##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547143_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1674029
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.032412819610652	31.0	31.0	33.0	30.0	34.0
2	31.400429741659195	31.0	31.0	34.0	30.0	34.0
3	31.550126073084755	31.0	31.0	34.0	30.0	34.0
4	35.312637355744734	37.0	35.0	37.0	33.0	37.0
5	35.09930353655761	37.0	35.0	37.0	32.0	37.0
6	35.16324448381719	37.0	35.0	37.0	32.0	37.0
7	35.68589671982982	37.0	35.0	37.0	35.0	37.0
8	35.74705754798752	37.0	35.0	37.0	35.0	37.0
9	37.570977563710066	39.0	37.0	39.0	35.0	39.0
10	36.83765155800766	39.0	37.0	39.0	32.0	39.0
11	36.33055819224159	38.0	35.0	39.0	32.0	39.0
12	35.09185085801978	35.0	34.0	39.0	30.0	39.0
13	34.49349264558738	35.0	33.0	39.0	26.0	39.0
14	35.301895606348516	36.0	33.0	40.0	27.0	41.0
15	35.78928740183115	37.0	34.0	40.0	30.0	41.0
16	36.04999674438137	36.0	34.0	40.0	31.0	41.0
17	36.02566801411445	36.0	34.0	40.0	31.0	41.0
18	36.01546568189679	36.0	35.0	40.0	31.0	41.0
19	35.96012673615571	36.0	34.0	40.0	31.0	41.0
20	35.74046506960154	36.0	34.0	40.0	30.0	41.0
21	35.557306952269045	36.0	34.0	40.0	30.0	41.0
22	35.46885687165515	35.0	34.0	40.0	30.0	41.0
23	35.38393839055357	35.0	34.0	40.0	29.0	41.0
24	35.33524269890187	35.0	34.0	40.0	29.0	41.0
25	35.20603824664925	35.0	34.0	40.0	29.0	41.0
26	35.05995415850024	35.0	34.0	40.0	29.0	41.0
27	34.93422276436071	35.0	34.0	40.0	29.0	41.0
28	34.9944708245795	36.0	34.0	40.0	29.0	41.0
29	34.98041192834771	36.0	34.0	40.0	29.0	41.0
30	34.949967413945636	36.0	34.0	40.0	29.0	41.0
31	34.77909462739296	35.0	34.0	40.0	28.0	41.0
32	34.5511159006206	35.0	33.0	40.0	27.0	41.0
33	34.354486093132195	35.0	33.0	40.0	26.0	41.0
34	34.32417956917114	35.0	33.0	40.0	26.0	41.0
35	34.07697118747644	35.0	33.0	39.0	25.0	41.0
36	33.75386149224416	35.0	33.0	39.0	23.0	41.0
37	33.79631953807252	35.0	33.0	39.0	23.0	41.0
38	33.85272118941786	35.0	33.0	39.0	23.0	41.0
39	33.85362917846704	35.0	33.0	39.0	23.0	41.0
40	33.780746928517964	35.0	33.0	39.0	23.0	41.0
41	33.87671599476472	35.0	33.0	39.0	23.0	41.0
42	33.63705885620858	35.0	33.0	39.0	23.0	41.0
43	33.68945161642959	35.0	33.0	39.0	23.0	41.0
44	33.524234048514096	35.0	33.0	39.0	23.0	41.0
45	33.338909899410346	35.0	32.0	39.0	23.0	41.0
46	33.42895194766638	35.0	32.0	39.0	23.0	41.0
47	33.30226059405184	35.0	32.0	39.0	23.0	41.0
48	33.4103811821659	35.0	32.0	39.0	23.0	41.0
49	33.39238448079454	35.0	32.0	39.0	23.0	41.0
50	32.83768859440308	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	2.0
12	4.0
13	8.0
14	10.0
15	34.0
16	131.0
17	407.0
18	905.0
19	1857.0
20	3404.0
21	5557.0
22	8488.0
23	12093.0
24	17217.0
25	23772.0
26	31347.0
27	37339.0
28	42623.0
29	49377.0
30	58447.0
31	71422.0
32	88475.0
33	113711.0
34	190432.0
35	272140.0
36	112999.0
37	126604.0
38	171217.0
39	234003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.754766494487253	23.869060810774485	31.143307553214434	13.232865141523833
2	29.234141105082408	25.9815690170242	31.66438574242143	13.119904135471966
3	27.729627145049456	25.44191289398212	32.71299362197429	14.115466338994128
4	24.176164212208988	28.357334311412764	32.45427647908131	15.012224997296942
5	22.677623864341655	32.111689821383024	31.236316694633125	13.974369619642193
6	20.844800179686253	40.50712383118811	27.989120857523975	10.658955131601664
7	87.7755403281544	4.422742975181434	6.173608700924536	1.6281079957396198
8	89.51917798317712	3.2587249085887997	5.51400244559682	1.7080946626372662
9	85.77013898803426	5.0081569674121535	7.040081145547658	2.1816228990059314
10	55.79102870977742	23.62402323974077	11.417723348878663	9.16722470160314
