FastQCFastQC Report
Thu 26 May 2016
SRR1547147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547147_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5013297
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196800.3925560364765941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT168500.038.725221
CGGTCTA4750.038.44210431
GGGCGAT68750.037.6327
TATAGCG3850.037.1428571
GACCGAT24350.037.043129
TCACGAC4950.036.8888925
AGGGCGA34150.036.720356
CGAACTA301.3017195E-436.66666830
TAAGGGA55100.036.453724
CGTTAGG7500.036.3733332
TTAGCGG11500.036.3478242
TAGGGAC39850.035.9397745
GGCGATA15500.035.909688
TAGGGCG15050.035.3754165
ATGGGCG15000.035.25
CGTACCG250.00235349735.243
TGGGCGA23050.035.1236426
ATAGCGG10150.035.11332
ATAGGGA64650.034.9822124
CGACGGT5350.034.95327428
Produced by FastQC (version 0.11.5)