FastQCFastQC Report
Thu 26 May 2016
SRR1547136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547136_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1251194
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125991.0069581535717083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG302.165656E-645.0000041
CGCATCG302.165656E-645.00000421
CGGTAAT207.0335256E-445.0000046
CGTTTTT98400.042.804881
TACGGGT2000.040.5000044
ATACGTC403.4585537E-739.37500435
AGGGCGA14750.039.2033926
AGGGTAC8000.038.8125046
TCGTAAG700.038.5714261
GGGCGAT31400.038.1926737
TAGGGTA9500.037.894745
TACTCGC301.13997965E-437.50000445
CGTTAGG1700.037.0588232
CACGACC15950.036.95924827
TACGCGG1100.036.818182
CGAGGGA7150.036.818184
CGTAAGG2200.036.818182
ATAGGGT8450.036.745564
GCGAGAC16300.036.71779321
GGCGATT7050.036.7021268
Produced by FastQC (version 0.11.5)