FastQCFastQC Report
Thu 26 May 2016
SRR1547138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547138_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences760603
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.5180100525504107No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT12000.15776955915240934No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11110.14606831684860563No Hit
GAGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT8190.10767772412151938No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT8050.10583707926474127No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.031781E-445.01
ATTCGCG207.031781E-445.01
TTACGCG253.8895574E-545.01
TAATGCG406.8102963E-945.01
AATACGG750.045.02
GCGCGAC2800.043.3928579
CGGGTAC750.042.06
AATCGTT750.042.022
CTACGAA650.041.5384611
CGTTTTT23250.041.5161251
TACGCGG603.6379788E-1241.2499962
GCGTAAG603.6379788E-1241.2499961
AGTACGG850.039.7058832
TCGTTGA800.039.37524
TAATCGT800.039.37521
CGGTCTA1550.039.1935531
CGCGACC3150.038.5714310
TACGGGA2400.038.4374964
TAGGGAT4600.038.1521765
ACGGGAT1300.038.076925