##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547147_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5013297
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.993095162724252	31.0	31.0	33.0	30.0	34.0
2	31.380093579135647	31.0	31.0	34.0	30.0	34.0
3	31.53771819223956	31.0	31.0	34.0	30.0	34.0
4	35.310963623340086	37.0	35.0	37.0	33.0	37.0
5	35.09763854006655	37.0	35.0	37.0	32.0	37.0
6	35.158429871599466	37.0	35.0	37.0	32.0	37.0
7	35.65671592965667	37.0	35.0	37.0	35.0	37.0
8	35.695079306093376	37.0	35.0	37.0	35.0	37.0
9	37.47443787990219	39.0	37.0	39.0	35.0	39.0
10	36.72325278155274	39.0	37.0	39.0	32.0	39.0
11	36.38366169010134	38.0	35.0	39.0	32.0	39.0
12	35.85970031298764	37.0	35.0	39.0	31.0	39.0
13	35.682388855078806	37.0	35.0	39.0	30.0	39.0
14	36.599319569536775	38.0	35.0	40.0	31.0	41.0
15	36.8058088319922	38.0	35.0	40.0	31.0	41.0
16	36.87113410595861	38.0	35.0	41.0	31.0	41.0
17	36.77616626343901	38.0	35.0	40.0	31.0	41.0
18	36.69877787013217	38.0	35.0	40.0	31.0	41.0
19	36.6720732882971	38.0	35.0	40.0	31.0	41.0
20	36.5263520194395	38.0	35.0	40.0	30.0	41.0
21	36.42089945997614	38.0	35.0	40.0	30.0	41.0
22	36.35910040837397	38.0	35.0	40.0	30.0	41.0
23	36.181300848523435	38.0	34.0	40.0	30.0	41.0
24	36.140484595267345	38.0	34.0	40.0	30.0	41.0
25	35.99565236210821	38.0	34.0	40.0	30.0	41.0
26	35.88636879083764	38.0	34.0	40.0	30.0	41.0
27	35.70739635014642	38.0	34.0	40.0	29.0	41.0
28	35.64367341492036	38.0	34.0	40.0	29.0	41.0
29	35.53088815603783	38.0	34.0	40.0	29.0	41.0
30	35.51532135439013	38.0	34.0	40.0	28.0	41.0
31	35.46130600281611	38.0	34.0	40.0	27.0	41.0
32	35.306804085215774	38.0	34.0	40.0	27.0	41.0
33	35.13866523367756	38.0	34.0	40.0	26.0	41.0
34	35.052488412316286	38.0	34.0	40.0	26.0	41.0
35	34.86698693494521	37.0	33.0	40.0	25.0	41.0
36	34.66647118652655	37.0	33.0	40.0	24.0	41.0
37	34.76062140343969	37.0	33.0	40.0	24.0	41.0
38	34.71755134395588	37.0	33.0	40.0	24.0	41.0
39	34.62718247093679	37.0	33.0	40.0	24.0	41.0
40	34.6196471104744	37.0	33.0	40.0	24.0	41.0
41	34.648274578585706	37.0	33.0	40.0	24.0	41.0
42	34.41555267920492	37.0	33.0	40.0	23.0	41.0
43	34.479732200186824	37.0	33.0	40.0	24.0	41.0
44	34.28848280881823	37.0	33.0	40.0	23.0	41.0
45	34.034516407067045	37.0	33.0	40.0	23.0	41.0
46	34.150427552965645	37.0	33.0	40.0	23.0	41.0
47	34.02077794313802	36.0	33.0	40.0	23.0	41.0
48	34.0776062140344	36.0	33.0	40.0	23.0	41.0
49	34.00100313227004	36.0	33.0	40.0	23.0	41.0
50	33.531547602306425	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	3.0
12	16.0
13	22.0
14	62.0
15	156.0
16	492.0
17	1313.0
18	2879.0
19	5745.0
20	9880.0
21	15643.0
22	23126.0
23	33298.0
24	46936.0
25	64339.0
26	84820.0
27	102295.0
28	115946.0
29	134037.0
30	157370.0
31	190106.0
32	230200.0
33	285431.0
34	413134.0
35	514537.0
36	411304.0
37	505725.0
38	700118.0
39	964358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.12498740848587	24.11818011181065	28.929285458252323	13.827547021451153
2	31.687290818796495	25.697839166520552	28.950329493744338	13.664540520938614
3	29.478804068460335	26.041265857578356	30.970915946132855	13.509014127828452
4	26.42711971782242	28.02042248843426	30.388285393823665	15.164172399919654
5	23.14660392153108	32.24758078366393	29.549755380540986	15.056059914264006
6	21.32482875042113	40.35633635908665	27.83312059907881	10.485714291413416
7	87.22254037612373	4.1036268148485915	6.850122783469641	1.823710025558031
8	87.63171222451014	3.529593399313865	6.812203625677872	2.0264907504981253
9	82.35835618755482	6.088328698658787	8.547867800371693	3.005447313414705
10	39.72479587784247	31.779784840195983	16.223215979424317	12.272203302537232
11	33.04061179698709	26.21282960095921	25.56225972648339	15.18429887557031
