.19740644465728	38.0	34.0	40.0	30.0	41.0
25	36.0511113350402	38.0	34.0	40.0	30.0	41.0
26	35.928604733752124	38.0	34.0	40.0	30.0	41.0
27	35.74583862039683	38.0	34.0	40.0	29.0	41.0
28	35.69478696354445	38.0	34.0	40.0	29.0	41.0
29	35.56618927377515	38.0	34.0	40.0	29.0	41.0
30	35.56451261019675	38.0	34.0	40.0	29.0	41.0
31	35.52544472479061	38.0	34.0	40.0	28.0	41.0
32	35.3633564186826	38.0	34.0	40.0	27.0	41.0
33	35.20559350936116	38.0	34.0	40.0	27.0	41.0
34	35.1135673957515	38.0	34.0	40.0	26.0	41.0
35	34.93881576952088	38.0	33.0	40.0	25.0	41.0
36	34.77135955716828	37.0	33.0	40.0	24.0	41.0
37	34.88511987978269	38.0	33.0	40.0	25.0	41.0
38	34.84687203137703	38.0	33.0	40.0	25.0	41.0
39	34.770818344487935	38.0	33.0	40.0	24.0	41.0
40	34.77305617869802	38.0	33.0	40.0	24.0	41.0
41	34.796434375027516	37.0	33.0	40.0	24.0	41.0
42	34.57017205906379	37.0	33.0	40.0	24.0	41.0
43	34.633142917519855	37.0	33.0	40.0	24.0	41.0
44	34.45094110545527	37.0	33.0	40.0	23.0	41.0
45	34.194734427172556	37.0	33.0	40.0	23.0	41.0
46	34.30463953885393	37.0	33.0	40.0	23.0	41.0
47	34.1795025969795	37.0	33.0	40.0	23.0	41.0
48	34.25687976997483	37.0	33.0	40.0	23.0	41.0
49	34.179406720546616	36.0	33.0	40.0	24.0	41.0
50	33.69588210720746	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	6.0
13	22.0
14	56.0
15	128.0
16	455.0
17	1188.0
18	2596.0
19	5289.0
20	9501.0
21	15006.0
22	23168.0
23	32438.0
24	45894.0
25	63236.0
26	82433.0
27	100756.0
28	115271.0
29	134250.0
30	159987.0
31	193201.0
32	234248.0
33	291761.0
34	417137.0
35	511951.0
36	427642.0
37	524614.0
38	724402.0
39	994104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.95385604251435	23.3528966911869	29.050383065482627	13.642864200816124
2	31.24746000827668	25.585369647673676	29.674284277536838	13.492886066512808
3	29.35055456689778	25.705039089213024	31.0978732063525	13.8465331375367
4	25.881021260109826	27.48530791499087	31.404501692023373	15.229169132875931
5	23.21238880045864	31.939687853727783	30.234222212221507	14.61370113359207
6	20.915287301557797	41.555350540870265	27.37150845913854	10.1578536984334
7	88.2592263945863	3.7116702163774558	6.481990394746755	1.5471129942894823
8	89.06916701968109	3.1098401505064333	6.077411414578501	1.7435814152339826
9	83.94079532436074	5.353368246703758	7.727366558104542	2.9784698708309647
10	39.87330614225519	32.03129876368318	15.977944507111976	12.117450586949651
11	31.903685274847405	25.044978765326775	26.647954456737715	16.403381503088102
12	30.114650603776944	23.719438164103277	28.593972111698	17.571939120421778
13	22.93227308347413	28.616551989974067	30.0643448264392	18.386830100112604
14	19.00000880497853	32.57495727139585	30.06864948260968	18.356384441015937
15	18.387847564298358	26.93898834710008	37.60614157035814	17.067022518243427
16	21.86442485391073	25.104324320622535	35.879778779806074	17.15147204566066
17	21.572099566697222	25.059223263927276	29.564222045905247	23.80445512347026
18	22.451893021467516	26.790555975694346	32.41302393290998	18.34452706992816
19	26.05858832714213	27.344839157500523	28.246253726217997	18.35031878913935
20	28.28186497271924	26.682333006244686	27.907672951790786	17.128129069245286
21	24.694677586144486	27.68068060527379	30.000146749642177	17.624495058939548
22	24.047550797388638	24.986161508742853	29.355583187969657	21.610704505898845
