##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547138_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 760603 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.404426487931286 31.0 31.0 33.0 30.0 34.0 2 31.760475570041137 31.0 31.0 34.0 30.0 34.0 3 31.888033573362186 31.0 31.0 34.0 30.0 34.0 4 35.62765989616133 37.0 35.0 37.0 33.0 37.0 5 35.55057631905212 37.0 35.0 37.0 33.0 37.0 6 35.60540387035024 37.0 35.0 37.0 33.0 37.0 7 35.97525384464694 37.0 35.0 37.0 35.0 37.0 8 34.654868571383496 37.0 35.0 37.0 33.0 37.0 9 36.977408713875704 39.0 37.0 39.0 33.0 39.0 10 36.851461274804336 39.0 37.0 39.0 32.0 39.0 11 36.76373088194498 39.0 35.0 39.0 32.0 39.0 12 36.25426799526166 38.0 35.0 39.0 32.0 39.0 13 36.042712163901534 38.0 35.0 39.0 32.0 39.0 14 37.04358909970116 39.0 35.0 41.0 31.0 41.0 15 37.31091252598267 39.0 35.0 41.0 32.0 41.0 16 37.42334437281998 39.0 35.0 41.0 33.0 41.0 17 37.34559816356233 39.0 35.0 41.0 32.0 41.0 18 37.292402212455116 39.0 35.0 41.0 32.0 41.0 19 37.21106280148777 39.0 35.0 41.0 32.0 41.0 20 37.07704544946575 39.0 35.0 41.0 32.0 41.0 21 36.96487392240104 39.0 35.0 41.0 32.0 41.0 22 36.88240514433942 38.0 35.0 41.0 32.0 41.0 23 36.8295536567697 38.0 35.0 41.0 32.0 41.0 24 36.691339634474225 38.0 35.0 41.0 31.0 41.0 25 36.57586283514527 38.0 35.0 40.0 31.0 41.0 26 36.456397095462414 38.0 35.0 40.0 31.0 41.0 27 36.325068399677626 38.0 35.0 40.0 31.0 41.0 28 36.36717840976173 38.0 35.0 40.0 31.0 41.0 29 36.3313081857421 38.0 35.0 40.0 31.0 41.0 30 36.28044722411035 38.0 35.0 40.0 31.0 41.0 31 36.16514002705748 38.0 35.0 40.0 30.0 41.0 32 36.030850522545926 38.0 35.0 40.0 30.0 41.0 33 35.9266056010823 38.0 35.0 40.0 30.0 41.0 34 35.77527172519698 38.0 35.0 40.0 30.0 41.0 35 35.67244935925838 38.0 35.0 40.0 29.0 41.0 36 35.517509134200104 38.0 35.0 40.0 28.0 41.0 37 35.46565948333099 38.0 35.0 40.0 28.0 41.0 38 35.36488680691504 38.0 34.0 40.0 28.0 41.0 39 35.27927841462629 37.0 34.0 40.0 27.0 41.0 40 35.107886768787395 37.0 34.0 40.0 26.0 41.0 41 35.025916279583434 37.0 34.0 40.0 26.0 41.0 42 34.96490810580553 37.0 34.0 40.0 26.0 41.0 43 34.868190107059796 37.0 34.0 40.0 26.0 41.0 44 34.73625794271124 37.0 34.0 40.0 26.0 41.0 45 34.6333382855445 36.0 34.0 40.0 25.0 41.0 46 34.573334577959855 36.0 34.0 40.0 25.0 41.0 47 34.51088149796937 36.0 34.0 40.0 25.0 41.0 48 34.424810314973776 36.0 34.0 40.0 24.0 41.0 49 34.375262784921965 36.0 34.0 40.0 24.0 41.0 50 34.2522432859192 35.0 34.0 40.0 24.0 41.0 51 33.866730738637635 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 0.0 12 4.0 13 3.0 14 8.0 15 27.0 16 67.0 17 170.0 18 386.0 19 757.0 20 1196.0 21 1986.0 22 2964.0 23 4242.0 24 6196.0 25 8398.0 26 10735.0 27 12732.0 28 14139.0 29 15965.0 30 18998.0 31 22505.0 32 27862.0 33 36329.0 34 62007.0 35 99982.0 36 57299.0 37 73303.0 38 105701.0 39 176505.0 40 135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.94780194135443 23.872506419248936 28.672382307195736 13.507309332200897 2 29.998566926504367 26.865789380268023 29.589154920503862 13.546488772723746 3 29.671589515160996 25.696191048418164 30.79372550463251 13.838493931788332 4 24.68791209080164 29.13280647065552 30.488572882305228 15.690708556237617 5 23.050921439962764 33.30528541170624 29.531306082147978 14.112487066183016 6 21.26589035278588 41.3061741802228 26.412464846970103 11.01547062002122 7 86.96954916033725 4.233220221324396 6.977490228147929 1.8197403901904146 8 84.64507765549176 6.222168463705771 6.017199511440265 3.1155543693622034 9 82.41710853099448 5.018649676638141 9.182714241200731 3.38152755116664 10 45.83021628891814 23.179766579937233 15.786290614157453 15.203726516987178 11 38.839973021405385 23.810581867281616 21.854765232322247 15.494679878990747 12 34.433732183543846 21.23473086485328 25.896032490011216 