12	30.960024909754996	24.04902402550657	28.332532463167453	16.658418601570983
13	23.23343300825784	30.380845180327437	29.19593632693216	17.189785484482567
14	18.97605507912258	32.80378162315139	30.353677430242016	17.866485867484013
15	18.286528805295198	26.858871517087458	37.969563742184036	16.885035935433308
16	21.121589245560358	25.21456438746797	36.42018416223895	17.243662204732736
17	21.062645999229648	25.45364856700092	29.595314221359715	23.88839121240972
18	23.130327207823516	26.045315089052174	32.46153180232489	18.362825900799415
19	26.48053765815191	26.866491253161346	28.427440065888774	18.225531022797973
20	27.67398380746243	26.748844123936806	28.17008447734096	17.407087591259803
21	24.367556919129267	27.741244933224586	30.36217483225111	17.52902331539504
22	23.601753496750742	25.048386321416828	29.67234935412763	21.6775108277048
23	21.2947088512809	28.536350429667344	29.762748945454458	20.406191773597296
24	20.11169495842756	26.74417653691772	34.612471593045456	18.531656911609264
25	20.623094941313074	27.788977991928267	32.62290265268545	18.965024414073213
26	19.639769995673507	31.63371330284242	29.09370819243304	19.63280850905103
27	19.783627421235966	31.086169440988638	30.46224869581834	18.66795444195706
28	18.034259689780995	29.67538129099473	33.93146266818024	18.35889635104403
29	18.93891385250066	27.69632439490419	33.552390771980996	19.812370980614155
30	20.209155771142225	29.303949077822438	31.2404391760552	19.246455974980137
31	24.304783857808545	28.28234592923579	29.09402734368221	18.318842869273453
32	25.00045379318241	27.480538256560504	29.10918702801769	18.409820922239398
33	24.086923236345264	28.04741071594202	28.246620936282053	19.619045111430662
34	20.418578831455626	28.57307277027473	30.10078995918255	20.907558439087094
35	20.531937365769473	28.565313405529334	30.65758920726221	20.245160021438984
36	24.718363982824076	28.182212224809344	28.065163504176994	19.03426028818959
37	20.74171947123819	31.61069451899618	28.565752238496938	19.081833771268688
38	20.88304363376038	31.751380379020034	27.533916302983847	19.831659684235746
39	21.044594006698585	30.921527290324114	27.80794754430069	20.225931158676616
40	23.309969467198933	27.412977926502258	28.59224179217788	20.684810814120926
41	19.29730075836321	27.778805045861038	28.95475771732654	23.96913647844921
42	20.589743635775022	27.85693726104797	28.269998765283606	23.283320337893407
43	20.55521546000566	27.556276837378675	29.27859650046666	22.609911202149004
44	19.895250570632463	29.399415195229807	30.072963959645715	20.632370274492015
45	19.372261408011536	32.431531584903105	27.327245922194514	20.86896108489084
46	20.744751408105287	31.062871399799374	28.221228465020125	19.971148727075217
47	20.801779746940984	29.154905444461	28.79611959953699	21.247195209061022
48	20.95359600677957	28.926772142165124	30.51778101317357	19.601850837881738
49	21.18775328890349	27.52063163223723	30.8058549094538	20.485760169405484
50	19.729192186299755	30.896653439842087	29.061733226657026	20.312421147201135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4612.0
1	6708.5
2	8805.0
3	24082.0
4	39359.0
5	30020.5
6	20682.0
7	21026.0
8	21370.0
9	22366.5
10	23363.0
11	24001.5
12	24640.0
13	24840.0
14	25040.0
15	25729.5
16	26419.0
17	25342.5
18	24266.0
19	25096.5
20	25927.0
21	30104.0
22	34281.0
23	37916.0
24	41551.0
25	50009.5
26	58468.0
27	69154.0
28	79840.0
29	93194.5
30	106549.0
31	128197.5
32	149846.0
33	163763.5
34	177681.0
35	195912.0
36	214143.0
37	232310.5
38	250478.0
39	271607.5
40	292737.0
41	334349.5
42	375962.0
43	400134.0
44	424306.0
45	429647.5
46	434989.0
47	411602.5
48	388216.0
49	355166.0
50	322116.0
51	304830.5
52	287545.0
53	262015.0
54	236485.0
55	209925.5
56	183366.0