23	21.832961730628313	28.226530574309617	29.73877585361821	20.20173184144386
24	20.758030385002577	26.326494473897643	33.75006970608003	19.165405435019746
25	20.697843465091683	28.007658374659663	31.80414988421453	19.49034827603412
26	19.907841224713813	31.624547888810735	28.98919824800494	19.478412638470516
27	20.11767364640576	31.034457794313745	30.129110335185967	18.718758224094532
28	18.776225383970438	29.281738768026973	33.240907147588075	18.701128700414518
29	20.09965279034661	27.345621822258792	33.09133991228285	19.46338547511175
30	20.953990073854204	29.293615705733707	30.73749521840749	19.0148990020046
31	24.47198989579797	27.917162761984798	28.637918737874813	18.97292860434242
32	25.590672201410946	27.724431565261032	28.85549954067362	17.8293966926544
33	25.15885648765493	27.863511092805453	28.128364847003716	18.8492675725359
34	21.217885063723585	27.957274330846086	30.011573655112905	20.81326695031742
35	20.76417430335499	27.669547199087415	30.835113080382605	20.731165417174992
36	24.017966069526068	28.754320554048384	28.76459302900066	18.463120347424887
37	21.623559774553417	31.232198045490435	28.659481151965128	18.484761027991027
38	21.382009863532616	31.497188766021395	26.963113988273722	20.157687382172266
39	21.16393989525989	30.324815657991156	27.96116808801848	20.55007635873048
40	23.134533223629823	27.26518345172768	28.650030475009025	20.95025284963347
41	19.73136988834309	27.92847226774179	29.120764193869974	23.21939365004515
42	21.458711009842986	28.099230151377146	27.78804264348935	22.65401619529051
43	21.081623129308934	27.76051241061724	28.47261994092838	22.68524451914545
44	20.789552208141863	28.88066221265197	29.89065194996033	20.439133629245834
45	20.505053568511048	31.3916464233688	27.107730868982898	20.99556913913725
46	20.872847305040654	30.68699377488018	28.371108321780873	20.06905059829829
47	20.92763383811949	28.55086685013633	28.84062891026651	21.68087040147767
48	21.031884783920933	27.726055594634442	30.459512262889266	20.782547358555355
49	21.346065984509107	27.618771822894704	30.390187731925582	20.644974460670607
50	20.461185208810065	30.841707030971023	28.466221656529527	20.230886103689382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3769.0
1	5813.5
2	7858.0
3	23822.5
4	39787.0
5	29585.0
6	19383.0
7	19858.0
8	20333.0
9	21237.5
10	22142.0
11	22395.0
12	22648.0
13	22398.0
14	22148.0
15	21580.5
16	21013.0
17	21685.5
18	22358.0
19	22331.0
20	22304.0
21	24381.0
22	26458.0
23	30807.0
24	35156.0
25	43551.0
26	51946.0
27	65813.5
28	79681.0
29	93791.5
30	107902.0
31	124629.0
32	141356.0
33	167589.0
34	193822.0
35	206890.0
36	219958.0
37	237793.5
38	255629.0
39	276561.0
40	297493.0
41	332188.0
42	366883.0
43	397991.5
44	429100.0
45	432471.5
46	435843.0
47	413754.5
48	391666.0
49	377490.0
50	363314.0
51	328358.0
52	293402.0
53	270308.0
54	247214.0
55	227729.0
56	208244.0
57	196054.0
58	183864.0
59	175337.0
60	166810.0
61	152601.5
62	138393.0
63	116450.0
64	94507.0
65	78766.0
66	63025.0
67	50489.0
68	37953.0
69	30407.0
70	22861.0
71	19025.5
72	15190.0
73	11763.5
74	8337.0
75	6892.5
76	5448.0
77	4064.5
78	2681.0
79	2055.5
80	1430.0
81	1061.5
82	693.0
83	591.5
84	490.0
85	342.5
86	195.0
87	116.5
88	38.0
89	27.0
90	16.0
91	9.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5110745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.28930250829511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06295696059169	14.015219851085762
