##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547139_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	657270
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.484234789355973	31.0	31.0	33.0	30.0	34.0
2	31.83365587962329	31.0	31.0	34.0	30.0	34.0
3	31.803543444855233	31.0	31.0	34.0	30.0	34.0
4	35.64592785308929	37.0	35.0	37.0	33.0	37.0
5	35.618178222039646	37.0	35.0	37.0	33.0	37.0
6	35.663902201530576	37.0	35.0	37.0	33.0	37.0
7	36.05859996652821	37.0	35.0	37.0	35.0	37.0
8	34.77964306905838	37.0	35.0	37.0	35.0	37.0
9	37.08301459065529	39.0	37.0	39.0	34.0	39.0
10	36.99283095227228	39.0	37.0	39.0	32.0	39.0
11	36.861613948605594	39.0	37.0	39.0	32.0	39.0
12	36.70193527773974	39.0	35.0	39.0	32.0	39.0
13	36.66961218372967	39.0	35.0	39.0	33.0	39.0
14	37.828035662665265	40.0	36.0	41.0	33.0	41.0
15	37.955637713572806	40.0	36.0	41.0	33.0	41.0
16	37.95220685563011	40.0	36.0	41.0	33.0	41.0
17	37.86598962374671	40.0	36.0	41.0	33.0	41.0
18	37.68111126325559	39.0	36.0	41.0	33.0	41.0
19	37.50456889862613	39.0	36.0	41.0	33.0	41.0
20	37.262144932828214	39.0	35.0	41.0	32.0	41.0
21	37.18123602172623	39.0	35.0	41.0	32.0	41.0
22	37.1032832778006	39.0	35.0	41.0	32.0	41.0
23	37.01948818598141	39.0	35.0	41.0	32.0	41.0
24	36.87522022912958	38.0	35.0	41.0	32.0	41.0
25	36.78102606234881	38.0	35.0	41.0	32.0	41.0
26	36.692534270543305	38.0	35.0	41.0	31.0	41.0
27	36.57623351134237	38.0	35.0	40.0	31.0	41.0
28	36.54644057997474	38.0	35.0	40.0	31.0	41.0
29	36.475649276553014	38.0	35.0	40.0	31.0	41.0
30	36.36931854489023	38.0	35.0	40.0	31.0	41.0
31	36.19232278972112	38.0	35.0	40.0	30.0	41.0
32	35.91375994644515	38.0	35.0	41.0	30.0	41.0
33	35.532978836703336	38.0	35.0	41.0	27.0	41.0
34	35.144337943310965	38.0	35.0	41.0	24.0	41.0
35	34.87997017968263	38.0	34.0	41.0	23.0	41.0
36	34.69730704276781	38.0	34.0	41.0	21.0	41.0
37	34.63872837646629	38.0	34.0	40.0	21.0	41.0
38	34.5375675141114	38.0	34.0	40.0	21.0	41.0
39	34.43602324767599	38.0	34.0	40.0	21.0	41.0
40	34.31449784715566	38.0	34.0	40.0	20.0	41.0
41	34.215424407016904	38.0	34.0	40.0	18.0	41.0
42	34.14114290930668	38.0	33.0	40.0	18.0	41.0
43	34.06202930302615	37.0	33.0	40.0	18.0	41.0
44	33.93895050740183	37.0	33.0	40.0	18.0	41.0
45	33.82895917963698	37.0	33.0	40.0	18.0	41.0
46	33.74649687343101	37.0	33.0	40.0	18.0	41.0
47	33.685321100917434	37.0	33.0	40.0	18.0	41.0
48	33.59899584645579	36.0	33.0	40.0	18.0	41.0
49	33.524921265233466	36.0	33.0	40.0	18.0	41.0
50	33.442636968064875	36.0	33.0	40.0	18.0	41.0
51	33.09485751669786	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	5.0
14	10.0
15	24.0
16	58.0
17	140.0
18	340.0
19	654.0
20	1124.0
21	1869.0
22	2834.0
23	4205.0
24	6531.0
25	10442.0
26	14966.0
27	16687.0
28	16113.0
29	15667.0
30	16044.0
31	18284.0
32	21925.0
33	27717.0
34	42725.0
35	63643.0
36	53068.0
37	65348.0
38	92937.0
39	163792.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.19398420740335	22.842971685912943	26.261353781550962	20.701690325132745
2	36.758561930409115	24.079754134526148	26.904164194318923	12.257519740745812
3	26.54525537450363	23.57676449556499	37.0681759398725	12.809804190058879
4	23.157454318620964	26.657994431512165	36.40984679051227	13.774704459354602
5	20.913779725227073	30.465714242244434	35.181888721529965	13.438617310998524
6	19.587688468970136	38.117972826996514	32.656290413376546	9.638048290656807
7	81.51475040698647	3.136610525354877	13.837235839152859	1.5114032285057892
8	79.55862887397873	5.2967578012080265	13.014286366333469	2.130326958479772
9	76.7908165594048	5.007226862628753	15.39443455505348	2.8075220229129583
10	37.603268063352964	30.585908378596315	20.527789188613507	11.283034369437217
11	26.906294216988453	24.373545118444476	33.09735725044503	15.62280341412205
12	25.502761422246568	22.066578422870357	35.065802486040745	17.364857668842333
13	21.32533053387497	25.70130996394176	36.387481552482235	16.585877949701036
