FastQCFastQC Report
Thu 26 May 2016
SRR1547144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547144_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1776145
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58260.3280137601378266No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20520.1155311081020975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG302.5285008E-644.01
TCGTTAG254.4438246E-544.01
CGACTCG207.8576436E-444.017
CGTTTTT50300.040.0636181
TACGCGG501.3496901E-939.62
ACGGGAT8250.039.4666675
TACGGGA6650.039.368424
TTACGAG452.3521352E-839.111111
ATTACGC404.126232E-738.527
ATAGGGC7300.038.273974
TACGGGT1150.038.260874
AGGGCGA15750.038.133336
CGTTAGG1850.038.0540542
CGTAAGG2200.038.02
CGGGATC3600.037.888896
AATACGG1800.037.888892
GGCGATA4650.037.8494648
CGGTAGT3450.037.62318812
GTTAGCG1000.037.3999981
GCGATAA1000.037.3999989
Produced by FastQC (version 0.11.5)