57	177670.0
58	171974.0
59	158816.0
60	145658.0
61	134582.5
62	123507.0
63	109651.0
64	95795.0
65	79759.5
66	63724.0
67	53241.5
68	42759.0
69	35334.5
70	27910.0
71	21775.0
72	15640.0
73	13414.5
74	11189.0
75	9026.0
76	6863.0
77	4594.0
78	2325.0
79	1993.0
80	1661.0
81	1097.5
82	534.0
83	506.5
84	479.0
85	313.0
86	147.0
87	100.5
88	54.0
89	36.0
90	18.0
91	15.0
92	12.0
93	9.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5013297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.444542448027185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.87900197569869	14.283417255671774
2	7.273410412068561	2.5376263335050586
3	2.249805424839163	1.1774047870022584
4	1.144269456546509	0.7984502842682629
5	0.657837390966408	0.5737836145306481
6	0.47188706627871707	0.4939112375024458
7	0.3468645116939422	0.42356248885714387
8	0.26404625350251	0.3684932861973666
9	0.2084157341940665	0.32721454097866304
>10	2.690345195493053	11.565843546224121
>50	1.2336605568313739	15.723851776751225
>100	1.5678962786560198	49.69054478101281
>500	0.011164216205478162	1.244319876399466
>1k	0.0012792331068776988	0.39232917193310884
>5k	0.0	0.0
>10k+	1.1629391880706353E-4	0.39924701916560223
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19680	0.3925560364765941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013464193324273427	0.0
2	0.0	0.0	0.0	0.05724775531950331	0.0
3	0.0	0.0	0.0	0.08708839711670782	0.0
4	0.0	0.0	0.0	0.15365137952130106	0.0
5	0.0	0.0	0.0	0.2679673675826507	0.0
6	0.0	0.0	0.0	0.4643451205863128	0.0
7	0.0	0.0	0.0	0.5686876321111636	0.0
8	0.0	0.0	0.0	0.8167678874800356	0.0
9	0.0	0.0	0.0	0.9130119360572494	0.0
10	0.0	0.0	0.0	1.0673415119830323	0.0
11	0.0	0.0	0.0	1.2200753316629755	0.0
12	0.0	0.0	0.0	1.3494313223413654	0.0
13	0.0	0.0	0.0	1.4067189715670148	0.0
14	0.0	0.0	0.0	1.4327896392334227	0.0
15	0.0	0.0	0.0	1.4651635440708979	0.0
16	0.0	0.0	0.0	1.5317464734285642	0.0
17	0.0	0.0	0.0	1.6072456908098602	0.0
18	0.0	0.0	0.0	1.7079179629692796	0.0
19	1.994695307299767E-5	0.0	0.0	1.7650460365703449	0.0
20	1.994695307299767E-5	0.0	0.0	1.8278589917972146	0.0
21	1.994695307299767E-5	0.0	0.0	1.9157851609429881	0.0
22	1.994695307299767E-5	0.0	0.0	2.0097353099168074	0.0
23	1.994695307299767E-5	0.0	0.0	2.1090312423141895	0.0
24	1.994695307299767E-5	0.0	0.0	2.192868286079999	0.0
25	1.994695307299767E-5	0.0	0.0	2.2656148239372214	0.0
26	1.994695307299767E-5	0.0	0.0	2.3403760040548165	0.0
27	1.994695307299767E-5	0.0	0.0	2.414558722533295	0.0
28	1.994695307299767E-5	0.0	0.0	2.498116508956082	0.0
29	1.994695307299767E-5	0.0	0.0	2.5894735540304117	0.0
30	1.994695307299767E-5	0.0	0.0	2.704966412323068	0.0
31	1.994695307299767E-5	0.0	0.0	2.8045017081573262	0.0
32	1.994695307299767E-5	0.0	0.0	2.902122096496577	0.0
33	1.994695307299767E-5	0.0	0.0	3.0018568618615653	0.0
34	1.994695307299767E-5	0.0	0.0	3.1107073847809135	0.0
35	1.994695307299767E-5	0.0	0.0	3.226818598618833	0.0
36	1.994695307299767E-5	0.0	0.0	3.3350308190398454	0.0
37	1.994695307299767E-5	0.0	0.0	3.4514811310800058	0.0
38	1.994695307299767E-5	0.0	0.0	3.5904914470457268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16850	0.0	38.72522	1
CGGTCTA	475	0.0	38.442104	31
GGGCGAT	6875	0.0	37.632	7
TATAGCG	385	0.0	37.142857	1
GACCGAT	2435	0.0	37.04312	9
TCACGAC	495	0.0	36.88889	25
AGGGCGA	3415	0.0	36.72035	6
CGAACTA	30	1.3017195E-4	36.666668	30
TAAGGGA	5510	0.0	36.45372	4
CGTTAGG	750	0.0	36.373333	2
TTAGCGG	1150	0.0	36.347824	2
TAGGGAC	3985	0.0	35.939774	5
GGCGATA	1550	0.0	35.90968	8
TAGGGCG	1505	0.0	35.375416	5
ATGGGCG	1500	0.0	35.2	5
CGTACCG	25	0.002353497	35.2	43
TGGGCGA	2305	0.0	35.123642	6
ATAGCGG	1015	0.0	35.1133	2
ATAGGGA	6465	0.0	34.982212	4
CGACGGT	535	0.0	34.953274	28
>>END_MODULE