##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547145_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2984490
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03971432305017	31.0	31.0	33.0	30.0	34.0
2	31.42247419157042	31.0	31.0	34.0	30.0	34.0
3	31.539153423197934	31.0	31.0	34.0	30.0	34.0
4	35.31794846020593	37.0	35.0	37.0	33.0	37.0
5	35.126262108433934	37.0	35.0	37.0	32.0	37.0
6	35.201413306796134	37.0	35.0	37.0	32.0	37.0
7	35.73530921530982	37.0	35.0	37.0	35.0	37.0
8	35.79137708620233	37.0	35.0	37.0	35.0	37.0
9	37.569677901416995	39.0	37.0	39.0	35.0	39.0
10	36.851394710654084	39.0	37.0	39.0	32.0	39.0
11	36.497167690292144	39.0	35.0	39.0	32.0	39.0
12	36.20482326963736	38.0	35.0	39.0	31.0	39.0
13	36.1224279525145	38.0	35.0	39.0	31.0	39.0
14	37.14293865953647	39.0	36.0	41.0	31.0	41.0
15	37.28507081611934	39.0	36.0	41.0	32.0	41.0
16	37.276260600638636	39.0	35.0	41.0	32.0	41.0
17	37.161095865625285	39.0	35.0	41.0	32.0	41.0
18	37.0261860485376	39.0	36.0	40.0	31.0	41.0
19	36.97932243029798	38.0	35.0	40.0	31.0	41.0
20	36.82342142208552	38.0	35.0	40.0	31.0	41.0
21	36.718301284306534	38.0	35.0	40.0	31.0	41.0
22	36.649709665638014	38.0	35.0	40.0	31.0	41.0
23	36.464109445834964	38.0	35.0	40.0	30.0	41.0
24	36.440115396600426	38.0	35.0	40.0	30.0	41.0
25	36.310396081072476	38.0	35.0	40.0	30.0	41.0
26	36.19996180251902	38.0	35.0	40.0	30.0	41.0
27	36.02867324065418	38.0	34.0	40.0	30.0	41.0
28	35.97495049405426	38.0	34.0	40.0	30.0	41.0
29	35.856116790473415	38.0	34.0	40.0	29.0	41.0
30	35.802335407389535	38.0	34.0	40.0	29.0	41.0
31	35.71247851391695	38.0	34.0	40.0	29.0	41.0
32	35.492151087790546	38.0	34.0	40.0	27.0	41.0
33	35.24146236040328	38.0	34.0	40.0	26.0	41.0
34	35.1141203354677	38.0	34.0	40.0	25.0	41.0
35	34.8969847444622	38.0	34.0	40.0	24.0	41.0
36	34.72197092300527	38.0	33.0	40.0	24.0	41.0
37	34.815525935754515	38.0	34.0	40.0	24.0	41.0
38	34.77013828158245	38.0	33.0	40.0	24.0	41.0
39	34.69728362299757	38.0	33.0	40.0	23.0	41.0
40	34.71390522333799	38.0	33.0	40.0	23.0	41.0
41	34.73326330461821	38.0	34.0	40.0	23.0	41.0
42	34.506774356757774	38.0	33.0	40.0	23.0	41.0
43	34.568201267218186	38.0	33.0	40.0	23.0	41.0
44	34.39025160077601	38.0	33.0	40.0	23.0	41.0
45	34.21798297196506	38.0	33.0	40.0	23.0	41.0
46	34.297886070987005	38.0	33.0	40.0	23.0	41.0
47	34.16045387989238	37.0	33.0	40.0	22.0	41.0
48	34.199505108075414	37.0	33.0	40.0	23.0	41.0
49	34.10786466029372	37.0	33.0	40.0	22.0	41.0
50	33.652441790724716	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	3.0
13	10.0
14	21.0
15	52.0
16	246.0
17	624.0
18	1500.0
19	3127.0
20	5354.0
21	8545.0
22	13145.0
23	18933.0
24	27527.0
25	39533.0
26	53189.0
27	63673.0
28	68506.0
29	75938.0
30	87371.0
31	104948.0
32	126787.0
33	156363.0
34	214496.0
35	270081.0
36	249415.0
37	308647.0
38	441904.0
39	644545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.642190122935578	22.968714922817636	28.031958559083797	17.357136395162993
2	32.36700407774863	24.992109204587717	29.948399894119266	12.69248682354439
3	27.68610382343382	25.52878381230964	33.961916441335035	12.823195922921505
4	24.476476717965216	27.83041658708858	33.62447185281237	14.068634842133832
5	22.213443502910042	31.998364879761702	32.266115818783106	13.522075798545144
6	20.55751568944778	40.39835951871174	29.332549279776444	9.711575512064037
7	87.62351356513173	2.9668385553310617	8.23923015322551	1.1704177263116982
8	88.47303894467731	2.5307506475143158	7.852530918180325	1.1436794896280438
9	82.83599542970491	6.31464672356081	8.956739677465832	1.8926181692684512
10	31.538788871800545	46.56021631836595	14.182892219441179	7.718102590392329
11	23.436298999158986	23.842599573126396	37.322557622910445	15.398543804804172
