##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547141_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2719365
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07954136351685	31.0	31.0	33.0	30.0	34.0
2	31.468639185986433	31.0	31.0	34.0	30.0	34.0
3	31.540704907211794	31.0	31.0	34.0	30.0	34.0
4	35.33825139324806	37.0	35.0	37.0	33.0	37.0
5	35.17467680874027	37.0	35.0	37.0	32.0	37.0
6	35.239204373079744	37.0	35.0	37.0	32.0	37.0
7	35.75602723429918	37.0	35.0	37.0	35.0	37.0
8	35.80924259891555	37.0	35.0	37.0	35.0	37.0
9	37.59862762078647	39.0	37.0	39.0	35.0	39.0
10	36.903185486317575	39.0	37.0	39.0	32.0	39.0
11	36.531987798622104	39.0	35.0	39.0	32.0	39.0
12	36.271804263127606	38.0	35.0	39.0	32.0	39.0
13	36.21961634425684	38.0	35.0	39.0	32.0	39.0
14	37.27066245244754	39.0	36.0	41.0	32.0	41.0
15	37.400850933949656	39.0	36.0	41.0	32.0	41.0
16	37.39061986897676	39.0	36.0	41.0	32.0	41.0
17	37.25857507175388	39.0	35.0	41.0	32.0	41.0
18	37.07916075995683	39.0	36.0	40.0	32.0	41.0
19	36.97474888439029	38.0	35.0	40.0	31.0	41.0
20	36.76934835889996	38.0	35.0	40.0	31.0	41.0
21	36.6649754630217	38.0	35.0	40.0	31.0	41.0
22	36.59927850803405	38.0	35.0	40.0	31.0	41.0
23	36.4070027377715	38.0	35.0	40.0	30.0	41.0
24	36.35260842145133	38.0	35.0	40.0	30.0	41.0
25	36.22862285864531	38.0	35.0	40.0	30.0	41.0
26	36.126971554020884	38.0	35.0	40.0	30.0	41.0
27	35.97284439565855	38.0	35.0	40.0	30.0	41.0
28	35.911942677794265	38.0	34.0	40.0	30.0	41.0
29	35.77606169087269	38.0	34.0	40.0	29.0	41.0
30	35.6832282536548	38.0	34.0	40.0	29.0	41.0
31	35.540822949475334	38.0	34.0	40.0	27.0	41.0
32	35.224215212007216	38.0	34.0	40.0	26.0	41.0
33	34.89107346751907	38.0	34.0	40.0	24.0	41.0
34	34.671678130740084	38.0	34.0	40.0	23.0	41.0
35	34.37955956629581	38.0	33.0	40.0	22.0	41.0
36	34.19898285077583	38.0	33.0	40.0	21.0	41.0
37	34.277430944356496	38.0	33.0	40.0	21.0	41.0
38	34.20205967201902	38.0	33.0	40.0	21.0	41.0
39	34.114296168406966	38.0	33.0	40.0	20.0	41.0
40	34.1369937467019	38.0	33.0	40.0	20.0	41.0
41	34.13175134636211	38.0	33.0	40.0	20.0	41.0
42	33.89442057244982	38.0	33.0	40.0	18.0	41.0
43	33.945558981600485	38.0	33.0	40.0	18.0	41.0
44	33.7541120813131	37.0	33.0	40.0	18.0	41.0
45	33.578967148580645	37.0	32.0	40.0	18.0	41.0
46	33.64651490329544	37.0	33.0	40.0	18.0	41.0
47	33.52210203485005	37.0	32.0	40.0	18.0	41.0
48	33.55040349493356	37.0	32.0	40.0	18.0	41.0
49	33.460318493471824	37.0	32.0	40.0	18.0	41.0
50	33.01521678774272	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	8.0
14	16.0
15	69.0
16	213.0
17	632.0
18	1545.0
19	3147.0
20	5720.0
21	9073.0
22	13668.0
23	19907.0
24	28925.0
25	42509.0
26	58603.0
27	69418.0
28	71208.0
29	73350.0
30	81015.0
31	93967.0
32	112961.0
33	139116.0
34	192010.0
35	239933.0
36	226161.0
37	277395.0
38	393972.0
39	564819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.56918251135835	21.877055856790093	27.827709777834166	20.726051854017392
2	35.54671770799433	23.210565701919382	28.796612444449348	12.44610414563694
3	26.765219086073404	24.33549744149829	36.52095250177891	12.378330970649397
4	24.134384313985066	27.107651970220992	35.30640425246335	13.451559463330593
5	21.65303296909389	30.812965526878518	34.307604900408734	13.22639660361886
6	19.70842457706119	39.2816337637647	31.174263109218515	9.835678549955597
7	84.48501764198627	3.0695769049024313	11.255715948392364	1.1896895047189324
8	85.59965286013463	2.403428741636375	10.830432840019636	1.1664855582093614
9	80.43392483171623	5.818122980916501	11.911346950482924	1.836605236884346
10	32.5966907715588	41.92228700450289	17.233287918319164	8.247734305619142
11	22.384674363316435	23.23568185955177	39.04470345098947	15.334940326142316
12	23.25120018827925	23.37041919712874	37.11528242806685	16.263098186525163
