FastQCFastQC Report
Thu 26 May 2016
SRR1547142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547142_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4765624
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141000.29586891454298536No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA68270.1432551120273022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG24000.038.8666655
AGGGCGA57400.038.864116
CGTAAGG8000.038.7752
CGTTTTT122450.038.4303781
GGGCGAT100950.037.9415557
TATACGG3950.037.3164562
GGCGATA24300.036.3950658
GCGATGT11250.036.3733339
AAGGGCG21650.035.9722865
CGGGATT18200.035.90116
TACGGGT4200.035.619054
GCGATAT6350.035.3385859
TTACGGG15050.035.2292333
CGACGGT2500.035.228
TATAGCG2250.035.21
CGCGTAG250.002353485235.21
TACGGGA15950.035.1724134
GGCGATT32050.035.0764478
TAGGGAC36500.034.95895
TAGTAGG26450.034.7674872
Produced by FastQC (version 0.11.5)