12	24.696346779516766	24.01344953409125	35.0477971110642	16.242406575327777
13	20.93617334955051	27.391212568981636	35.25309181803256	16.419522263435294
14	16.977976136626356	32.02741506924131	33.88190277065763	17.112706023474697
15	16.65326404176258	29.027136964774552	38.83976826861541	15.479830724847462
16	20.281354603299057	27.74477381395146	36.85467198750875	15.11919959524073
17	20.213771867220196	26.529256254837506	32.55105562424401	20.705916253698287
18	20.667283187412256	27.818019159052298	34.62665313001551	16.888044523519934
19	23.344289979192425	27.610680551786064	31.96552844874669	17.07950102027482
20	25.347345777670554	28.12041588345077	31.240412934873298	15.291825404005374
21	23.40182074659322	27.089921561137746	33.25807759449688	16.250180097772148
22	22.04822264440491	25.057983105991312	33.347037517297764	19.546756732306022
23	19.568603010899686	28.690496533746135	33.372536011177786	18.36836444417639
24	19.053975721144987	27.503057473806248	36.3285519468988	17.114414858149967
25	19.504069371986503	28.92644974518259	33.95652188481114	17.612958998019764
26	18.935496516992853	32.54251145086765	31.06188997115085	17.460102060988643
27	18.495421328267142	31.484240188440904	33.28139816183	16.73894032146196
28	17.559482524652452	29.67716427262279	35.7196706975061	17.043682505218648
29	18.310364584903954	27.55217809407973	35.844784200985764	18.29267312003056
30	19.48061477840435	29.505644180412734	33.407382835928416	17.606358205254498
31	21.83093258814739	28.47260335936793	31.653783393477614	18.042680659007065
32	22.121400976381224	28.81872614751599	31.89054076240832	17.169332113694466
33	21.982047183940974	29.057158844559705	31.46128819329266	17.49950577820666
34	19.82328639063961	29.262353031841286	31.654017939413436	19.260342638105673
35	19.72899892443935	28.834139166155694	32.10123002590058	19.335631883504384
36	22.052779536872297	29.69659807873372	30.24014153171899	18.010480852674995
37	20.157179283562684	31.46222637703594	30.947565580718983	17.433028758682386
38	19.645601090973667	31.308900348133186	29.779861885950364	19.265636674942787
39	19.977282550787574	30.817560119149334	30.431028416915453	18.77412891314764
40	21.52002519693482	28.880780300821918	30.674721644234022	18.924472858009242
41	18.94782693190461	29.37654339602411	30.6161186668409	21.059511005230373
42	20.468120181337515	29.593632412908065	29.252569115661302	20.685678290093115
43	20.14233587648141	29.498942867960693	29.747729092742816	20.610992162815087
44	19.269824995225314	30.98482487795235	30.586063280493487	19.159286846328854
45	19.16501646847535	31.86165810574001	29.242952732292622	19.730372693492022
46	20.162171761339458	31.52783222594145	29.42395518162232	18.88604083109677
47	19.986965947280773	30.30031931753834	29.981236325134276	19.731478410046606
48	20.699516500306586	28.799560393903146	31.149610151148106	19.351312954642168
49	19.60298074377867	29.095356325536358	31.687591514798175	19.6140714158868
50	19.140891743647995	31.258305439120253	30.55352840853881	19.047274408692942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2399.0
1	2955.5
2	3512.0
3	22318.0
4	41124.0
5	30532.5
6	19941.0
7	20166.5
8	20392.0
9	20939.0
10	21486.0
11	21709.0
12	21932.0
13	21933.0
14	21934.0
15	21074.5
16	20215.0
17	19614.5
18	19014.0
19	20233.0
20	21452.0
21	23492.5
22	25533.0
23	30929.0
24	36325.0
25	43077.5
26	49830.0
27	58454.5
28	67079.0
29	78467.5
30	89856.0
31	102173.5
32	114491.0
33	127979.5
34	141468.0
35	151713.5
36	161959.0
37	168313.5
38	174668.0
39	185562.0
40	196456.0
41	210376.0
42	224296.0
43	232095.5
44	239895.0
45	236048.5
46	232202.0
47	219190.0
48	206178.0
49	193562.5
