##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547137_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1101681
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46472163902255	31.0	31.0	33.0	30.0	34.0
2	31.816506774647106	31.0	31.0	34.0	30.0	34.0
3	31.928487465972456	33.0	31.0	34.0	30.0	34.0
4	35.65510705912147	37.0	35.0	37.0	33.0	37.0
5	35.571679097669836	37.0	35.0	37.0	33.0	37.0
6	35.62868289459472	37.0	35.0	37.0	33.0	37.0
7	35.97142730064329	37.0	35.0	37.0	35.0	37.0
8	34.61926728336061	37.0	35.0	37.0	33.0	37.0
9	36.967343541369964	39.0	37.0	39.0	33.0	39.0
10	36.95998206377345	39.0	37.0	39.0	32.0	39.0
11	36.80453960810797	39.0	35.0	39.0	33.0	39.0
12	35.83341275741344	37.0	35.0	39.0	32.0	39.0
13	35.35434849107863	37.0	35.0	39.0	30.0	39.0
14	36.32799603514992	38.0	35.0	41.0	31.0	41.0
15	36.737937751490676	38.0	35.0	41.0	32.0	41.0
16	36.92291507251191	38.0	35.0	41.0	32.0	41.0
17	36.84797958755756	38.0	35.0	41.0	32.0	41.0
18	36.817745790296826	38.0	35.0	40.0	32.0	41.0
19	36.70952117718287	37.0	35.0	40.0	32.0	41.0
20	36.52605881375825	37.0	35.0	40.0	32.0	41.0
21	36.3744042059362	37.0	35.0	40.0	32.0	41.0
22	36.272662413166785	36.0	35.0	40.0	31.0	41.0
23	36.23628436906872	36.0	35.0	40.0	31.0	41.0
24	36.065442718899575	36.0	35.0	40.0	31.0	41.0
25	35.958245626456296	35.0	35.0	40.0	31.0	41.0
26	35.81620541699458	35.0	35.0	40.0	31.0	41.0
27	35.687143556074766	35.0	35.0	40.0	31.0	41.0
28	35.73249788278095	36.0	35.0	40.0	31.0	41.0
29	35.72707616814668	36.0	35.0	40.0	31.0	41.0
30	35.70569429807721	36.0	35.0	40.0	31.0	41.0
31	35.517021714997355	36.0	35.0	40.0	30.0	41.0
32	35.30509829978006	35.0	34.0	40.0	30.0	41.0
33	35.15735317210699	35.0	34.0	40.0	29.0	41.0
34	35.02145357866751	35.0	34.0	40.0	29.0	41.0
35	34.890872221632215	35.0	34.0	40.0	28.0	41.0
36	34.66470330340634	35.0	34.0	40.0	26.0	41.0
37	34.51603050247758	35.0	34.0	40.0	25.0	41.0
38	34.409755637067356	35.0	34.0	40.0	25.0	41.0
39	34.30732943565334	35.0	34.0	40.0	24.0	41.0
40	34.07154884217845	35.0	33.0	40.0	23.0	41.0
41	33.991685433442164	35.0	34.0	40.0	23.0	41.0
42	33.89293543230754	35.0	33.0	40.0	23.0	41.0
43	33.76813433289673	35.0	33.0	40.0	23.0	41.0
44	33.63128255819969	35.0	33.0	40.0	23.0	41.0
45	33.50905570668824	35.0	33.0	39.0	23.0	41.0
46	33.47253333769031	35.0	33.0	39.0	23.0	41.0
47	33.40398536418437	35.0	33.0	39.0	22.0	41.0
48	33.3435513547025	35.0	33.0	39.0	22.0	41.0
49	33.3355444997236	35.0	33.0	39.0	22.0	41.0
50	33.16870128467315	35.0	33.0	39.0	21.0	41.0
51	32.77802376550018	35.0	33.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	4.0
14	14.0
15	58.0
16	117.0
17	361.0
18	772.0
19	1379.0
20	2391.0
21	3758.0
22	5580.0
23	8029.0
24	11730.0
25	15601.0
26	19202.0
27	21135.0
28	23219.0
29	26140.0
30	29945.0
31	34921.0
32	43024.0
33	56859.0
34	109291.0
35	209913.0
36	71378.0
37	86194.0
38	122721.0
39	197736.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.64667267566564	25.228809428500625	26.069706203519893	15.054811692313837
2	28.03316023422388	29.257108001317988	29.66838858072346	13.041343183734675
3	28.129104522997128	27.089965244022547	31.6582567912127	13.122673441767626
4	24.682734838850813	30.922290572316307	29.690718093531608	14.704256495301271
5	23.487016659087338	33.417477473061616	29.451538149428007	13.643967718423028
6	20.7074461663585	41.43867417156146	26.85005913690079	11.003820525179249
7	85.28276334074927	5.8981683445570905	7.121117637501237	1.6979506771923996
8	82.85928503804641	7.809338637954181	5.635025020854494	3.6963513031449216
9	81.5655348508325	6.651925557398195	8.78766176415859	2.994877827610715
10	56.14755995610344	20.636645272088742	11.839815699825994	11.375979071981817
11	50.30930006054384	20.06016260605384	17.24047160657214	12.390065726830182
12	43.661640710877286	20.62039737455761	20.779245534778216	14.93871637978689
