##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547135_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	517143
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.630560599292654	31.0	31.0	33.0	30.0	34.0
2	31.996668232964577	33.0	31.0	34.0	30.0	34.0
3	32.08441378883597	33.0	31.0	34.0	30.0	34.0
4	35.822704745109185	37.0	35.0	37.0	35.0	37.0
5	35.765575479122795	37.0	35.0	37.0	35.0	37.0
6	35.80873762189569	37.0	35.0	37.0	35.0	37.0
7	36.11278311801571	37.0	35.0	37.0	35.0	37.0
8	34.77885033733416	37.0	35.0	37.0	35.0	37.0
9	37.137368194097185	39.0	37.0	39.0	34.0	39.0
10	37.1533212283643	39.0	37.0	39.0	32.0	39.0
11	37.05079639480763	39.0	37.0	39.0	33.0	39.0
12	36.356195481713954	38.0	35.0	39.0	33.0	39.0
13	36.02169612660328	38.0	35.0	39.0	32.0	39.0
14	37.081194176465694	39.0	35.0	41.0	32.0	41.0
15	37.36526453998217	39.0	35.0	41.0	33.0	41.0
16	37.47881533734383	39.0	35.0	41.0	33.0	41.0
17	37.390938289796054	39.0	35.0	41.0	33.0	41.0
18	37.332010681765006	39.0	35.0	41.0	33.0	41.0
19	37.23813142593055	38.0	35.0	41.0	33.0	41.0
20	37.084088927047254	38.0	35.0	41.0	33.0	41.0
21	36.96168951334544	38.0	35.0	41.0	32.0	41.0
22	36.872576830779884	38.0	35.0	41.0	32.0	41.0
23	36.81832877946719	38.0	35.0	41.0	32.0	41.0
24	36.67337660956447	38.0	35.0	41.0	32.0	41.0
25	36.58307856821034	38.0	35.0	40.0	32.0	41.0
26	36.47322887479865	38.0	35.0	40.0	32.0	41.0
27	36.34700653397609	37.0	35.0	40.0	31.0	41.0
28	36.38248608218616	37.0	35.0	40.0	31.0	41.0
29	36.39733303941076	37.0	35.0	40.0	31.0	41.0
30	36.34475570586859	37.0	35.0	40.0	31.0	41.0
31	36.21231458223354	37.0	35.0	40.0	31.0	41.0
32	36.04093838648111	37.0	35.0	40.0	31.0	41.0
33	35.86985417959829	37.0	35.0	40.0	30.0	41.0
34	35.69574179675641	37.0	35.0	40.0	30.0	41.0
35	35.53745095650526	37.0	35.0	40.0	30.0	41.0
36	35.34905045606341	37.0	35.0	40.0	29.0	41.0
37	35.25964385092711	37.0	35.0	40.0	28.0	41.0
38	35.1643317225603	37.0	34.0	40.0	28.0	41.0
39	35.126912672123574	37.0	35.0	40.0	27.0	41.0
40	34.928596152321504	36.0	34.0	40.0	27.0	41.0
41	34.85679782961386	36.0	34.0	40.0	26.0	41.0
42	34.769518295713176	36.0	34.0	40.0	26.0	41.0
43	34.65103269308489	36.0	34.0	40.0	26.0	41.0
44	34.51424847672694	35.0	34.0	40.0	25.0	41.0
45	34.38976646691534	35.0	34.0	40.0	24.0	41.0
46	34.324943004159394	35.0	34.0	40.0	24.0	41.0
47	34.263296612349	35.0	34.0	40.0	24.0	41.0
48	34.236762365535256	35.0	34.0	40.0	24.0	41.0
49	34.20123254109598	35.0	34.0	40.0	24.0	41.0
50	34.0796336796592	35.0	34.0	40.0	24.0	41.0
51	33.70732079908265	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	7.0
15	10.0
16	31.0
17	98.0
18	199.0
19	373.0
20	678.0
21	1109.0
22	1701.0
23	2672.0
24	3962.0
25	5858.0
26	7646.0
27	8879.0
28	9362.0
29	10407.0
30	11638.0
31	14185.0
32	17890.0
33	24021.0
34	45010.0
35	82989.0
36	37074.0
37	46195.0
38	68423.0
39	116602.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.55601255358769	23.269965947523218	27.669136003001103	14.504885495887985
2	30.78239481149315	25.969025975407188	29.963859126005765	13.284720087093898
3	29.30949466588545	25.569523323336096	31.97297459310094	13.14800741767751
4	25.31524162562386	28.69921859137608	30.99394171438074	14.99159806861932
5	23.408612318062897	32.73697990691163	29.955544211175635	13.898863563849845
6	21.442038275680034	40.70208820384304	27.31952283991082	10.53635068056611
7	85.6378216470106	4.908700301464005	7.855467443241038	1.5980106082843626
8	83.47052942803055	6.984335087200252	7.034417946293385	2.5107175384758182
9	81.50550234654631	6.003175137244438	9.565826086788373	2.925496429420876
10	49.75761056419598	24.70612577178846	14.302813728504496	11.233449935511068
