##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547136_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1251194
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.438175055187283	31.0	31.0	33.0	30.0	34.0
2	31.81948442847392	31.0	31.0	34.0	30.0	34.0
3	31.854469410818787	31.0	31.0	34.0	30.0	34.0
4	35.662919579217935	37.0	35.0	37.0	33.0	37.0
5	35.62143680356523	37.0	35.0	37.0	33.0	37.0
6	35.668013913110194	37.0	35.0	37.0	33.0	37.0
7	36.041274174908125	37.0	35.0	37.0	35.0	37.0
8	34.728808641985175	37.0	35.0	37.0	33.0	37.0
9	37.03161300325929	39.0	37.0	39.0	34.0	39.0
10	36.75438660991021	39.0	37.0	39.0	32.0	39.0
11	36.837799733694375	39.0	37.0	39.0	32.0	39.0
12	36.752209489495634	39.0	35.0	39.0	32.0	39.0
13	36.775780574395334	39.0	35.0	39.0	33.0	39.0
14	37.90922191123039	40.0	37.0	41.0	33.0	41.0
15	38.005616235371974	40.0	37.0	41.0	33.0	41.0
16	37.998780364995355	40.0	37.0	41.0	33.0	41.0
17	37.85809954331622	40.0	36.0	41.0	33.0	41.0
18	37.74882312415181	39.0	36.0	41.0	33.0	41.0
19	37.637784388352244	39.0	36.0	41.0	33.0	41.0
20	37.45301927598758	39.0	35.0	41.0	32.0	41.0
21	37.36742903178884	39.0	35.0	41.0	32.0	41.0
22	37.26709367212439	39.0	35.0	41.0	32.0	41.0
23	37.16518061947228	39.0	35.0	41.0	32.0	41.0
24	37.02019031421187	39.0	35.0	41.0	32.0	41.0
25	36.92283370924093	39.0	35.0	41.0	31.0	41.0
26	36.83710839406199	39.0	35.0	41.0	31.0	41.0
27	36.7464597816166	38.0	35.0	41.0	31.0	41.0
28	36.760499171191675	39.0	35.0	41.0	31.0	41.0
29	36.701057549828406	39.0	35.0	41.0	31.0	41.0
30	36.60752768955094	38.0	35.0	41.0	31.0	41.0
31	36.47655919066108	39.0	35.0	41.0	30.0	41.0
32	36.27054157868404	39.0	35.0	41.0	30.0	41.0
33	35.99259027776668	39.0	35.0	41.0	29.0	41.0
34	35.67963880900963	39.0	35.0	41.0	26.0	41.0
35	35.46554251379083	39.0	35.0	41.0	25.0	41.0
36	35.31882745601401	39.0	35.0	41.0	24.0	41.0
37	35.282033801312984	39.0	35.0	41.0	24.0	41.0
38	35.15115241920917	38.0	35.0	41.0	23.0	41.0
39	35.06208229898801	38.0	34.0	41.0	23.0	41.0
40	34.93791929948513	38.0	34.0	40.0	23.0	41.0
41	34.82548429739912	38.0	34.0	40.0	23.0	41.0
42	34.75603543495254	38.0	34.0	40.0	23.0	41.0
43	34.66397537072588	38.0	34.0	40.0	22.0	41.0
44	34.541886390120155	38.0	34.0	40.0	22.0	41.0
45	34.413935009279136	38.0	34.0	40.0	22.0	41.0
46	34.32993284814345	37.0	34.0	40.0	22.0	41.0
47	34.263404396120826	37.0	33.0	40.0	22.0	41.0
48	34.1821436164176	37.0	33.0	40.0	21.0	41.0
49	34.095758131832476	37.0	33.0	40.0	20.0	41.0
50	34.00707963753023	37.0	33.0	40.0	20.0	41.0
51	33.679080941884315	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	1.0
12	4.0
13	3.0
14	10.0
15	36.0
16	99.0
17	276.0
18	638.0
19	1193.0
20	2060.0
21	3227.0
22	4795.0
23	7181.0
24	10934.0
25	16972.0
26	24151.0
27	27917.0
28	27844.0
29	27420.0
30	29797.0
31	33920.0
32	40873.0
33	50693.0
34	77624.0
35	105651.0
36	100064.0
37	130341.0
38	194319.0
