##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547142_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4765624
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02417815589312	31.0	31.0	33.0	30.0	34.0
2	31.39955040515156	31.0	31.0	34.0	30.0	34.0
3	31.5660184269678	31.0	31.0	34.0	30.0	34.0
4	35.333954797944614	37.0	35.0	37.0	33.0	37.0
5	35.130684670045305	37.0	35.0	37.0	32.0	37.0
6	35.1998947042402	37.0	35.0	37.0	32.0	37.0
7	35.67504528263245	37.0	35.0	37.0	35.0	37.0
8	35.715329409118304	37.0	35.0	37.0	35.0	37.0
9	37.49876532433108	39.0	37.0	39.0	35.0	39.0
10	36.74882407844177	39.0	37.0	39.0	32.0	39.0
11	36.402072215516796	38.0	35.0	39.0	32.0	39.0
12	35.77747342215836	37.0	35.0	39.0	31.0	39.0
13	35.54539594395194	37.0	35.0	39.0	30.0	39.0
14	36.46570984198502	38.0	35.0	40.0	30.0	41.0
15	36.70853596506984	38.0	35.0	40.0	31.0	41.0
16	36.784002472708714	38.0	35.0	40.0	31.0	41.0
17	36.698759071215015	38.0	35.0	40.0	31.0	41.0
18	36.64685862753755	38.0	35.0	40.0	31.0	41.0
19	36.616468063783465	38.0	35.0	40.0	31.0	41.0
20	36.487987722069555	38.0	35.0	40.0	31.0	41.0
21	36.36508881103503	38.0	35.0	40.0	30.0	41.0
22	36.292458867925795	38.0	35.0	40.0	30.0	41.0
23	36.12279441265194	38.0	34.0	40.0	30.0	41.0
24	36.074474192676554	38.0	34.0	40.0	30.0	41.0
25	35.93638839320937	38.0	34.0	40.0	30.0	41.0
26	35.79987132849759	38.0	34.0	40.0	30.0	41.0
27	35.651187756314805	37.0	34.0	40.0	29.0	41.0
28	35.63160270302483	37.0	34.0	40.0	29.0	41.0
29	35.52113972902604	37.0	34.0	40.0	29.0	41.0
30	35.508740303473374	37.0	34.0	40.0	29.0	41.0
31	35.45398608870528	37.0	34.0	40.0	29.0	41.0
32	35.28027599323824	37.0	34.0	40.0	27.0	41.0
33	35.14607069294598	37.0	34.0	40.0	27.0	41.0
34	35.085212765421694	37.0	34.0	40.0	27.0	41.0
35	34.89999148065395	37.0	33.0	40.0	26.0	41.0
36	34.69967206812791	37.0	33.0	40.0	25.0	41.0
37	34.789030985239286	37.0	33.0	40.0	25.0	41.0
38	34.7744005821693	37.0	33.0	40.0	25.0	41.0
39	34.697134100382236	37.0	33.0	40.0	25.0	41.0
40	34.66775095979037	37.0	33.0	40.0	24.0	41.0
41	34.699377458230025	37.0	33.0	40.0	24.0	41.0
42	34.46074532946787	37.0	33.0	40.0	24.0	41.0
43	34.51657117724772	37.0	33.0	40.0	24.0	41.0
44	34.337778011861616	36.0	33.0	40.0	24.0	41.0
45	34.150773959506665	36.0	33.0	40.0	23.0	41.0
46	34.22667566723686	36.0	33.0	40.0	23.0	41.0
47	34.084239335709235	36.0	33.0	40.0	23.0	41.0
48	34.127851043221206	36.0	33.0	40.0	23.0	41.0
49	34.05450660815876	36.0	33.0	40.0	24.0	41.0
50	33.547755131332224	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	6.0
12	8.0
13	19.0
14	47.0
15	128.0
16	370.0
17	952.0
18	2375.0
19	4719.0
20	8327.0
21	13076.0
22	20288.0
23	29437.0
24	41522.0
25	57382.0
26	75706.0
27	92262.0
28	106612.0
29	125434.0
30	150588.0
31	182623.0
32	223489.0
33	282430.0
34	421632.0
35	545130.0
36	387161.0
37	467687.0
38	646260.0
39	879948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.30874949429498	23.893932882661325	28.605886658284412	13.191430964759284
2	31.255718033986735	26.066680879565823	29.139478901398853	13.53812218504859
3	29.405110432547765	26.361059957730614	30.60950675084732	13.624322858874304
4	25.984991682096613	28.714980451668033	30.435867370149218	14.864160496086138
5	23.21729536362919	32.55281994550976	29.66916819287464	14.560716497986412
6	21.14858410986683	41.269495872943395	26.987001072682194	10.594918944507583
7	87.85214695913903	4.298555656090367	6.067935699501262	1.7813616852693372
8	88.8074678153375	3.2765069170375174	5.76929694831149	2.1467283193134836
9	83.74691331082771	5.389871294923813	7.901630510506075	2.961584883742402
10	43.04284181882582	28.686295855485035	15.257603201595426	13.013259124093718
11	36.47442601430579	25.863139853249017	23.647669224428952	14.014764908016241
12	32.720311128196435	24.730780271376844	26.5009786756152	16.047929924811523
13	21.791270146364884	34.21031537527929	27.847496990950187	16.150917487405636
14	18.388567793010946	36.35108434907999	28.921543117963143	16.33880473994591