18.43550446159166 13 22.913398974234916 32.28688290737743 27.650692937051264 17.149025181336384 14 18.03634747693606 34.96252315596967 29.478847703729805 17.52228166336446 15 16.994411013367024 24.480445120516222 41.49562912583831 17.029514740278437 16 19.25393404969478 20.190690807162213 40.18061985030298 20.37475529284002 17 19.97599273208231 21.551453254851744 29.131623198961876 29.34093081410407 18 22.433122141248457 23.244452099189722 32.615832438210205 21.706593321351612 19 26.916670063094678 26.256272983409218 26.282173486036736 20.54488346745937 20 28.614664943472484 24.986228032232322 24.618362010141954 21.780745014153243 21 23.334775171804477 28.441775801567964 27.916271694957818 20.30717733166974 22 23.479660216959438 24.42469987628237 26.71025488987027 25.385385016887916 23 21.382245402660786 29.486736181687423 25.22787840700076 23.90314000865103 24 21.72171290410372 25.14662708403727 33.67039046651144 19.461269545347573 25 21.172280414355452 25.273894528420215 31.33881933150408 22.215005725720253 26 19.05803684708054 30.36958833977778 27.529341851136536 23.043032962005146 27 20.43825754039887 29.442034806594243 27.936124364484492 22.183583288522396 28 19.827952295744296 27.305309077140112 32.89298096378794 19.97375766332765 29 19.81388451005321 24.831745338895587 32.135292655958494 23.219077495092712 30 21.954160054588268 27.11388201203519 29.05720855689499 21.874749376481557 31 28.012905549938665 25.883805349176903 24.947443015607355 21.155846085277076 32 28.254292975441853 26.334500389822285 24.812550042532045 20.598656592203817 33 27.863550367274385 25.3220142439617 24.68975273565842 22.124682653105495 34 21.50754072755432 27.508437384548838 27.264420466393112 23.71960142150373 35 22.61876432251779 25.75653790479396 28.66646594872752 22.958231823960727 36 28.087714615903437 25.968343537956066 25.678704922278772 20.265236923861725 37 22.50188337411238 29.0840293819509 27.212882410403328 21.201204833533392 38 22.98137135930308 29.676059652670318 25.479652328481485 21.862916659545125 39 21.528839618039896 27.79307996418631 26.845805236108717 23.832275181665075 40 23.908793417853992 24.591409710453416 27.62400358662798 23.875793285064614 41 19.75656157022783 23.219208969725337 29.139117253021617 27.885112207025216 42 23.318077893460845 25.14715298256778 25.937578473921345 25.597190650050027 43 22.909980633786613 24.51475999963187 28.2900540755164 24.285205291065115 44 21.757868428076144 27.287428527102836 28.12702553105891 22.827677513762108 45 20.26287038047444 29.81292474523503 25.912729768354847 24.011475105935684 46 22.73091218414863 27.810829039590956 26.99739548752766 22.460863288732753 47 23.369615949450633 24.916809426205262 27.97635560206836 23.737219022275745 48 22.879741468282404 23.68213115120503 30.18473500630421 23.25339237420836 49 22.70987624292831 23.100750325728402 30.300695632281226 23.888677799062062 50 21.22066307916219 27.786769181820215 27.93204865087306 23.060519088144538 51 20.842279086461662 28.493708281455632 26.772179441837597 23.89183319024511 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 978.0 1 1248.0 2 1518.0 3 4080.5 4 6643.0 5 4689.5 6 2736.0 7 2900.5 8 3065.0 9 3366.5 10 3668.0 11 3889.5 12 4111.0 13 4155.0 14 4199.0 15 4060.0 16 3921.0 17 3886.0 18 3851.0 19 3674.5 20 3498.0 21 3656.5 22 3815.0 23 3636.5 24 3458.0 25 3655.5 26 4506.5 27 5160.0 28 5768.0 29 6376.0 30 7791.0 31 9206.0 32 10291.0 33 11376.0 34 14156.0 35 16936.0 36 17085.5 37 17235.0 38 20402.0 39 23569.0 40 31862.0 41 40155.0 42 47147.0 43 54139.0 44 56497.5 45 58856.0 46 62718.5 47 66581.0 48 68241.0 49 69901.0 50 67103.0 51 64305.0 52 57947.0 53 51589.0 54 46296.5 55 41004.0 56 39244.0 57 37484.0 58 35371.5 59 33259.0 60 30633.5 61 28008.0 62 24866.0 63 21724.0 64 19688.5 65 17653.0 66 15203.5 67 12754.0 68 10569.0 69 8384.0 70 7183.5 71 5983.0 72 4958.5 73 3934.0 74 3331.5 75 1998.5 76 1268.0 77 1131.0 78 994.0 79 689.0 80 384.0 81 315.5 82 247.0 83 158.5 84 70.0 85 41.0 86 12.0 87 9.5 88 7.0 89 6.5 90 6.0 91 3.