13	19.827349399584094	27.16950464538596	36.85235339867947	16.15079255635047
14	16.52378404517231	30.592399328519708	35.99656537463709	16.887251251670886
15	16.321383852480267	28.36710040763193	40.07299498228446	15.238520757603338
16	20.066265470063783	27.026052037883847	38.02236919280788	14.885313299244492
17	20.21876430710846	25.90093643185082	34.49422935133754	19.386069909703185
18	20.190522419756082	27.094965184886917	36.05606455918937	16.658447836167632
19	23.045637492576393	27.265960987215763	33.35602245377138	16.332379066436467
20	24.192522886776878	27.70018000525858	32.93871914950733	15.168577958457213
21	23.106644382052426	26.262822386843986	34.94609954897559	15.684433682127995
22	21.98248488158081	24.18469017583149	35.05196985325618	18.780855089331517
23	19.000869688327974	27.543452239769213	35.09194242038123	18.363735651521587
24	18.917504638031303	26.896242321277207	37.7393251733401	16.446927867351384
25	19.47447290084266	28.61068668604619	34.92171885715967	16.993121555951483
26	19.049962031577223	31.41292176666244	32.850573571403615	16.68654263035672
27	18.000820044385364	30.67870624208225	34.49996598470599	16.8205077288264
28	17.647539039444872	28.988569022547544	37.00510964875991	16.35878228924767
29	18.504797995120185	26.9808944367527	36.63517034307642	17.8791372250507
30	18.96942852467396	28.021983073254237	35.54995375758679	17.45863464448502
31	21.996973558165234	27.627368889428233	33.22014514417888	17.155512408227654
32	22.023744513884676	28.32072928790361	32.72852301916072	16.927003179050992
33	21.460892524541574	28.84000492762097	32.13150864264268	17.567593905194776
34	19.993344034360963	29.49107604164943	32.329238627400144	18.18634129658946
35	19.534744324502228	29.13665506469341	32.5099793517972	18.818621259007156
36	21.428384935453682	29.65673971680889	31.216920126573665	17.697955221163763
37	20.492467910707095	30.476379595971853	31.655625486096937	17.37552700722411
38	20.079319988306093	30.892616474802022	30.29523436537574	18.732829171516144
39	20.083070863970082	30.802264499248906	30.4689146179347	18.64575001884631
40	21.03329269884697	29.605404202819408	30.757364311153523	18.6039387871801
41	19.00105355478209	29.29209576500396	31.258142985586705	20.448707694627238
42	20.399063752015635	30.043741829434445	29.434555493653846	20.12263892489607
43	20.600287199401333	29.444888788375227	30.274383909478868	19.680440102744576
44	19.74685266597165	30.724305122703278	30.504621483324236	19.024220728000838
45	19.25692211233137	31.654081007882358	29.86605328817573	19.22294359161054
46	20.441242716590086	31.06559803483534	29.594813495062265	18.89834575351231
47	19.79969588488489	30.397795073482232	30.559229820196993	19.243279221435884
48	20.341145818968766	28.999968742702798	31.49202111522359	19.166864323104843
49	20.323752052409294	29.012508434873585	31.54799006385682	19.115749448860303
50	19.56703127384518	31.092699950172193	30.389852042664373	18.950416733318256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2449.0
1	2939.5
2	3430.0
3	29986.0
4	56542.0
5	41379.0
6	26216.0
7	26341.0
8	26466.0
9	27261.0
10	28056.0
11	27850.5
12	27645.0
13	26946.0
14	26247.0
15	25343.0
16	24439.0
17	23917.5
18	23396.0
19	23032.5
20	22669.0
21	23880.5
22	25092.0
23	28713.5
24	32335.0
25	37410.5
26	42486.0
27	48805.0
28	55124.0
29	65875.0
30	76626.0
31	87335.0
32	98044.0
33	107448.0
34	116852.0
35	127563.5
36	138275.0
37	142690.5
38	147106.0
39	157161.0
40	167216.0
41	183534.0
42	199852.0
43	207573.5
44	215295.0
45	213366.0
46	211437.0
47	195515.0
48	179593.0
49	167803.0
50	156013.0
51	143727.0
52	131441.0
53	118799.0
54	106157.0
55	94525.0
56	82893.0
57	76216.5
58	69540.0
59	64912.0
60	60284.0
61	54397.0
62	48510.0
63	42115.0
64	35720.0
65	27563.0
66	19406.0
67	16521.0
68	13636.0
69	11385.5
70	9135.0
71	7349.5
72	5564.0
73	4630.0
74	3696.0
75	2802.5
