FastQCFastQC Report
Thu 26 May 2016
SRR1547145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547145_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2984490
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227990.7639161129707253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC207.8585045E-444.041
CGTTTTT172350.042.2129361
ACGGGTC1100.040.05
GCGTAAG1500.039.61
CGTAAGG5150.039.3009722
GGACGTA1250.038.728
TACGGGA17800.038.3146064
TAGGGCG7700.038.05
TAGGGAC40550.037.9778065
GACGTAC700.037.7142879
CCGCTCG357.2941148E-637.71428719
GGCGATA10400.037.6538438
ACGGGAT24750.037.5111125
TAGGGAT88600.037.3950355
AGGGATT113250.037.3562936
CGTTAGG3850.037.1428572
CGAGGGA25300.037.043484
ACGCCGG2450.036.81632627
GTAGGGA58050.036.799314
AGGGCGA21750.036.717246
Produced by FastQC (version 0.11.5)