11	50.452590725728165	19.296320434114346	18.945251247140877	11.305837593016609
12	45.196767797929425	21.832656423514766	21.420118767357078	11.55045701119873
13	20.841335484630193	45.52352438338882	21.25279789059807	12.382342241382915
14	13.601377276020903	46.92111068565718	25.821356738742278	13.656155299579636
15	12.074880423218474	24.17538764262746	51.151742293592285	12.597989640561783
16	13.654900841024856	18.5303838822386	49.32142752604644	18.4932877506901
17	14.039840408977383	19.057196739124592	29.059293476994725	37.84366937490331
18	19.862977284145018	23.438184165268343	37.47324568451323	19.225592866073406
19	30.546722906234002	24.522155828841676	25.06868160587421	19.862439659050114
20	33.51154609627432	22.989924308360248	25.302608258279875	18.195921337085558
21	21.521729910294265	30.405805395247036	27.711108947336037	20.361355747122662
22	22.246388802105578	24.863368555741864	24.180166532359955	28.710076109792602
23	17.186440617217503	33.19470570700985	25.0333775579754	24.585476117797242
24	18.14156146637842	23.722109951500244	42.299088008630676	15.837240573490662
25	15.64052952487681	25.74656711442872	39.04090072513678	19.572002635557688
26	13.98273267667406	39.102548402685976	27.76074966443234	19.153969256207628
27	15.430318112768656	39.78162863367361	27.884881325233913	16.90317192832382
28	12.836038085361723	31.27287520108672	39.78324150895833	16.10784520459323
29	13.493135423579877	26.409040703595938	38.48983500285838	21.607988869965812
30	16.54403836492677	36.13067635029023	29.98382943186767	17.34145585291533
31	29.951332981686697	29.709282216735794	24.25573272625504	16.083652075322473
32	31.583025144725692	27.13823954065312	25.42799437763623	15.850740936984964
33	29.716689495821157	29.319145606199175	22.759522087132304	18.204642810847364
34	19.553066284992674	28.657508322735147	26.131566418502906	25.657858973769272
35	19.454561420381605	26.74003855369292	32.89996768275819	20.905432343167295
36	32.21264386698199	25.793041817077246	25.267244474259403	16.72706984168136
37	19.705214186851006	36.288797864314176	28.20255802020156	15.803429928633255
38	18.515509587946205	36.87576499570796	24.29294833004685	20.315777086298983
39	20.03071631375562	34.874127031252144	26.27720308310071	18.817953571891525
40	25.652900875671808	26.36895776596463	25.104105126016336	22.87403623234723
41	16.3008526136644	24.971789616547863	27.605794164856164	31.121563604931573
42	20.91504985875394	24.691388261493678	24.758770606721868	29.634791273030515
43	20.535426805628816	25.484743693209616	26.58538173472503	27.394447766436542
44	17.566242878707598	30.82575033049009	29.951034301078415	21.656972489723895
45	16.16716317339783	41.74993384224527	21.643770806837875	20.439132177519028
46	21.39574642972135	36.523321877936404	24.378609928501835	17.702321763840413
47	20.184596563141977	29.17542049749437	25.629723260469206	25.010259678894453
48	21.578001336894403	25.43139933657063	33.138016127558124	19.852583198976838
49	21.046230381910945	24.978002173200107	33.72665587035828	20.24911157453067
50	19.425230984648415	36.56483848248746	25.582830404969087	18.427100127895034
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2167.0
1	2612.0
2	3057.0
3	6752.5
4	10448.0
5	7822.5
6	5197.0
7	5931.0
8	6665.0
9	7809.0
10	8953.0
11	9477.5
12	10002.0
13	9853.0
14	9704.0
15	9158.0
16	8612.0
17	8322.0
18	8032.0
19	7639.5
20	7247.0
21	7950.0
22	8653.0
23	8239.5
24	7826.0
25	9029.5
26	10233.0
27	12088.0
28	13943.0
29	17056.0
30	20169.0
31	23991.5
32	27814.0
33	35759.5
34	43705.0
35	49404.5
36	55104.0
37	56232.0
38	57360.0
39	72900.0