14	18.778127710073488	28.148706011228263	35.520258037031965	17.552908241666287
15	17.54317099517702	24.123571743727844	41.47884431055731	16.85441295053783
16	19.899584645579445	23.091271471389234	39.78334626561383	17.2257976174175
17	20.43726322515861	23.0775784685137	34.720282380148184	21.7648759261795
18	20.940861442025348	24.067734720890957	36.584204360460696	18.407199476623003
19	23.753404232659335	25.517367291980463	32.57245880688301	18.156769668477185
20	24.49647785537146	25.503826433581327	31.969510246930483	18.030185464116723
21	22.90869809971549	26.017618330366517	33.99029318240601	17.083390387511983
22	22.031128759870374	23.607649824273132	33.75918572276234	20.602035693094162
23	20.18759413939477	26.8407199476623	32.76233511342371	20.209350799519225
24	20.172836125184475	25.006313995770384	37.09601837905275	17.724831499992394
25	20.582104766686445	25.88342690218632	34.44474873339723	19.089719597730003
26	19.050922756249335	28.427738980936297	32.71745249288725	19.803885769927124
27	19.260577844721347	27.15444185798835	33.746862020174355	19.83811827711595
28	18.40628660976463	26.96000121715581	36.127466642323554	18.506245530756004
29	19.475710134343576	25.278196175087864	35.46168241361997	19.784411276948592
30	20.47164787682383	26.544342507645258	34.65896815616109	18.325041459369817
31	23.228505789097326	26.43434205121183	31.52357478661737	18.81357737307347
32	23.327703987706727	26.728589468559345	31.124347680557456	18.819358863176472
33	23.495823634122964	26.757649063550748	30.44015397021011	19.306373332116177
34	20.38416480289683	28.120863572048016	31.331568457407155	20.163403167648
35	21.00004564334292	27.317845025636345	31.838057419325395	19.844051911695345
36	22.734949107672648	28.842941257017664	29.016081671154932	19.406027964154763
37	20.879395073561856	29.626485310450807	30.08398375096992	19.41013586501742
38	21.19433413969906	30.271882179317476	29.02064600544677	19.51313767553669
39	20.799062790025406	29.24232050755397	28.56923334398345	21.389383358437172
40	22.043908895887533	27.78538500159752	29.74211511251084	20.428590990004107
41	19.328586425669815	27.312063535533344	30.496599570952576	22.862750467844265
42	21.04873187578925	28.731115066867495	28.273160192919196	21.946992864424058
43	20.668979262707868	27.70763917415978	29.926818506854108	21.696563056278244
44	21.090267317845026	28.412676677773213	29.31413270041231	21.18292330396945
45	20.060857790557915	29.049553455961778	29.303026153635493	21.586562599844815
46	21.216547233252697	29.120909215390935	29.036469030991828	20.626074520364536
47	20.895978821488885	28.167571926301214	29.950553045171695	20.985896207038206
48	21.069575669055336	27.156876169610662	30.72846775297823	21.045080408355773
49	20.729684908789388	26.711397142726735	30.940405008596162	21.61851293988772
50	20.39816209472515	28.07293806198366	30.844706132943845	20.684193710347344
51	19.287659561519618	28.870631551721516	30.018561626120167	21.823147260638702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	699.0
1	884.5
2	1070.0
3	10678.0
4	20286.0
5	13670.5
6	7055.0
7	7034.0
8	7013.0
9	7409.5
10	7806.0
11	8013.0
12	8220.0
13	8276.0
14	8332.0
15	8027.0
16	7722.0
17	7367.0
18	7012.0
19	6578.5
20	6145.0
21	5911.5
22	5678.0
23	5562.5
24	5447.0
25	5812.0
26	6720.0
27	7263.0
28	8322.0
29	9381.0
30	10753.0
31	12125.0
32	13382.0
33	14639.0
34	16149.0
35	17659.0
36	19835.0
37	22011.0
38	23866.5
39	25722.0
40	28950.0
41	32178.0
42	36119.5
43	40061.0
44	41757.0
45	43453.0
46	46410.5
47	49368.0
48	49809.5
49	50251.0
50	47178.5
51	44106.0
52	39923.0
53	35740.0
54	32934.5
55	30129.0
56	28199.0
57	26269.0
58	24561.0
59	22853.0
60	21480.0
61	20107.0
62	18400.5
63	16694.0
64	14388.5
65	12083.0
66	10540.5
67	8998.0
68	7533.0
69	6068.0
70	5223.5
71	4379.0
72	3612.0
73	2845.0
74	2356.0
75	1471.0
76	1075.0
77	878.0
78	681.0
