##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547144_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1776145
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.103938023078072	31.0	31.0	33.0	30.0	34.0
2	31.477017360632157	31.0	31.0	34.0	30.0	34.0
3	31.636671555531784	31.0	31.0	34.0	30.0	34.0
4	35.402077533084295	37.0	35.0	37.0	33.0	37.0
5	35.20059848717306	37.0	35.0	37.0	32.0	37.0
6	35.2744826576659	37.0	35.0	37.0	32.0	37.0
7	35.717392442621524	37.0	35.0	37.0	35.0	37.0
8	35.75860923516942	37.0	35.0	37.0	35.0	37.0
9	37.55508362211418	39.0	37.0	39.0	35.0	39.0
10	36.87660748418626	39.0	37.0	39.0	32.0	39.0
11	36.5092596606696	38.0	35.0	39.0	32.0	39.0
12	35.84493157934741	37.0	35.0	39.0	31.0	39.0
13	35.5850547111863	37.0	35.0	39.0	30.0	39.0
14	36.521330184191044	38.0	35.0	40.0	31.0	41.0
15	36.77366206024846	38.0	35.0	40.0	31.0	41.0
16	36.87207407052915	38.0	35.0	40.0	32.0	41.0
17	36.78034507317815	38.0	35.0	40.0	31.0	41.0
18	36.71829214394095	38.0	35.0	40.0	31.0	41.0
19	36.69055848480839	38.0	35.0	40.0	31.0	41.0
20	36.54120806578292	38.0	35.0	40.0	31.0	41.0
21	36.41182110694791	38.0	35.0	40.0	31.0	41.0
22	36.339270723955536	38.0	35.0	40.0	30.0	41.0
23	36.20230724405947	38.0	34.0	40.0	30.0	41.0
24	36.15662516292307	38.0	34.0	40.0	30.0	41.0
25	36.00730120570111	38.0	34.0	40.0	30.0	41.0
26	35.90197253039589	38.0	34.0	40.0	30.0	41.0
27	35.71615887216415	37.0	34.0	40.0	29.0	41.0
28	35.670851760413704	37.0	34.0	40.0	29.0	41.0
29	35.593128376343145	37.0	34.0	40.0	29.0	41.0
30	35.597900509248966	37.0	34.0	40.0	29.0	41.0
31	35.51719144551824	37.0	34.0	40.0	29.0	41.0
32	35.363273831809906	37.0	34.0	40.0	28.0	41.0
33	35.16941634832742	37.0	34.0	40.0	27.0	41.0
34	35.076657592707804	37.0	34.0	40.0	27.0	41.0
35	34.86986760652987	37.0	34.0	40.0	26.0	41.0
36	34.6794270738031	37.0	33.0	40.0	25.0	41.0
37	34.78262698146829	37.0	33.0	40.0	25.0	41.0
38	34.76983129192718	37.0	33.0	40.0	25.0	41.0
39	34.71013627828809	37.0	33.0	40.0	25.0	41.0
40	34.65069687441059	37.0	33.0	40.0	24.0	41.0
41	34.687401084933946	37.0	34.0	40.0	24.0	41.0
42	34.476305707022796	37.0	33.0	40.0	24.0	41.0
43	34.52067877341096	37.0	33.0	40.0	24.0	41.0
44	34.325940168173204	36.0	33.0	40.0	23.0	41.0
45	34.08959966669388	36.0	33.0	40.0	23.0	41.0
46	34.195429427214556	36.0	33.0	40.0	23.0	41.0
47	34.0757539502687	36.0	33.0	40.0	23.0	41.0
48	34.14694633602549	36.0	33.0	40.0	23.0	41.0
49	34.08782334775596	36.0	33.0	40.0	24.0	41.0
50	33.60483744288895	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	3.0
12	6.0
13	19.0
14	12.0
15	59.0
16	140.0
17	373.0
18	845.0
19	1572.0
20	3009.0
21	4860.0
22	7382.0
23	10912.0
24	15179.0
25	21324.0
26	27873.0
27	33962.0
28	39051.0
29	44843.0
30	53658.0
31	65900.0
32	81638.0
33	103863.0
34	159910.0
35	208105.0
36	142765.0
37	174923.0
38	242007.0
39	331949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.54450227881169	24.17584149942713	29.24885074135276	13.030805480408414
2	31.014979069839455	25.77272688885198	29.787545498819075	13.424748542489493
3	29.476985268657685	25.667442691897342	31.27227788271791	13.583294156727069
4	26.423574651844305	28.45218155049278	30.764999479209187	14.35924431845373
5	23.43451688910534	31.89429917039431	29.927680454016986	14.743503486483366
6	21.81651835857996	40.58170926360179	27.166081598067727	10.435690779750527
7	87.61368018939895	4.203654543970227	6.505718846152764	1.676946420478058
8	88.19127942819985	3.4807405926880968	6.458763220345186	1.8692167587668798
9	83.04023601676664	6.177367275757328	7.987298334313922	2.795098373162101
10	44.371095828324826	30.302537236543188	14.582536898732931	10.743830036399055
11	37.13187830948487	23.876260102638017	24.631772743779365	14.360088844097751
