FastQCFastQC Report
Thu 26 May 2016
SRR1547146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547146_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3483166
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137580.39498548159921176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT207.858685E-444.00000416
CGCAATA207.858685E-444.00000440
CGTTTTT123100.040.2469521
TAACGCG404.12776E-738.5000041
GCGATCG1150.038.2608689
CGTAAGG6650.038.045112
TCGAACG357.2944695E-637.7142831
TACGGGA12500.037.3124
CGCATCG651.0913936E-1137.2307721
GACCGAT16600.036.7108429
GCGATAC2500.036.089
TAAGGGA42300.036.0425534
GGGCGAT56300.035.9502647
ATAGGGA47150.035.881234
ACGGGAT15550.035.7942125
AGGGCGA28050.035.7647066
CGGGATT15200.035.6052636
GTAGGGC15450.035.5987054
TAGCACG1300.035.5384641
TAGGGAC29900.035.5384645
Produced by FastQC (version 0.11.5)