FastQCFastQC Report
Thu 26 May 2016
SRR1547134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547134_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences963694
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68940.715372306977111No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA207.032718E-445.044
TCGACTT207.032718E-445.035
CGTTTTT44350.041.7023661
AAGTACG451.927765E-840.01
ACGGGAT3050.039.8360675
CGAATAT1200.039.37500414
TCGTTAG356.2472373E-638.571431
CGACAGG1050.038.571432
ATGTACG700.038.571431
ACGTAAG356.2472373E-638.571431
GTTGATC5150.038.446616
ATAGGGT2000.038.254
GCCCTAC6250.038.1626
AGGGATT8450.037.8106546
GTATTCG301.13978494E-437.5000041
CGACTGG601.546141E-1037.5000042
TCACGAC900.037.525
CGGTCTA900.037.531
AGGGCGA11350.037.466966
GCCAGTA5350.037.4299125
Produced by FastQC (version 0.11.5)