76	1909.0
77	1524.5
78	1140.0
79	990.0
80	840.0
81	607.0
82	374.0
83	271.5
84	169.0
85	99.0
86	29.0
87	21.5
88	14.0
89	7.5
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2719365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.375495372656285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81668264837015	17.635623178867252
2	5.662493477191385	2.534021931931844
3	2.1817133461319758	1.4645075064251545
4	1.302640525139503	1.1658890816997403
5	0.9259178891541068	1.0358935722113696
6	0.7342505217437655	0.9857531490988851
7	0.5739791080324678	0.8990146813047304
8	0.48780639736631765	0.8731927829617739
9	0.4447038961848374	0.8955422974157445
>10	6.9376714193297095	38.32151280187488
>50	1.6955682584548621	25.77359908359736
>100	0.2350690087985813	6.930021607047044
>500	0.0010023360682505152	0.14604213664107318
>1k	3.341120227501717E-4	0.11763363296889104
>5k	0.0	0.0
>10k+	1.6705601137508586E-4	1.2217525559543068
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32685	1.2019350105631277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.677329082340914E-5	0.0	0.0	0.012833878497369791	0.0
2	3.677329082340914E-5	0.0	0.0	0.049496849448308705	0.0
3	3.677329082340914E-5	0.0	0.0	0.08483598192960488	0.0
4	3.677329082340914E-5	0.0	0.0	0.12337439071253767	0.0
5	3.677329082340914E-5	0.0	0.0	0.20968130427507892	0.0
6	3.677329082340914E-5	0.0	0.0	0.42671726671483967	0.0
7	3.677329082340914E-5	0.0	0.0	0.5455317693652746	0.0
8	3.677329082340914E-5	0.0	0.0	0.7846684795899043	0.0
9	3.677329082340914E-5	0.0	0.0	0.9194425904576987	0.0
10	3.677329082340914E-5	0.0	0.0	1.0728239864821383	0.0
11	3.677329082340914E-5	0.0	0.0	1.1973383492102017	0.0
12	3.677329082340914E-5	0.0	0.0	1.3058931037209054	0.0
13	3.677329082340914E-5	0.0	0.0	1.3774539276632596	0.0
14	3.677329082340914E-5	0.0	0.0	1.4257004852235724	0.0
15	3.677329082340914E-5	0.0	0.0	1.4609292978323984	0.0
16	3.677329082340914E-5	0.0	0.0	1.5077416970505981	0.0
17	3.677329082340914E-5	0.0	0.0	1.5647770711177058	0.0
18	3.677329082340914E-5	0.0	0.0	1.6440235128421525	0.0
19	3.677329082340914E-5	0.0	0.0	1.6959841727756295	0.0
20	3.677329082340914E-5	0.0	0.0	1.7532034132968541	0.0
21	3.677329082340914E-5	0.0	0.0	1.8142470760637135	0.0
22	3.677329082340914E-5	0.0	0.0	1.8855137136794804	0.0
23	3.677329082340914E-5	0.0	0.0	1.9667091398175678	0.0
24	3.677329082340914E-5	0.0	0.0	2.0340042620244065	0.0
25	3.677329082340914E-5	0.0	0.0	2.0905615833108095	0.0
26	3.677329082340914E-5	0.0	0.0	2.145059600311102	0.0
27	3.677329082340914E-5	0.0	0.0	2.206728409021959	0.0
28	3.677329082340914E-5	0.0	0.0	2.2714861741619825	0.0
29	7.354658164681828E-5	0.0	0.0	2.3483791252737314	0.0
30	7.354658164681828E-5	0.0	0.0	2.4403858989139007	0.0
31	7.354658164681828E-5	0.0	0.0	2.5246335081903313	0.0
32	7.354658164681828E-5	0.0	0.0	2.6164196420855603	0.0
33	7.354658164681828E-5	0.0	0.0	2.6993801861831717	0.0
34	7.354658164681828E-5	0.0	0.0	2.7866064320162978	0.0
35	7.354658164681828E-5	0.0	0.0	2.8824008546112787	0.0
36	7.354658164681828E-5	0.0	0.0	2.980916500727192	0.0
37	7.354658164681828E-5	0.0	0.0	3.0777773487560514	0.0
38	7.354658164681828E-5	0.0	0.0	3.182360587857827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAC	20	7.858382E-4	44.0	35
CGTTTTT	25145	0.0	42.74011	1
AGGGCGC	220	0.0	41.0	6
CGAACGC	45	2.3530447E-8	39.11111	31
ACGGGAT	2535	0.0	38.358974	5
ACGGGAC	1050	0.0	37.923813	5
TCGATAG	130	0.0	37.23077	1
GTTTTTT	29770	0.0	36.94995	2
AAGGGAT	7370	0.0	36.925373	5
AGGGATT	9410	0.0	36.82253	6
TACGGGA	1255	0.0	36.81275	4
AGTACGG	305	0.0	36.786884	2
TAGGGAC	3720	0.0	36.430107	5
TGGGCGA	1380	0.0	36.347828	6
TAGGGAT	7240	0.0	36.312157	5
AAGGGAC	3845	0.0	36.275684	5
GGCGATA	1105	0.0	36.235294	8
TTAGGGA	4680	0.0	36.196583	4
ATAGGGA	4890	0.0	36.0818	4
GAGGGAT	6765	0.0	36.065037	5
>>END_MODULE