39	332958.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.4876078369941	23.602574820531427	25.87608316536045	18.03373417711402
2	36.714849975303586	23.769455416186457	25.78409103624218	13.731603572267769
3	28.965452200058508	24.444490622557332	33.47762217529816	13.112435002086006
4	24.919476915650172	26.844438192638393	33.17766869086648	15.058416200844954
5	22.538870870544457	30.31272528480795	32.347341819094396	14.801062025553192
6	20.97636337770162	39.31708432105653	29.94955218775026	9.757000113491593
7	84.75935786137082	3.0343016350781733	10.769233228420214	1.437107275130795
8	82.33623243078212	5.4767685906422185	10.2322261775552	1.9547728010204652
9	79.68580411990467	4.143721916825049	13.263011171728763	2.907462791541519
10	31.729771722051098	26.689226450894104	28.842209921083384	12.738791905971416
11	29.22552377968564	27.032818252005686	27.566468509279936	16.175189459028736
12	25.22982047548182	24.42874566214352	31.767575611775634	18.57385825059903
13	22.66906650767187	26.18850474027209	33.09742533931588	18.04500341274015
14	21.16849984894429	26.91716872043824	33.70852162014843	18.205809810469038
15	21.618150342792564	24.170672173939455	35.890277606829954	18.320899876438027
16	23.641018099511346	23.480931014694765	34.94717845513965	17.930872430654237
17	22.56348735687671	23.530243911016196	32.46498944208492	21.441279290022173
18	24.28440353774075	22.90356251708368	33.10581732329279	19.706216621882778
19	25.609617693179477	25.631836469804043	29.794580216976748	18.963965620039737
20	27.926844278345325	24.487249779011087	29.394322543106828	18.19158339953676
21	26.045041776095474	25.545518920327304	30.32679184842638	18.08264745515084
22	24.50763031152643	22.30013890731573	31.43661174845787	21.755619032699965
23	22.931695644320545	26.480226088040702	30.790828600520783	19.79724966711797
24	21.669141635909376	25.31437970450626	34.2474468387796	18.76903182080477
25	22.788552374771616	25.68762318233623	31.986966050029014	19.536858392863138
26	21.682249115644737	26.7991214791631	30.83462676451453	20.684002640677623
27	21.3321834983224	25.209120248338785	31.528923572203833	21.929772681134978
28	20.285343439946164	27.10059351307631	32.534123405323236	20.079939641654292
29	21.137729241028968	25.195613150318813	32.12003893880565	21.546618669846563
30	22.458787366307703	24.50739054055566	33.07648534120208	19.95733675193455
31	24.09386553963654	25.561503651711888	30.165266137785185	20.179364670866388
32	23.389498351174957	25.008192174834598	30.332866046352525	21.26944342763792
33	22.86368061227915	25.406132062653754	29.74638625185223	21.983801073214863
34	20.763686526629762	26.934991695932048	31.304577867221226	20.99674391021696
35	21.34329288663469	27.444345161501733	30.76533295396238	20.447028997901203
36	23.098336469004806	28.768600233057384	27.80216337354559	20.33089992439222
37	22.344336689594098	28.374097062485916	28.453940795751897	20.82762545216809
