FastQCFastQC Report
Thu 26 May 2016
SRR1547140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547140_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences797484
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48070.6027707138952004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA650.041.53846431
GGCGATT5150.040.6310738
CGTTTTT29400.040.4081651
TAGCATA3600.040.030
TATAGCG403.4564437E-739.3750041
GGGCGAT18700.039.1042757
GGCGATA2950.038.8983048
AGGGCGA9900.038.6363646
CACGACG700.038.57142626
TCGTCAG356.245884E-638.5714261
TAGTGCG356.245884E-638.5714261
ACGGGAT1350.038.3333325
TTGATCC3950.038.1645617
CGTAAGG1300.038.0769272
GGTACCT3800.037.8947378
ATGGGCG2800.037.7678575
ATCCTGC4000.037.687520
TAGGGAT7150.037.447555
TGATCCT4150.037.4096418
GACACGA4150.037.4096425
Produced by FastQC (version 0.11.5)