15	17.607159104453057	26.071318257588093	40.09059464196084	16.230927995998005
16	19.568749024262093	24.35145953604397	38.58732875275095	17.49246268694299
17	20.058485520469095	24.04184635632186	28.97467362091512	26.92499450229393
18	22.55131751896499	25.206080043243023	33.26611583288988	18.976486604902107
19	27.42528575481406	26.059525468228294	27.64080842298931	18.874380353968338
20	29.360121570648463	25.902882812408194	26.651850838421158	18.08514477852218
21	24.15329031413305	28.569647962155635	28.882954257406794	18.39410746630452
22	23.477995746202385	24.954549498659567	27.854295680901387	23.71315907423666
23	20.584166942251425	30.130765666783617	27.95335930824589	21.33170808271907
24	19.928030411127693	26.423905872557295	35.689932734936704	17.958130981378304
25	19.47077654468754	27.703759255870796	33.072395136502585	19.753069062939083
26	19.045082029131965	32.99777741592707	28.371226936913192	19.585913618027774
27	18.655500308039407	33.00894069695805	29.4889609419459	18.84659805305664
28	17.566366964745857	29.852397083781685	34.78709188975043	17.794144061722033
29	18.506579621052772	27.218387350743573	34.38047147655795	19.894561551645705
30	19.62498510163622	30.65189364498752	30.662343483245845	19.060777770130414
31	25.77278442445313	27.910426840220715	27.36690934912196	18.9498793862042
32	26.093791704926783	27.633988749427147	28.307772497368656	17.964447048277414
33	25.49945190808171	27.903271428883187	26.76927512535609	19.82800153767901
34	20.330454102128073	28.037377686531713	29.230925477964693	22.401242733375522
35	20.221842931796548	27.68949040041766	31.606626960079097	20.482039707706694
36	26.32005798191381	27.450298219078974	27.431035264217236	18.798608534789988
37	20.56580628266099	32.33626908039744	28.536829594613423	18.56109504232814
38	20.08043437753377	32.27778775665055	26.956973525397725	20.68480434041796
39	20.458852817595346	31.118170464140686	28.347767259859356	20.075209458404608
40	23.581109210462262	26.92931712615179	28.15843213816281	21.331141525223142
41	18.737609177727826	27.434728379746282	28.675111590843088	25.152550851682804
42	21.013302770004515	27.675683184405653	27.62649760031425	23.684516445275584
43	21.078813603423182	27.637870717454838	28.039266211518154	23.244049467603823
44	19.50642769970942	29.983334816175173	29.689879016892647	20.82035846722276
45	18.78129705574758	34.298593426590095	26.453618665677357	20.466490851984968
46	21.21178674607984	31.72440796840036	27.553096929174437	19.51070835634536
47	20.71330008410231	28.94838115638162	28.132832132791002	22.20548662672506
48	21.650847821817248	27.134641759400235	31.2908445987346	19.92366582004791
49	21.310430701205128	26.835289565437808	31.40675806568038	20.44752166767668
50	19.565475580952253	32.36484875852564	27.84430748208419	20.225368178437915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4059.0
1	6203.0
2	8347.0
3	18680.0
4	29013.0
5	22432.5
6	15852.0
7	16533.0
8	17214.0
9	18393.0
10	19572.0
11	19754.5
12	19937.0
13	20209.0
14	20481.0
15	20729.5
16	20978.0
17	20647.0
18	20316.0
19	21646.0
20	22976.0
21	24490.0
22	26004.0
23	30895.5
24	35787.0
25	39151.5
26	42516.0
27	54344.5
28	66173.0
29	79678.5
30	93184.0
31	110874.0
32	128564.0
33	146056.0
34	163548.0
35	181802.0
36	200056.0
37	212508.5
38	224961.0
39	259942.0
40	294923.0
41	341413.5
42	387904.0
43	416679.5
44	445455.0
45	437940.5
46	430426.0
47	413181.5
48	395937.0
49	366462.5
50	336988.0
51	305643.5
52	274299.0
53	244941.0
54	215583.0
55	195548.5
56	175514.0
57	163150.5
58	150787.0
59	142523.0
60	134259.0
61	119983.5
62	105708.0
63	92716.0
64	79724.0
65	66624.5
66	53525.0
67	46507.0
68	39489.0
69	32104.5
70	24720.0
71	21072.0
72	17424.0
73	13918.5
74	10413.0
75	8437.5
76	6462.0
77	4847.0
78	3232.0
79	2609.0
80	1986.0
81	1379.5
82	773.0
83	561.5
84	350.0
85	217.0
86	84.0
87	93.0
88	102.0
89	55.0
90	8.0
91	7.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4765624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.456002335397336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.77056291205668	13.456165742475592