11	44.33783305584722	22.17645796230443	20.734303664557	12.751405317291347
12	39.097696381851826	21.8258779486525	23.743142612391544	15.333283057104127
13	22.15112647758937	39.272502963396974	24.898142293330856	13.6782282656828
14	17.28052008825412	40.89043069325119	27.052091974560227	14.776957243934463
15	15.845713854775179	24.046733688747597	45.20896541188801	14.898587044589215
16	17.09469141030624	19.920408861765505	44.17791597295139	18.806983754976862
17	17.58217746348689	20.382950170455754	28.77946718799249	33.25540517806487
18	22.29364025037562	23.33223112369306	33.661482413955135	20.712646211976185
19	28.117561293491356	25.314854885399203	25.49236864851695	21.075215172592493
20	30.46391423648778	23.671208930605268	25.21256209597732	20.65231473692963
21	22.627783804479613	28.013721543170846	28.146180070116	21.212314582233542
22	23.368391334698526	24.622009772925477	25.517893503344336	26.49170538903166
23	20.93057432857063	29.878002796131824	24.29946842556121	24.89195444973634
24	21.360242718164997	24.940103607706185	36.311619803419944	17.388033870708874
25	18.759801447568663	25.706816103089476	33.96468675008653	21.568695699255333
26	18.63643131590295	33.4961509679141	26.798970497521964	21.068447218660992
27	19.786016633697063	31.531123886429867	28.84927379854315	19.83358568132992
28	16.93438758718575	29.414301266767605	36.53844294518151	17.11286820086514
29	17.714249250207388	24.89446826119661	35.48244876175449	21.90883372684151
30	19.442591314201294	29.808389555693495	31.770516085492794	18.97850304461242
31	28.399881657491257	27.056732857256115	24.902976546139076	19.640408939113552
32	28.071345836644795	27.49722997314089	26.748114157979515	17.683310032234797
33	26.899522955932888	27.52430178886691	25.109689196218454	20.46648605898175
34	19.673088488097104	29.20758861668823	28.567726914992562	22.551595980222107
35	21.69670671361693	26.6779208072042	29.987256909597537	21.638115569581334
36	30.162063491142682	24.73165062661585	26.586456744072724	18.51982913816875
37	22.185933097808537	30.715102012402756	28.41535126647755	18.683613623311153
38	22.199469005671546	31.107449970317685	24.433280543292668	22.259800480718102
39	22.73336388581108	29.814190659063357	26.747920787867187	20.70452466725838
40	24.791788731550074	26.128749688190695	26.36446785511938	22.714993725139855
41	18.57281254894681	24.443335789133762	28.01874916609139	28.965102495828038
42	23.411319499635496	26.556677746774106	25.06579418071984	24.96620857287056
43	24.392092709366654	25.93035195294145	26.053722084607163	23.623833253084737
44	21.279994121548583	30.28833417449332	27.14742343993828	21.284248264019816
45	19.245933909963007	33.86084699976602	23.932645322473668	22.960573767797303
46	23.67101556049294	29.850544240181147	26.576788238456288	19.901651960869625
47	23.264164844153356	27.099274281968434	27.384688567765593	22.25187230611262
48	25.022092535333552	23.413253200758785	29.850544240181147	21.714110023726512
49	22.91184449949047	23.662313905438147	30.490019201652153	22.93582239341923
50	20.98394447957335	30.467588268622027	27.54943990346964	20.999027348334987
51	20.347370069787274	31.661261972027077	24.89891577378017	23.092452184405474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	998.0
1	1124.0
2	1250.0
3	3525.5
4	5801.0
5	4171.0
6	2541.0
7	2680.0
8	2819.0
9	3065.5
10	3312.0
11	3537.0
12	3762.0
13	3660.0
14	3558.0
15	3445.5
16	3333.0
17	3152.5
18	2972.0
19	2992.0
20	3012.0
21	2944.5
22	2877.0
23	2940.0
24	3003.0
25	3063.5
26	3295.0
27	3466.0
28	4357.5
29	5249.0
30	6184.0
31	7119.0
32	8000.5
33	8882.0
34	9467.0
35	10052.0
36	11522.0
37	12992.0
38	15132.5
39	17273.0
40	23713.5
41	30154.0
42	37217.5