13	20.180433355935158	45.35786675090158	21.807129286971456	12.654570606191811
14	13.870893661595327	46.80520041645449	25.19168434419764	14.132221577752544
15	12.006742423623535	23.77666493295246	50.60775306100405	13.608839582419957
16	13.394439951310769	17.62325028751517	49.715571022827845	19.266738738346216
17	13.944054585674074	19.708518164514047	26.848243729355413	39.49918352045646
18	19.03990356555119	24.11578306242914	34.032809860567625	22.811503511452045
19	27.99040738653022	26.556780047944912	23.333705491880135	22.11910707364473
20	29.848477009224993	24.40987908478044	22.98405799864026	22.757585907354308
21	20.969954097420214	30.89151941442214	26.484345286884313	21.654181201273325
22	22.753410469999935	25.991552908691357	23.38952927390052	27.865507347408187
23	19.220173534807262	33.01273236081951	21.614060694520465	26.15303340985276
24	20.428054945124767	25.698455360490012	37.10157477527524	16.771914919109978
25	17.296113847837987	26.347735869094592	33.99650170966005	22.359648573407366
26	16.81966013755343	36.304429322099594	25.096193907310738	21.77971663303624
27	18.78974040579805	34.56953510135874	25.710255509534974	20.930468983308234
28	14.800926947092671	30.517273148942387	37.98531516836544	16.69648473559951
29	14.168438958282842	26.170733633420202	37.41373410270305	22.247093305593907
30	18.187842034127847	32.01589207765224	30.798025925835155	18.998239962384755
31	28.070376088904137	28.262446207205173	22.6632754853719	21.003902218518792
32	29.950593683652528	27.845719405163567	24.94751202934425	17.256174881839662
33	27.690411289656442	29.072934905839347	22.27532289292454	20.96133091157967
34	19.639169596280592	27.477736295715367	27.922601914710338	24.960492193293703
35	18.96293028562715	26.310610784791606	29.83631377867096	24.89014515091029
36	32.67424962398371	22.878582820253776	25.175164135534693	19.272003420227815
37	21.085504787683547	30.01676528868157	28.301659010185343	20.596070913449537
38	21.834269629774862	31.436958611431077	24.060957754558714	22.667814004235346
39	21.132069991222505	30.924832142879836	25.519819258024786	22.42327860787288
40	24.80463945552297	24.427760849102416	24.66939159339228	26.098208101982333
41	17.744519511546446	22.274687500283658	26.834174320878727	33.146618667291165
42	23.094616318153804	26.66388909312224	23.060214345168884	27.18128024355508
43	23.12366283887986	26.15639191381171	25.572556847218024	25.14738840009041
44	21.511762479338394	30.230983379036218	26.104017406127543	22.153236735497845
45	17.613537857147396	35.475968088766166	21.895358093676844	25.01513596040959
46	23.42810668423981	30.455458522022255	25.955153987406515	20.161280806331416
47	23.87896314813453	24.857740126225288	27.09840688910855	24.16488983653163
48	24.615201678162734	21.082963217120017	30.35561110702644	23.9462239976908
49	21.529916554792177	19.496206252082047	32.97687806179829	25.99699913132749
50	19.431668513843846	30.58544170227135	27.434348055380823	22.548541728503988
51	18.37074434432472	32.85343034871256	23.87224614021663	24.90357916674609
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2175.0
1	2351.5
2	2528.0
3	5137.0
4	7746.0
5	6069.0
6	4392.0
7	5110.5
8	5829.0
9	6688.5
10	7548.0
11	7922.0
12	8296.0
13	8524.0
14	8752.0
15	8716.5
16	8681.0
17	8342.0
18	8003.0
19	7646.5
20	7290.0
21	7103.5
22	6917.0
23	6736.5
24	6556.0
25	5958.0
26	5421.0
27	5482.0
28	6489.5
29	7497.0
30	8121.0
31	8745.0
32	9545.5
33	10346.0
34	13118.0
35	15890.0
36	16961.5
37	18033.0
38	25175.0
39	32317.0
40	49763.5
41	67210.0
42	84585.5
43	101961.0
44	105905.5
45	109850.0
46	109349.0
47	108848.0
48	107330.0
49	105812.0
50	96977.5
51	88143.0
52	77039.5
53	65936.0
54	59528.5
55	53121.0
56	48210.5
57	43300.0
58	40914.5
59	38529.0
60	35729.0
61	32929.0
62	29826.0
63	26723.0
64	24295.5
65	21868.0
66	18278.0
67	14688.0
68	13432.5
69	12177.0
70	10430.0
71	8683.0
72	7246.0
