FastQCFastQC Report
Thu 26 May 2016
SRR1547135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547135_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences517143
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34260.6624860048381203No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11340.21928170738074382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6590.12743090402461216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA351.2101918E-745.0000045
TATAGCG351.2101918E-745.0000041
GCGACGT207.0296845E-445.017
CATATCC253.8878185E-545.029
AGTTTAG502.1827873E-1145.01
CGAGTCT207.0296845E-445.017
ACCATCG207.0296845E-445.017
CGTGAAC207.0296845E-445.034
CGACAGG253.8878185E-545.02
TAACGCC253.8878185E-545.012
TCTAGCG207.0296845E-445.01
CTAACGC207.0296845E-445.033
CTAAACG207.0296845E-445.023
TGATTCG207.0296845E-445.015
ATTGGGC551.8189894E-1245.04
TTCGGTA207.0296845E-445.035
ATAACTC207.0296845E-445.011
CGCACGG253.8878185E-545.02
CCACGTT207.0296845E-445.016
TCGACAC207.0296845E-445.038