12	34.38964724163849	23.684946893412416	26.592310875519736	15.333094989429355
13	22.412978670097317	33.34018337466817	26.985015299989584	17.261822655244927
14	17.615904106928205	35.712343305304465	29.551641335589157	17.120111252178173
15	17.0494526066284	26.303595708683698	40.52473193348516	16.122219751202746
16	19.918869236464364	24.038972043386096	38.75246671865191	17.289692001497624
17	20.08287611653328	23.560745322031703	29.55068420652593	26.805694354909086
18	22.222678891644545	25.6819685329745	33.44681881265325	18.648533762727705
19	27.959992005157236	26.16385486545299	27.778193784854277	18.097959344535496
20	29.435997624067856	25.633380157588487	27.259317229167664	17.671304989175994
21	24.624228314692775	27.645997370710162	29.959547221651384	17.770227092945678
22	23.74744179106999	25.24906468784925	28.67547413077198	22.328019390308786
23	20.71536952219554	29.477886096011307	28.23722162323459	21.569522758558563
24	20.68620523662201	26.04545237016122	35.54388859017704	17.72445380303973
25	19.370265378108208	27.749874024924765	33.38832133637738	19.491539260589647
26	19.267739964924036	33.06194032581799	27.986679015508305	19.683640693749666
27	18.802913050454777	33.341365710569804	29.936407219005208	17.919314019970216
28	17.41389357287834	29.883596215399084	35.30021479102213	17.40229542070045
29	18.20932412612709	27.418031748534045	34.51959158739855	19.853052537940314
30	19.760942941032404	30.877715501831215	31.243282502273185	18.1180590548632
31	26.100853252409014	28.329500125271306	27.706127596564468	17.863519025755217
32	27.504173364224204	27.2406813632896	28.38715307590315	16.86799219658305
33	26.339516199409395	27.70685951878929	27.076392974672675	18.877231307128643
34	21.297585501183743	28.17664098370347	29.190465868496098	21.335307646616688
35	21.07063330978045	27.35401670471724	31.416297655878324	20.15905232962399
36	26.39086335856588	27.929194970005266	27.256727350526	18.423214320902854
37	20.8652446731545	32.4361468235983	28.46862164969639	18.22998685355081
38	21.052841969546403	32.971238271650115	25.887863884986867	20.088055873816607
39	21.533714871252066	31.71295136376816	27.463242021343977	19.290091743635795
40	23.88318521291899	27.24006204448398	27.80251612340209	21.07423661919494
41	18.780899082000623	26.584372334465932	29.07656750997244	25.558161073561
42	21.464688975280737	27.25712146249321	26.98405817092636	24.294131391299697
43	21.571493318394612	27.47697963848672	27.218667394835443	23.73285964828322
44	19.857725579837233	29.724937997742302	29.413308035098485	21.00402838732198
45	19.245050375954666	33.71121164094148	26.77805021549479	20.265687767609066
46	21.75757046862728	31.71238834667215	27.042161535235014	19.487879649465555
47	20.831069535426444	28.647435879390475	28.784417938850716	21.737076646332365
48	21.711403066754123	27.101165726897296	31.31698144014143	19.87044976620715
49	21.159984122917894	26.971559191394846	31.760301101542947	20.108155584144313
50	19.8962922509142	32.6514445611141	28.21053461288352	19.24172857508818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1885.0
1	2725.5
2	3566.0
3	8003.5
4	12441.0
5	9739.0
6	7037.0
7	7380.0
8	7723.0
9	8161.5
10	8600.0
11	8763.5
12	8927.0
13	8896.0
14	8865.0
15	8572.5
16	8280.0
17	8100.0
18	7920.0
19	8815.5
20	9711.0
21	9419.0
22	9127.0
23	11707.5
24	14288.0
25	16870.5
26	19453.0
27	22918.5
28	26384.0
29	31125.5
30	35867.0
31	40433.5
32	45000.0
33	53055.0
34	61110.0
35	68621.5
36	76133.0
37	77677.0
38	79221.0
39	91319.5
40	103418.0
41	121209.0
42	139000.0
43	152945.0
44	166890.0
45	164818.0
46	162746.0
47	146208.5
48	129671.0
49	125795.0
50	121919.0
51	110103.0
52	98287.0
53	88234.0
54	78181.0
55	71783.0
56	65385.0
57	61867.0
58	58349.0
59	55946.5