50	180947.0
51	163829.0
52	146711.0
53	128820.0
54	110929.0
55	99845.5
56	88762.0
57	82938.5
58	77115.0
59	72794.0
60	68473.0
61	60884.0
62	53295.0
63	44290.5
64	35286.0
65	27690.5
66	20095.0
67	15971.5
68	11848.0
69	9548.5
70	7249.0
71	5562.5
72	3876.0
73	3276.5
74	2677.0
75	2221.5
76	1766.0
77	1349.5
78	933.0
79	711.0
80	489.0
81	352.0
82	215.0
83	169.5
84	124.0
85	79.5
86	35.0
87	25.0
88	15.0
89	11.5
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2984490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.500146412908784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44867156120485	16.28709399315802
2	5.729705937875665	2.34919621258728
3	2.0842255249197437	1.2818078525512926
4	1.2073452073263105	0.9900301408445231
5	0.8468036467773813	0.8679799370960705
6	0.6437976745209364	0.7918767952781635
7	0.5240176504574864	0.751970699012885
8	0.4182527552437279	0.685939417607914
9	0.3845310110387175	0.7094647823937799
>10	6.174276876806668	31.78335435466807
>50	2.021871252598399	28.97694021246696
>100	0.5150053691129548	13.514007570046111
>500	9.970214114209607E-4	0.1253256556455477
>1k	3.3234047047365356E-4	0.10836121516947193
>5k	0.0	0.0
>10k+	1.6617023523682678E-4	0.7766511614739989
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22799	0.7639161129707253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01822756986955895	0.0
2	0.0	0.0	0.0	0.062355712366266934	0.0
3	0.0	0.0	0.0	0.10336774457277458	0.0
4	0.0	0.0	0.0	0.1468592623865384	0.0
5	0.0	0.0	0.0	0.24670881792199	0.0
6	0.0	0.0	0.0	0.42060787605252486	0.0
7	0.0	0.0	0.0	0.5217976940783853	0.0
8	0.0	0.0	0.0	0.706251319320889	0.0
9	0.0	0.0	0.0	0.7995335886533378	0.0
10	0.0	0.0	0.0	0.9161364253188987	0.0
11	0.0	0.0	0.0	1.0329067947957606	0.0
12	0.0	0.0	0.0	1.1479683295973517	0.0
13	0.0	0.0	0.0	1.2093523516580722	0.0
14	0.0	0.0	0.0	1.2509004888607433	0.0
15	0.0	0.0	0.0	1.2865179645433558	0.0
16	0.0	0.0	0.0	1.3455230206836009	0.0
17	0.0	0.0	0.0	1.421080318580394	0.0
18	0.0	0.0	0.0	1.5153677847806493	0.0
19	0.0	0.0	0.0	1.573836735924731	0.0
20	0.0	0.0	0.0	1.6335119233101802	0.0
21	0.0	0.0	0.0	1.7083655833995088	0.0
22	0.0	0.0	0.0	1.7969904405777872	0.0
23	3.3506562260218663E-5	0.0	0.0	1.8906412820950984	0.0
24	3.3506562260218663E-5	0.0	0.0	1.9674718293577798	0.0
25	3.3506562260218663E-5	0.0	0.0	2.0329771585765073	0.0
26	3.3506562260218663E-5	0.0	0.0	2.1009284668402306	0.0
27	3.3506562260218663E-5	0.0	0.0	2.1734366675713437	0.0
28	3.3506562260218663E-5	0.0	0.0	2.2491950048416984	0.0
29	3.3506562260218663E-5	0.0	0.0	2.334804271416557	0.0
30	3.3506562260218663E-5	0.0	0.0	2.4395457850420006	0.0
31	3.3506562260218663E-5	0.0	0.0	2.532023896880204	0.0
32	3.3506562260218663E-5	0.0	0.0	2.629595006181961	0.0
33	3.3506562260218663E-5	0.0	0.0	2.7173486927414734	0.0
34	3.3506562260218663E-5	0.0	0.0	2.820113319193564	0.0
35	3.3506562260218663E-5	0.0	0.0	2.9312545862107093	0.0
36	3.3506562260218663E-5	0.0	0.0	3.03559402108903	0.0
37	3.3506562260218663E-5	0.0	0.0	3.1436191778159754	0.0
38	3.3506562260218663E-5	0.0	0.0	3.2570388910668155	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	20	7.8585045E-4	44.0	41
CGTTTTT	17235	0.0	42.212936	1
ACGGGTC	110	0.0	40.0	5
GCGTAAG	150	0.0	39.6	1
CGTAAGG	515	0.0	39.300972	2
GGACGTA	125	0.0	38.72	8
TACGGGA	1780	0.0	38.314606	4
TAGGGCG	770	0.0	38.0	5
TAGGGAC	4055	0.0	37.977806	5
GACGTAC	70	0.0	37.714287	9
CCGCTCG	35	7.2941148E-6	37.714287	19
GGCGATA	1040	0.0	37.653843	8
ACGGGAT	2475	0.0	37.511112	5
TAGGGAT	8860	0.0	37.395035	5
AGGGATT	11325	0.0	37.356293	6
CGTTAGG	385	0.0	37.142857	2
CGAGGGA	2530	0.0	37.04348	4
ACGCCGG	245	0.0	36.816326	27
GTAGGGA	5805	0.0	36.79931	4
AGGGCGA	2175	0.0	36.71724	6
>>END_MODULE