38	22.081867400259274	28.145115785401785	29.323909801357743	20.449107012981198
39	22.68329291860415	27.182195566794597	27.75372963745031	22.380781877150945
40	23.781204193754128	26.31997915591027	29.27187950070093	20.62693714963467
41	20.9703691034324	27.146789386777748	28.92868731787397	22.954154191915883
42	22.802539014733128	27.317746088935845	27.627450259512116	22.25226463681891
43	22.20526952654824	26.03688956308934	29.185561951224194	22.57227895913823
44	22.51257598741682	26.859943382081436	29.085497532756712	21.541983097745035
45	22.000505117511754	26.706250189818686	28.321747067201407	22.971497625468153
46	22.613040024168914	26.495251735542208	28.540418192542482	22.351290047746392
47	21.68424720706781	27.451937908909407	29.703067629800017	21.160747254222766
48	22.57835315706437	26.68690866484334	29.54457901812189	21.190159159970396
49	22.251865018534296	26.87808605220294	29.13041462794738	21.739634301315384
50	21.183525496445796	27.311432120038937	30.0110934035809	21.493948979934366
51	21.664346216494003	26.823178499896898	29.294737666580883	22.217737617028217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	765.0
1	1271.0
2	1777.0
3	12492.5
4	23208.0
5	16700.5
6	10193.0
7	10103.5
8	10014.0
9	10331.5
10	10649.0
11	10599.5
12	10550.0
13	10399.5
14	10249.0
15	9869.5
16	9490.0
17	9022.5
18	8555.0
19	8471.5
20	8388.0
21	8320.0
22	8252.0
23	8466.5
24	8681.0
25	9228.0
26	11319.5
27	12864.0
28	14566.5
29	16269.0
30	18593.0
31	20917.0
32	24803.5
33	28690.0
34	32807.5
35	36925.0
36	40432.5
37	43940.0
38	48032.5
39	52125.0
40	56081.5
41	60038.0
42	64517.5
43	68997.0
44	71788.5
45	74580.0
46	79582.0
47	84584.0
48	87692.5
49	90801.0
50	91166.0
51	91531.0
52	87397.0
53	83263.0
54	77455.0
55	71647.0
56	66032.0
57	60417.0
58	55862.0
59	51307.0
60	48281.5
61	45256.0
62	40090.5
63	34925.0
64	30965.0
65	27005.0
66	23198.0
67	19391.0
68	16865.0
69	14339.0
70	12440.0
71	10541.0
72	9050.5
73	7560.0
74	6677.0
75	4823.0
76	3852.0
77	2809.5
78	1767.0
79	1224.0
80	681.0
81	546.0
82	411.0
83	281.0
84	151.0
85	100.5
86	50.0
87	34.5
88	19.0
89	13.0
90	7.0
91	4.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1251194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.84253695120757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.66237364999091	23.913939913098538
2	7.025385986634671	4.755137696983593
3	3.7208843260824174	3.7777249588983985
4	2.6836822414838917	3.6329046169087262
5	2.1427239649850325	3.6257607480621985
6	1.7956323306700221	3.6461252100889916
7	1.550612951181457	3.673367326816564
8	1.3216784372730976	3.5783161080823267
9	1.229026785466623	3.7434045961060245
>10	7.800019223441478	41.8508812980529
>50	0.043155632508350805	0.9953663454970504
>100	0.02410142752138786	1.668174369412757
>500	4.820285071323575E-4	0.11125512078758641
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.4101425356617875E-4	1.0276416912043995
