FastQCFastQC Report
Thu 26 May 2016
SRR1547143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547143_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1674029
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64640.38613429038565045No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39260.2345240136222252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG1000.044.01
GATCGGA207.857513E-444.09
GCGCGTA254.4437147E-544.042
TAGCGCG254.4437147E-544.01
TATATCG207.857513E-444.028
TTGTACG600.044.01
TCGATCT207.857513E-444.040
CCGTCGA254.4437147E-544.036
CGTTATC207.857513E-444.044
CCCGTTA254.4437147E-544.032
CCCCGAT302.5284116E-644.040
ACGTGCG351.4466059E-744.01
TCGTATA207.857513E-444.013
GCGTGTC254.4437147E-544.036
CGGTATA351.4466059E-744.02
TGTCGGT207.857513E-444.040
TACGTCC207.857513E-444.021
AATCGCG551.8189894E-1244.01
TACCGAT254.4437147E-544.038
TATAGCG1350.042.370371
Produced by FastQC (version 0.11.5)