43	44281.0
44	45403.5
45	46526.0
46	46289.5
47	46053.0
48	43936.0
49	41819.0
50	39734.0
51	37649.0
52	35005.0
53	32361.0
54	28686.5
55	25012.0
56	23122.0
57	21232.0
58	20311.0
59	19390.0
60	17843.5
61	16297.0
62	14787.0
63	13277.0
64	11808.5
65	10340.0
66	9187.0
67	8034.0
68	6706.5
69	5379.0
70	4574.5
71	3770.0
72	3478.5
73	3187.0
74	2762.5
75	1859.5
76	1381.0
77	1043.0
78	705.0
79	504.5
80	304.0
81	236.0
82	168.0
83	116.5
84	65.0
85	42.0
86	19.0
87	10.5
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	517143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.502800255587417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69907732125685	20.857459265443
2	6.201953578637564	3.2873827377811393
3	2.3229621894144383	1.8469500872199864
4	1.2966994047336036	1.3746466126077521
5	0.8277949185287378	1.0969441689178698
6	0.6405425599899426	1.0185702913569643
7	0.5932071539297988	1.1005155497550871
8	0.49297315025416505	1.0452135146043058
9	0.41040465586497715	0.9789185356517325
>10	7.618404019691395	50.38626536298844
>50	0.8441353636018926	13.757588648160734
>100	0.04965502138834662	2.2395174408692893
>500	7.302209027698032E-4	0.12753560262122896
>1k	0.0014604418055396064	0.8824921820224645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3426	0.6624860048381203	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1134	0.21928170738074382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	659	0.12743090402461216	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0881767712218864	0.0
2	0.0	0.0	0.0	0.32486178871221305	0.0
3	0.0	0.0	0.0	0.44900540082723733	0.0
4	0.0	0.0	0.0	0.749115815161377	0.0
5	0.0	0.0	0.0	1.1012427897119366	0.0
6	0.0	0.0	0.0	1.615413918393945	0.0
7	0.0	0.0	0.0	1.7998890055555232	0.0
8	0.0	0.0	0.0	2.3163805755854763	0.0
9	0.0	0.0	0.0	2.5557727746484047	0.0
10	0.0	0.0	0.0	2.9309107925660793	0.0
11	0.0	0.0	0.0	3.3101095828426566	0.0
12	0.0	0.0	0.0	3.587982434258996	0.0
13	0.0	0.0	0.0	3.7198608508671684	0.0
14	0.0	0.0	0.0	3.7799989558013936	0.0
15	0.0	0.0	0.0	3.854446449047942	0.0
16	0.0	0.0	0.0	4.005468506776656	0.0
17	0.0	0.0	0.0	4.167899401132762	0.0
18	0.0	0.0	0.0	4.378672823571043	0.0
19	0.0	0.0	0.0	4.479612022206624	0.0
20	0.0	0.0	0.0	4.59447386892987	0.0
21	0.0	0.0	0.0	4.734473830255848	0.0
22	0.0	0.0	0.0	4.870993129559909	0.0
23	0.0	0.0	0.0	5.028203030883141	0.0
24	0.0	0.0	0.0	5.156987525694054	0.0
25	0.0	0.0	0.0	5.259086945003606	0.0
26	0.0	0.0	0.0	5.357125591954256	0.0
27	0.0	0.0	0.0	5.456711199803536	0.0
28	0.0	0.0	0.0	5.5574570283267875	0.0
29	0.0	0.0	0.0	5.660910038422641	0.0
30	0.0	0.0	0.0	5.799169668737661	0.0
31	0.0	0.0	0.0	5.91809228781981	0.0
32	0.0	0.0	0.0	6.0314071736444275	0.0
33	0.0	0.0	0.0	6.145302169806031	0.0
34	0.0	0.0	0.0	6.245467887992296	0.0
35	0.0	0.0	0.0	6.391849063025121	0.0
36	0.0	0.0	0.0	6.505744059186724	0.0
37	0.0	0.0	0.0	6.623893197819559	0.0
38	0.0	0.0	0.0	6.733147311285273	0.0
39	0.0	0.0	0.0	6.854390371715367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	35	1.2101918E-7	45.000004	5
TATAGCG	35	1.2101918E-7	45.000004	1
GCGACGT	20	7.0296845E-4	45.0	17
CATATCC	25	3.8878185E-5	45.0	29
AGTTTAG	50	2.1827873E-11	45.0	1
CGAGTCT	20	7.0296845E-4	45.0	17
ACCATCG	20	7.0296845E-4	45.0	17
CGTGAAC	20	7.0296845E-4	45.0	34
CGACAGG	25	3.8878185E-5	45.0	2
TAACGCC	25	3.8878185E-5	45.0	12
TCTAGCG	20	7.0296845E-4	45.0	1
CTAACGC	20	7.0296845E-4	45.0	33
CTAAACG	20	7.0296845E-4	45.0	23
TGATTCG	20	7.0296845E-4	45.0	15
ATTGGGC	55	1.8189894E-12	45.0	4
TTCGGTA	20	7.0296845E-4	45.0	35
ATAACTC	20	7.0296845E-4	45.0	11
CGCACGG	25	3.8878185E-5	45.0	2
CCACGTT	20	7.0296845E-4	45.0	16
TCGACAC	20	7.0296845E-4	45.0	38
>>END_MODULE