2	7.622566851225197	2.5087395581096827
3	2.328362167046312	1.149465997756947
4	1.0641373384076096	0.700457861040765
5	0.6305073776307994	0.5187815439388841
6	0.4346335732885986	0.429139865824756
7	0.31924504344242616	0.3677448026316812
8	0.2490403272082748	0.3278566564917989
9	0.2092727704837862	0.3099413879834631
>10	2.4252115368808034	9.553500858281295
>50	1.1732592976992382	14.266022672389447
>100	1.7571164438077669	54.101660059184155
>500	0.014008959426149045	1.4571936848601053
>1k	0.0018159762219082094	0.406631896766886
>5k	1.2971258727915782E-4	0.14572577213792812
>10k+	1.2971258727915782E-4	0.3009716401266715
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14100	0.29586891454298536	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6827	0.1432551120273022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023942300105925268	0.0
2	0.0	0.0	0.0	0.09939936511986677	0.0
3	0.0	0.0	0.0	0.14604593228504809	0.0
4	0.0	0.0	0.0	0.24129054243473677	0.0
5	0.0	0.0	0.0	0.3976813949233091	0.0
6	0.0	0.0	0.0	0.7196119542792297	0.0
7	0.0	0.0	0.0	0.874177232614239	0.0
8	0.0	0.0	0.0	1.2286323889589275	0.0
9	0.0	0.0	0.0	1.3832606181268183	0.0
10	0.0	2.0983610960495414E-5	0.0	1.6042390251517955	0.0
11	0.0	2.0983610960495414E-5	0.0	1.8017787387338993	0.0
12	0.0	2.0983610960495414E-5	0.0	1.9753132013771963	0.0
13	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.062626006583818	0.0
14	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.1001866702031045	0.0
15	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.1428673348967524	0.0
16	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.22663390985105	0.0
17	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.319948027792373	0.0
18	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.454557892103951	0.0
19	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.5211598732925635	0.0
20	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.590804478070448	0.0
21	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.686909416269517	0.0
22	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.7893933722005766	0.0
23	2.0983610960495414E-5	2.0983610960495414E-5	0.0	2.9102379877220694	0.0
24	2.0983610960495414E-5	2.0983610960495414E-5	0.0	3.003782925383958	0.0
25	2.0983610960495414E-5	2.0983610960495414E-5	0.0	3.0874235986724927	0.0
26	2.0983610960495414E-5	2.0983610960495414E-5	0.0	3.1645803361742346	0.0
27	2.0983610960495414E-5	2.0983610960495414E-5	0.0	3.2432059264432107	0.0
28	2.0983610960495414E-5	2.0983610960495414E-5	0.0	3.327413157227679	0.0
29	2.0983610960495414E-5	2.0983610960495414E-5	0.0	3.416992192418034	0.0
30	4.196722192099083E-5	2.0983610960495414E-5	0.0	3.5354446762900302	0.0
31	4.196722192099083E-5	2.0983610960495414E-5	0.0	3.6396912555417718	0.0
32	4.196722192099083E-5	2.0983610960495414E-5	0.0	3.7475470158787183	0.0
33	4.196722192099083E-5	2.0983610960495414E-5	0.0	3.851940480407183	0.0
34	4.196722192099083E-5	2.0983610960495414E-5	0.0	3.954990993834176	0.0
35	4.196722192099083E-5	2.0983610960495414E-5	0.0	4.08093462681907	0.0
36	4.196722192099083E-5	2.0983610960495414E-5	0.0	4.190469076032855	0.0
37	4.196722192099083E-5	2.0983610960495414E-5	0.0	4.304389099937385	0.0
38	4.196722192099083E-5	2.0983610960495414E-5	0.0	4.4230933871409075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCG	2400	0.0	38.866665	5
AGGGCGA	5740	0.0	38.86411	6
CGTAAGG	800	0.0	38.775	2
CGTTTTT	12245	0.0	38.430378	1
GGGCGAT	10095	0.0	37.941555	7
TATACGG	395	0.0	37.316456	2
GGCGATA	2430	0.0	36.395065	8
GCGATGT	1125	0.0	36.373333	9
AAGGGCG	2165	0.0	35.972286	5
CGGGATT	1820	0.0	35.9011	6
TACGGGT	420	0.0	35.61905	4
GCGATAT	635	0.0	35.338585	9
TTACGGG	1505	0.0	35.229233	3
CGACGGT	250	0.0	35.2	28
TATAGCG	225	0.0	35.2	1
CGCGTAG	25	0.0023534852	35.2	1
TACGGGA	1595	0.0	35.172413	4
GGCGATT	3205	0.0	35.076447	8
TAGGGAC	3650	0.0	34.9589	5
TAGTAGG	2645	0.0	34.767487	2
>>END_MODULE