40	88440.0
41	127679.0
42	166918.0
43	196267.0
44	225616.0
45	216152.0
46	206688.0
47	182525.5
48	158363.0
49	139130.5
50	119898.0
51	106342.5
52	92787.0
53	81088.5
54	69390.0
55	61239.5
56	53089.0
57	47432.0
58	41775.0
59	40020.5
60	38266.0
61	34769.0
62	31272.0
63	26428.0
64	21584.0
65	17014.0
66	12444.0
67	10333.5
68	8223.0
69	6848.0
70	5473.0
71	4630.5
72	3788.0
73	2987.5
74	2187.0
75	1792.0
76	1397.0
77	1105.0
78	813.0
79	587.5
80	362.0
81	271.5
82	181.0
83	164.0
84	147.0
85	83.0
86	19.0
87	13.0
88	7.0
89	6.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1674029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.949100700194172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.78551266783116	15.497619153597384
2	7.6729275774676085	2.9079015466146125
3	2.435217492460873	1.384355444745463
4	1.2062284694291256	0.9142777893861436
5	0.6929187767979516	0.6565093839299877
6	0.4742625606718066	0.5392109412301207
7	0.3650523171167687	0.4842189182519407
8	0.2648657797429785	0.40151746659081183
9	0.22575448638447165	0.385006004641797
>10	2.4035279780501435	10.696956679918522
>50	1.1370185630023903	15.997306821244539
>100	1.318240761780845	47.177897404421806
>500	0.01624312124929235	1.930342039121994
>1k	0.0019109554410932175	0.6367684494224216
>5k	3.1849257351553625E-4	0.3901119568824314
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6464	0.38613429038565045	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3926	0.2345240136222252	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04611628591858325	0.0
2	0.0	0.0	0.0	0.18667538017561225	0.0
3	0.0	0.0	0.0	0.2636155048687926	0.0
4	0.0	0.0	0.0	0.42836772839658094	0.0
5	0.0	0.0	0.0	0.698852887255836	0.0
6	0.0	0.0	0.0	1.0405435031292767	0.0
7	0.0	0.0	0.0	1.2199908125844894	0.0
8	0.0	0.0	0.0	1.6554074033365014	0.0
9	0.0	0.0	0.0	1.804747707476991	0.0
10	0.0	0.0	0.0	2.057132821474419	0.0
11	0.0	0.0	0.0	2.348047733940093	0.0
12	0.0	0.0	0.0	2.570564787109423	0.0
13	0.0	0.0	0.0	2.6607065946886226	0.0
14	0.0	0.0	0.0	2.693143308747937	0.0
15	0.0	0.0	0.0	2.745651359683733	0.0
16	0.0	0.0	0.0	2.8699024927286207	0.0
17	0.0	0.0	0.0	3.0225880196818573	0.0
18	0.0	0.0	0.0	3.2329786401549794	0.0
19	0.0	0.0	0.0	3.329571948873048	0.0
20	0.0	0.0	0.0	3.442712163289883	0.0
21	0.0	0.0	0.0	3.596891093284525	0.0
22	0.0	0.0	0.0	3.7492779396294806	0.0
23	0.0	0.0	0.0	3.930517332734379	0.0
24	0.0	0.0	0.0	4.046943033842305	0.0
25	0.0	0.0	0.0	4.151122830010711	0.0
26	0.0	0.0	0.0	4.255541570665741	0.0
27	0.0	0.0	0.0	4.353926963033496	0.0
28	0.0	0.0	0.0	4.473339470224231	0.0
29	0.0	0.0	0.0	4.5843889203831	0.0
30	0.0	0.0	0.0	4.736835502849711	0.0
31	0.0	0.0	0.0	4.8844434594621715	0.0
32	0.0	0.0	0.0	5.004990952964375	0.0
33	0.0	0.0	0.0	5.1392777544475035	0.0
34	0.0	0.0	0.0	5.272907458592414	0.0
35	0.0	0.0	0.0	5.454146851697312	0.0
36	0.0	0.0	0.0	5.6096997124900465	0.0
37	0.0	0.0	0.0	5.747451209029235	0.0
38	0.0	0.0	0.0	5.892968401383728	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	100	0.0	44.0	1
GATCGGA	20	7.857513E-4	44.0	9
GCGCGTA	25	4.4437147E-5	44.0	42
TAGCGCG	25	4.4437147E-5	44.0	1
TATATCG	20	7.857513E-4	44.0	28
TTGTACG	60	0.0	44.0	1
TCGATCT	20	7.857513E-4	44.0	40
CCGTCGA	25	4.4437147E-5	44.0	36
CGTTATC	20	7.857513E-4	44.0	44
CCCGTTA	25	4.4437147E-5	44.0	32
CCCCGAT	30	2.5284116E-6	44.0	40
ACGTGCG	35	1.4466059E-7	44.0	1
TCGTATA	20	7.857513E-4	44.0	13
GCGTGTC	25	4.4437147E-5	44.0	36
CGGTATA	35	1.4466059E-7	44.0	2
TGTCGGT	20	7.857513E-4	44.0	40
TACGTCC	20	7.857513E-4	44.0	21
AATCGCG	55	1.8189894E-12	44.0	1
TACCGAT	25	4.4437147E-5	44.0	38
TATAGCG	135	0.0	42.37037	1
>>END_MODULE