FastQCFastQC Report
Thu 26 May 2016
SRR1547141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547141_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2719365
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326851.2019350105631277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAC207.858382E-444.035
CGTTTTT251450.042.740111
AGGGCGC2200.041.06
CGAACGC452.3530447E-839.1111131
ACGGGAT25350.038.3589745
ACGGGAC10500.037.9238135
TCGATAG1300.037.230771
GTTTTTT297700.036.949952
AAGGGAT73700.036.9253735
AGGGATT94100.036.822536
TACGGGA12550.036.812754
AGTACGG3050.036.7868842
TAGGGAC37200.036.4301075
TGGGCGA13800.036.3478286
TAGGGAT72400.036.3121575
AAGGGAC38450.036.2756845
GGCGATA11050.036.2352948
TTAGGGA46800.036.1965834
ATAGGGA48900.036.08184
GAGGGAT67650.036.0650375
Produced by FastQC (version 0.11.5)