79	489.5
80	298.0
81	232.0
82	166.0
83	125.5
84	85.0
85	57.0
86	29.0
87	24.0
88	19.0
89	11.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	657270.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.449661757533065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35558018816612	30.40563986827079
2	8.34557682894587	6.918426734626232
3	4.172230564378824	5.188126370038305
4	2.648535266029618	4.391235637193021
5	1.9631900208764252	4.068678116554605
6	1.5226998443190154	3.7869236103162867
7	1.2270581708671524	3.560280229748247
8	1.0357844005108394	3.434633044392276
9	0.8206801544960661	3.0615223333483725
>10	4.872251657140085	31.12155385836312
>50	0.02102188320867987	0.57649134563259
>100	0.013889458030921632	1.1427131755939992
>500	0.0011261722727774225	0.29765935524391524
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.753907575924742E-4	2.0461163206782462
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13150	2.0006998645914162	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTT	774	0.11775982472956319	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.521444763947845E-4	0.0	0.0	0.062227090845466855	0.0
2	1.521444763947845E-4	0.0	0.0	0.18272551615013616	0.0
3	1.521444763947845E-4	0.0	0.0	0.327567057677971	0.0
4	1.521444763947845E-4	0.0	0.0	0.5113575851628707	0.0
5	1.521444763947845E-4	0.0	0.0	0.8842636968064874	0.0
6	1.521444763947845E-4	0.0	0.0	1.6220122628447975	0.0
7	1.521444763947845E-4	0.0	0.0	2.005112054406865	0.0
8	1.521444763947845E-4	0.0	0.0	2.7040637789645046	0.0
9	1.521444763947845E-4	0.0	0.0	3.0908150379600468	0.0
10	1.521444763947845E-4	0.0	0.0	3.6015640452173385	0.0
11	1.521444763947845E-4	0.0	0.0	4.000182573371673	0.0
12	1.521444763947845E-4	0.0	0.0	4.318316673513168	0.0
13	1.521444763947845E-4	0.0	0.0	4.509105846912228	0.0
14	1.521444763947845E-4	0.0	0.0	4.614998402482998	0.0
15	1.521444763947845E-4	0.0	0.0	4.7026336208863935	0.0
16	1.521444763947845E-4	0.0	0.0	4.852952363564441	0.0
17	1.521444763947845E-4	0.0	0.0	5.032634990186681	0.0
18	1.521444763947845E-4	0.0	0.0	5.288237710529919	0.0
19	1.521444763947845E-4	0.0	0.0	5.413909048032012	0.0
20	1.521444763947845E-4	0.0	0.0	5.55251266602766	0.0
21	1.521444763947845E-4	0.0	0.0	5.716067978152053	0.0
22	1.521444763947845E-4	0.0	0.0	5.907617873933087	0.0
23	1.521444763947845E-4	0.0	0.0	6.115295084211968	0.0
24	1.521444763947845E-4	0.0	0.0	6.284175453010178	0.0
25	1.521444763947845E-4	0.0	0.0	6.430994872731145	0.0
26	1.521444763947845E-4	0.0	0.0	6.56914205729761	0.0
27	1.521444763947845E-4	0.0	0.0	6.718852222070078	0.0
28	1.521444763947845E-4	0.0	0.0	6.867801664460572	0.0
29	1.521444763947845E-4	0.0	0.0	7.025727630958358	0.0
30	1.521444763947845E-4	0.0	0.0	7.250445022593455	0.0
31	1.521444763947845E-4	0.0	0.0	7.432257671885222	0.0
32	1.521444763947845E-4	0.0	0.0	7.600225173825065	0.0
33	1.521444763947845E-4	0.0	0.0	7.7809728117820685	0.0
34	1.521444763947845E-4	0.0	0.0	7.962785461073835	0.0
35	1.521444763947845E-4	0.0	0.0	8.167876215254005	0.0
36	1.521444763947845E-4	0.0	0.0	8.353644620932037	0.0
37	1.521444763947845E-4	0.0	0.0	8.554171040820362	0.0
38	1.521444763947845E-4	0.0	0.0	8.755458183090663	0.0
39	1.521444763947845E-4	0.0	0.0	8.987478509592709	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGCG	20	7.031081E-4	45.0	1
CGTTTTT	7115	0.0	43.48208	1
GCGATCG	75	0.0	42.000004	9
TTCGTAG	50	1.0804797E-9	40.5	1
ACAACGA	45	1.9261279E-8	40.0	13
TAGGGCG	305	0.0	39.836067	5
GCGCGAC	240	0.0	39.375004	9
TACGGGA	230	0.0	39.130432	4
ACGGGAC	140	0.0	38.571426	5
CGATCAT	35	6.244207E-6	38.571426	10
GCGATGA	130	0.0	38.07692	9
GGGACCG	530	0.0	37.78302	7
TAGGGAT	975	0.0	37.615383	5
GTTTTTT	8155	0.0	37.605762	2
TCGTTTG	30	1.13938986E-4	37.500004	1
CGCCAGT	30	1.13938986E-4	37.500004	26
GGGCGAT	1600	0.0	37.40625	7
ATGGGCG	295	0.0	37.372883	5
AGGGCGC	200	0.0	37.125	6
ACGGGAT	285	0.0	37.105263	5
>>END_MODULE