FastQCFastQC Report
Thu 26 May 2016
SRR1547139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547139_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences657270
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131502.0006998645914162No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTT7740.11775982472956319No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG207.031081E-445.01
CGTTTTT71150.043.482081
GCGATCG750.042.0000049
TTCGTAG501.0804797E-940.51
ACAACGA451.9261279E-840.013
TAGGGCG3050.039.8360675
GCGCGAC2400.039.3750049
TACGGGA2300.039.1304324
ACGGGAC1400.038.5714265
CGATCAT356.244207E-638.57142610
GCGATGA1300.038.076929
GGGACCG5300.037.783027
TAGGGAT9750.037.6153835
GTTTTTT81550.037.6057622
TCGTTTG301.13938986E-437.5000041
CGCCAGT301.13938986E-437.50000426
GGGCGAT16000.037.406257
ATGGGCG2950.037.3728835
AGGGCGC2000.037.1256
ACGGGAT2850.037.1052635
Produced by FastQC (version 0.11.5)