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12599	1.0069581535717083	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5984731384581448E-4	0.0	0.0	0.03180961545531708	0.0
2	1.5984731384581448E-4	0.0	0.0	0.1027818228028587	0.0
3	1.5984731384581448E-4	0.0	0.0	0.18262555606884304	0.0
4	1.5984731384581448E-4	0.0	0.0	0.2937194391916841	0.0
5	1.5984731384581448E-4	0.0	0.0	0.5052773590666195	0.0
6	1.5984731384581448E-4	0.0	0.0	0.985698460830215	0.0
7	1.5984731384581448E-4	0.0	0.0	1.2265883627958574	0.0
8	1.5984731384581448E-4	0.0	0.0	1.761996940522413	0.0
9	1.5984731384581448E-4	0.0	0.0	2.0580341657648615	0.0
10	1.5984731384581448E-4	0.0	0.0	2.464206190247076	0.0
11	1.5984731384581448E-4	0.0	0.0	2.7404223485726433	0.0
12	1.5984731384581448E-4	0.0	0.0	2.960771870709099	0.0
13	1.5984731384581448E-4	0.0	0.0	3.0964023165072723	0.0
14	1.5984731384581448E-4	0.0	0.0	3.1781642175394063	0.0
15	1.5984731384581448E-4	0.0	0.0	3.249056501230025	0.0
16	1.5984731384581448E-4	0.0	0.0	3.36190870480517	0.0
17	1.5984731384581448E-4	0.0	0.0	3.4777980073433854	0.0
18	1.5984731384581448E-4	0.0	0.0	3.641321809407654	0.0
19	1.5984731384581448E-4	0.0	0.0	3.7231636340967107	0.0
20	1.5984731384581448E-4	0.0	0.0	3.818432633148816	0.0
21	1.5984731384581448E-4	0.0	0.0	3.928247737760891	0.0
22	1.5984731384581448E-4	0.0	0.0	4.053967650100624	0.0
23	2.3977097076872172E-4	0.0	0.0	4.1992688583864695	0.0
24	2.3977097076872172E-4	0.0	0.0	4.316436939435452	0.0
25	2.3977097076872172E-4	0.0	0.0	4.422016090230612	0.0
26	2.3977097076872172E-4	0.0	0.0	4.516885470998103	0.0
27	2.3977097076872172E-4	0.0	0.0	4.621665385224034	0.0
28	2.3977097076872172E-4	0.0	0.0	4.734917207083794	0.0
29	2.3977097076872172E-4	0.0	0.0	4.854802692468154	0.0
30	2.3977097076872172E-4	0.0	0.0	5.013131456832434	0.0
31	2.3977097076872172E-4	0.0	0.0	5.13949075842755	0.0
32	2.3977097076872172E-4	0.0	0.0	5.278238226845717	0.0
33	2.3977097076872172E-4	0.0	0.0	5.416426229665424	0.0
34	2.3977097076872172E-4	0.0	0.0	5.547900645303606	0.0
35	2.3977097076872172E-4	0.0	0.0	5.700554830026359	0.0
36	2.3977097076872172E-4	0.0	0.0	5.83474664999992	0.0
37	2.3977097076872172E-4	0.0	0.0	5.974213431330393	0.0
38	2.3977097076872172E-4	0.0	0.0	6.151324255071556	0.0
39	2.3977097076872172E-4	0.0	0.0	6.380385455812608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	30	2.165656E-6	45.000004	1
CGCATCG	30	2.165656E-6	45.000004	21
CGGTAAT	20	7.0335256E-4	45.000004	6
CGTTTTT	9840	0.0	42.80488	1
TACGGGT	200	0.0	40.500004	4
ATACGTC	40	3.4585537E-7	39.375004	35
AGGGCGA	1475	0.0	39.203392	6
AGGGTAC	800	0.0	38.812504	6
TCGTAAG	70	0.0	38.571426	1
GGGCGAT	3140	0.0	38.192673	7
TAGGGTA	950	0.0	37.89474	5
TACTCGC	30	1.13997965E-4	37.500004	45
CGTTAGG	170	0.0	37.058823	2
CACGACC	1595	0.0	36.959248	27
TACGCGG	110	0.0	36.81818	2
CGAGGGA	715	0.0	36.81818	4
CGTAAGG	220	0.0	36.81818	2
ATAGGGT	845	0.0	36.74556	4
GCGAGAC	1630	0.0	36.717793	21
GGCGATT	705	0.0	36.702126	8
>>END_MODULE