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 760603.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.910665246291206 #Duplication Level Percentage of deduplicated Percentage of total 1 78.28411115104095 21.849616204401563 2 6.449390855888698 3.60013578442402 3 2.582740152569894 2.1625798744960014 4 1.5093117945509433 1.685035849999617 5 1.0279461119948576 1.4345329911557518 6 0.777293680947087 1.3016870236182974 7 0.6189884529065731 1.2093465650276534 8 0.5338929560704917 1.192104605938908 9 0.4499052186590913 1.130143855549801 >10 6.900009436437098 46.94795376782982 >50 0.8191602175846975 14.218043106696932 >100 0.04339283083129218 1.9390075634492452 >500 0.0024107128239606768 0.4886207552837955 >1k 0.001446427694376406 0.8411920521286164 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3940 0.5180100525504107 No Hit GAGAGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT 1200 0.15776955915240934 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1111 0.14606831684860563 No Hit GAGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT 819 0.10767772412151938 No Hit TAGAGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT 805 0.10583707926474127 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05495639643808925 0.0 2 0.0 0.0 0.0 0.18472185884094594 0.0 3 0.0 0.0 0.0 0.287140597657385 0.0 4 0.0 0.0 0.0 0.6396240877303928 0.0 5 0.0 0.0 0.0 1.0189284028593104 0.0 6 0.0 0.0 0.0 2.0406177730037878 0.0 7 0.0 0.0 0.0 2.4567349852682674 0.0 8 0.0 0.0 0.0 3.6075324446524664 0.0 9 0.0 0.0 0.0 4.118179917775765 0.0 10 0.0 0.0 0.0 4.855752606813279 0.0 11 0.0 0.0 0.0 5.420962052476785 0.0 12 0.0 0.0 0.0 5.914649297991199 0.0 13 0.0 0.0 0.0 6.157877368351164 0.0 14 0.0 0.0 0.0 6.263845922248532 0.0 15 0.0 0.0 0.0 6.359165030903112 0.0 16 0.0 0.0 0.0 6.532974495236017 0.0 17 0.0 0.0 0.0 6.712568843404509 0.0 18 0.0 0.0 0.0 6.990900640675885 0.0 19 0.0 0.0 0.0 7.112120251957986 0.0 20 0.0 0.0 0.0 7.241491290462962 0.0 21 0.0 0.0 0.0 7.425292826875518 0.0 22 0.0 0.0 0.0 7.610672058879599 0.0 23 0.0 0.0 0.0 7.8124856199620565 0.0 24 0.0 0.0 0.0 7.976828910745816 0.0 25 1.314746326270078E-4 0.0 0.0 8.10896091653596 0.0 26 1.314746326270078E-4 0.0 0.0 8.239120802836696 0.0 27 1.314746326270078E-4 0.0 0.0 8.379798659747594 0.0 28 1.314746326270078E-4 0.0 0.0 8.509695596783079 0.0 29 1.314746326270078E-4 0.0 0.0 8.661417322834646 0.0 30 1.314746326270078E-4 0.0 0.0 8.865334478039134 0.0 31 1.314746326270078E-4 0.0 0.0 9.033490533169077 0.0 32 1.314746326270078E-4 0.0 0.0 9.191391566954113 0.0 33 1.314746326270078E-4 0.0 0.0 9.349161126106523 0.0 34 1.314746326270078E-4 0.0 0.0 9.51626538417545 0.0 35 1.314746326270078E-4 0.0 0.0 9.711110789728675 0.0 36 1.314746326270078E-4 0.0 0.0 9.870326569839982 0.0 37 1.314746326270078E-4 0.0 0.0 10.03743082790891 0.0 38 1.314746326270078E-4 0.0 0.0 10.229515266176968 0.0 39 2.629492652540156E-4 0.0 0.0 10.524938765689853 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTAG 20 7.031781E-4 45.0 1 ATTCGCG 20 7.031781E-4 45.0 1 TTACGCG 25 3.8895574E-5 45.0 1 TAATGCG 40 6.8102963E-9 45.0 1 AATACGG 75 0.0 45.0 2 GCGCGAC 280 0.0 43.392857 9 CGGGTAC 75 0.0 42.0 6 AATCGTT 75 0.0 42.0 22 CTACGAA 65 0.0 41.53846 11 CGTTTTT 2325 0.0 41.516125 1 TACGCGG 60 3.6379788E-12 41.249996 2 GCGTAAG 60 3.6379788E-12 41.249996 1 AGTACGG 85 0.0 39.705883 2 TCGTTGA 80 0.0 39.375 24 TAATCGT 80 0.0 39.375 21 CGGTCTA 155 0.0 39.19355 31 CGCGACC 315 0.0 38.57143 10 TACGGGA 240 0.0 38.437496 4 TAGGGAT 460 0.0 38.152176 5 ACGGGAT 130 0.0 38.07692 5 >>END_MODULE