60	53544.0
61	50080.0
62	46616.0
63	40748.0
64	34880.0
65	29410.5
66	23941.0
67	19765.0
68	15589.0
69	13301.0
70	11013.0
71	8748.5
72	6484.0
73	5173.5
74	3863.0
75	3209.0
76	2555.0
77	1904.0
78	1253.0
79	917.0
80	581.0
81	421.5
82	262.0
83	179.0
84	96.0
85	70.0
86	44.0
87	28.0
88	12.0
89	10.5
90	9.0
91	10.5
92	12.0
93	8.0
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1776145.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.450057136722965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.59920183310788	15.871091379649377
2	5.548023089636406	2.1581873217857277
3	1.7755318680166026	1.0360258884298614
4	0.9618729125628526	0.7483393243045446
5	0.6289442704479971	0.6116500998014042
6	0.5286456935649048	0.6169313366951963
7	0.398133846880263	0.5420608248919099
8	0.33625478611498855	0.523213984194647
9	0.2985614575079337	0.5226333666617337
>10	5.061487734760958	25.135516686829444
>50	1.9334141314559339	26.24492882004727
>100	0.9281770983992488	25.387310422341216
>500	8.756387720592572E-4	0.08975455125828673
>1k	5.837591813728381E-4	0.1816096201614543
>5k	2.9187959068641904E-4	0.33074637294799397
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5826	0.3280137601378266	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2052	0.1155311081020975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012780488079520534	0.0
2	0.0	0.0	0.0	0.056470614730216286	0.0
3	0.0	0.0	0.0	0.08439626269251665	0.0
4	0.0	0.0	0.0	0.1414861962283485	0.0
5	0.0	0.0	0.0	0.22261695976398324	0.0
6	0.0	0.0	0.0	0.3592612089666103	0.0
7	0.0	0.0	0.0	0.4235577613314228	0.0
8	0.0	0.0	0.0	0.5808084362481667	0.0
9	0.0	0.0	0.0	0.6488772031562738	0.0
10	0.0	0.0	0.0	0.767223396738442	0.0
11	0.0	0.0	0.0	0.9031357237162506	0.0
12	0.0	0.0	0.0	1.0117980232469759	0.0
13	0.0	0.0	0.0	1.063877104628282	0.0
14	0.0	0.0	0.0	1.086397788468847	0.0
15	0.0	0.0	0.0	1.1144923415599515	0.0
16	0.0	0.0	0.0	1.1790141007631696	0.0
17	0.0	0.0	0.0	1.2510239873433757	0.0
18	0.0	0.0	0.0	1.3483133415346158	0.0
19	0.0	0.0	0.0	1.4035453186536009	0.0
20	0.0	0.0	0.0	1.4578201667093622	5.630170960141205E-5
21	0.0	0.0	0.0	1.531744311416016	5.630170960141205E-5
22	0.0	0.0	0.0	1.6101162911811817	5.630170960141205E-5
23	0.0	0.0	0.0	1.7023384915082946	5.630170960141205E-5
24	0.0	0.0	0.0	1.7692812242243736	5.630170960141205E-5
25	0.0	0.0	0.0	1.827553493661835	5.630170960141205E-5
26	0.0	0.0	0.0	1.8844182203592612	5.630170960141205E-5
27	0.0	0.0	0.0	1.9463501009208144	5.630170960141205E-5
28	0.0	0.0	0.0	2.0082819814823676	5.630170960141205E-5
29	0.0	0.0	0.0	2.0801229629337694	5.630170960141205E-5
30	0.0	0.0	0.0	2.162999079467048	5.630170960141205E-5
31	0.0	0.0	0.0	2.250323031058838	5.630170960141205E-5
32	0.0	0.0	0.0	2.3249790979903104	5.630170960141205E-5
33	0.0	0.0	0.0	2.4089249470060157	5.630170960141205E-5
34	0.0	0.0	0.0	2.4944472438905607	5.630170960141205E-5
35	0.0	0.0	0.0	2.5962407348499137	5.630170960141205E-5
36	0.0	0.0	0.0	2.6875058061138026	5.630170960141205E-5
37	0.0	0.0	0.0	2.7794464978929083	5.630170960141205E-5
38	0.0	0.0	0.0	2.882140816205884	5.630170960141205E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	2.5285008E-6	44.0	1
TCGTTAG	25	4.4438246E-5	44.0	1
CGACTCG	20	7.8576436E-4	44.0	17
CGTTTTT	5030	0.0	40.063618	1
TACGCGG	50	1.3496901E-9	39.6	2
ACGGGAT	825	0.0	39.466667	5
TACGGGA	665	0.0	39.36842	4
TTACGAG	45	2.3521352E-8	39.11111	1
ATTACGC	40	4.126232E-7	38.5	27
ATAGGGC	730	0.0	38.27397	4
TACGGGT	115	0.0	38.26087	4
AGGGCGA	1575	0.0	38.13333	6
CGTTAGG	185	0.0	38.054054	2
CGTAAGG	220	0.0	38.0	2
CGGGATC	360	0.0	37.88889	6
AATACGG	180	0.0	37.88889	2
GGCGATA	465	0.0	37.849464	8
CGGTAGT	345	0.0	37.623188	12
GTTAGCG	100	0.0	37.399998	1
GCGATAA	100	0.0	37.399998	9
>>END_MODULE