73	5809.0
74	4492.0
75	2513.0
76	1851.0
77	1521.5
78	1192.0
79	996.5
80	801.0
81	572.5
82	344.0
83	240.0
84	136.0
85	133.5
86	131.0
87	99.0
88	67.0
89	38.5
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1101681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.746171954134038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0380246841618	19.11543360151705
2	6.42752761246043	2.924032966259391
3	1.9942249777406396	1.3608295277675315
4	0.9851562971262674	0.8963413814452817
5	0.5952428458842896	0.676974806347608
6	0.4486645041088029	0.6123239976105065
7	0.3127071165429438	0.4979022890917063
8	0.2407898377163168	0.4381637642802697
9	0.1907109300834546	0.39041492482899837
>10	2.339545626945	12.998828071424217
>50	1.2778850325305504	21.714615737907554
>100	1.143349164613432	36.22031782178791
>500	0.0028799727068348413	0.46894623423276066
>1k	0.0032913973792398185	1.6848748754992264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4194	0.3806909622658465	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3345	0.30362691196453423	No Hit
GAGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	1973	0.17908995435157726	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	1844	0.16738057568388673	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	1831	0.16620056077939077	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1706	0.1548542636207759	No Hit
AAGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	1634	0.14831879645741372	No Hit
TAGAGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	1477	0.13406784722619344	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09276732556883527	0.0
2	0.0	0.0	0.0	0.2821143325518004	0.0
3	0.0	0.0	0.0	0.41109903865093433	0.0
4	0.0	0.0	0.0	0.9185054475841918	0.0
5	0.0	0.0	0.0	1.330875271516891	0.0
6	0.0	0.0	0.0	2.4527971345607305	0.0
7	0.0	0.0	0.0	2.809615487604851	0.0
8	0.0	0.0	0.0	3.786395517395689	0.0
9	0.0	0.0	0.0	4.241881270531125	0.0
10	0.0	0.0	0.0	4.9429916645562555	0.0
11	0.0	0.0	0.0	5.514209648709563	0.0
12	0.0	0.0	0.0	6.045397896487277	0.0
13	0.0	0.0	0.0	6.326786066020926	0.0
14	0.0	0.0	0.0	6.442064445152454	0.0
15	0.0	0.0	0.0	6.542547252789147	0.0
16	0.0	0.0	0.0	6.733165045053877	0.0
17	0.0	0.0	0.0	6.917428910909782	0.0
18	0.0	0.0	0.0	7.226502045510451	0.0
19	0.0	0.0	0.0	7.341780424641979	0.0
20	0.0	0.0	0.0	7.470946671495651	0.0
21	0.0	0.0	0.0	7.626527098134578	0.0
22	0.0	0.0	0.0	7.767130412524134	0.0
23	0.0	0.0	0.0	7.938232573676046	0.0
24	0.0	0.0	0.0	8.070031161470517	0.0
25	0.0	0.0	0.0	8.16715546514826	0.0
26	0.0	0.0	0.0	8.261375116753397	0.0
27	0.0	0.0	0.0	8.349150071572442	0.0
28	0.0	0.0	0.0	8.438558893182329	0.0
29	0.0	0.0	0.0	8.527876944414944	0.0
30	0.0	0.0	0.0	8.682821978412989	0.0
31	0.0	0.0	0.0	8.79692034264002	0.0
32	0.0	0.0	0.0	8.88269834915915	0.0
33	0.0	0.0	0.0	8.979459571327816	0.0
34	0.0	0.0	0.0	9.072589978405727	0.0
35	0.0	0.0	0.0	9.207111677518265	0.0
36	0.0	0.0	0.0	9.308683729682185	0.0
37	0.0	0.0	0.0	9.4099834707143	0.0
38	0.0	0.0	0.0	9.51409709344175	0.0
39	0.0	0.0	0.0	9.61657684937836	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	30	2.1654196E-6	45.000004	6
AGGTACA	35	1.2118653E-7	45.000004	17
GTACCCG	35	1.2118653E-7	45.000004	25
TACCCCG	30	2.1654196E-6	45.000004	25
GCCCACG	30	2.1654196E-6	45.000004	20
CACGGTA	30	2.1654196E-6	45.000004	23
CTATCGA	30	2.1654196E-6	45.000004	30
CGCATCA	30	2.1654196E-6	45.000004	36
GGCGTAG	45	3.8562575E-10	45.000004	1
ACATTCG	30	2.1654196E-6	45.000004	14
TCGATTG	30	2.1654196E-6	45.000004	1
CGTTCGA	30	2.1654196E-6	45.000004	14
TGCATAA	30	2.1654196E-6	45.000004	22
TAACCGG	45	3.8562575E-10	45.000004	2
GTACTAA	35	1.2118653E-7	45.000004	9
TTGCGAT	30	2.1654196E-6	45.000004	11
ACTCGAT	30	2.1654196E-6	45.000004	19
ACGTAAG	30	2.1654196E-6	45.000004	1
CGACTCC	30	2.1654196E-6	45.000004	28
TTCGAAT	30	2.1654196E-6	45.000004	16
>>END_MODULE