2	7.273030012252912	2.514912320674997
3	2.264876842206692	1.1747442260683096
4	1.0831764841832805	0.7490946361966507
5	0.6860779304791146	0.5930904442159235
6	0.47999278429329845	0.49792442696674055
7	0.38814180886790484	0.4697490804743847
8	0.2778781930801751	0.3843456112497266
9	0.25425864751413146	0.3956359204999615
>10	3.2156476073145304	13.825106514229876
>50	1.4966227482813144	18.86431799837099
>100	1.5080092901156603	44.87783203576519
>500	0.007833172539811636	0.876073350456623
>1k	0.0013823245658489776	0.37247257889057234
>5k	0.0	0.0
>10k+	1.1519371382074814E-4	0.3894810048542419
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19556	0.3826448003177619	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01577069487912232	0.0
2	0.0	0.0	0.0	0.06591993926521476	0.0
3	0.0	0.0	0.0	0.09521116784343574	0.0
4	0.0	0.0	0.0	0.15766781555330975	0.0
5	0.0	0.0	0.0	0.2890185286098211	0.0
6	0.0	0.0	0.0	0.45848501539403747	0.0
7	0.0	0.0	0.0	0.5578247398373427	0.0
8	0.0	0.0	0.0	0.782429958841617	0.0
9	1.9566618956727443E-5	0.0	0.0	0.8723385729477796	0.0
10	1.9566618956727443E-5	0.0	0.0	1.0435660554381014	0.0
11	1.9566618956727443E-5	0.0	0.0	1.1911766288476533	0.0
12	1.9566618956727443E-5	0.0	0.0	1.3292191255873655	0.0
13	1.9566618956727443E-5	0.0	0.0	1.3835556264301976	0.0
14	1.9566618956727443E-5	0.0	0.0	1.4072703686057513	0.0
15	1.9566618956727443E-5	0.0	0.0	1.4396139897412217	0.0
16	1.9566618956727443E-5	0.0	0.0	1.5069818588092343	0.0
17	3.913323791345489E-5	0.0	0.0	1.5835460387869087	0.0
18	3.913323791345489E-5	0.0	0.0	1.6898319129598522	0.0
19	3.913323791345489E-5	0.0	0.0	1.7551452870374085	0.0
20	3.913323791345489E-5	0.0	0.0	1.8187367986467726	0.0
21	3.913323791345489E-5	0.0	0.0	1.9096433103197283	0.0
22	3.913323791345489E-5	0.0	0.0	2.008161236766851	0.0
23	3.913323791345489E-5	0.0	0.0	2.116051573694246	0.0
24	3.913323791345489E-5	0.0	0.0	2.1981726734556313	0.0
25	3.913323791345489E-5	0.0	0.0	2.2755195181915746	0.0
26	3.913323791345489E-5	0.0	0.0	2.350244435987317	0.0
27	3.913323791345489E-5	0.0	0.0	2.4300958079497215	0.0
28	3.913323791345489E-5	0.0	0.0	2.5166976634521974	0.0
29	3.913323791345489E-5	0.0	0.0	2.612867595624513	0.0
30	5.8699856870182334E-5	0.0	0.0	2.7298172771288725	0.0
31	5.8699856870182334E-5	0.0	0.0	2.836905382679042	0.0
32	5.8699856870182334E-5	0.0	0.0	2.942858624329721	0.0
33	5.8699856870182334E-5	0.0	0.0	3.0530969555319234	0.0
34	5.8699856870182334E-5	0.0	0.0	3.1725707308817013	0.0
35	5.8699856870182334E-5	0.0	0.0	3.2995385212919057	0.0
36	5.8699856870182334E-5	0.0	0.0	3.4189927300227265	0.0
37	7.826647582690977E-5	0.0	0.0	3.5441408248699555	0.0
38	7.826647582690977E-5	0.0	0.0	3.685705313021878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16145	0.0	39.83029	1
TATCGCG	225	0.0	38.13333	1
CGACGGT	770	0.0	37.142853	28
AGGGCGA	3930	0.0	37.114506	6
CGTAAGG	730	0.0	37.068493	2
GGGCGAT	8015	0.0	36.945724	7
CTCACGA	770	0.0	36.85714	24
TGGGCGA	2470	0.0	36.696354	6
TGTAGCG	420	0.0	36.666664	1
TCGATAG	265	0.0	36.5283	1
GACCGAT	2585	0.0	36.51064	9
CGGTCTA	750	0.0	36.08	31
TACGGGA	1855	0.0	35.81671	4
CGACCCG	510	0.0	35.80392	32
GCGCGAC	560	0.0	35.75	9
CACGACG	770	0.0	35.714283	26
TAAGGGA	6435	0.0	35.65812	4
TATAGCG	235	0.0	35.57447	1
TAGGGAC	5400	0.0	35.566666	5
GGACCGA	2820	0.0	35.418438	8
>>END_MODULE