##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547133_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	794340
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426744215323414	31.0	31.0	33.0	30.0	34.0
2	31.800786816728355	31.0	31.0	34.0	30.0	34.0
3	31.895514515195003	31.0	31.0	34.0	30.0	34.0
4	35.66857768713649	37.0	35.0	37.0	33.0	37.0
5	35.603610544603065	37.0	35.0	37.0	33.0	37.0
6	35.64279401767505	37.0	35.0	37.0	33.0	37.0
7	35.99445829241886	37.0	35.0	37.0	35.0	37.0
8	34.66384923332578	37.0	35.0	37.0	33.0	37.0
9	36.98013696905607	39.0	37.0	39.0	33.0	39.0
10	36.87705138857416	39.0	37.0	39.0	32.0	39.0
11	36.783784021955334	39.0	37.0	39.0	32.0	39.0
12	36.502717979706425	39.0	35.0	39.0	32.0	39.0
13	36.43500390261097	39.0	35.0	39.0	32.0	39.0
14	37.48261197975678	40.0	36.0	41.0	32.0	41.0
15	37.63289145705869	40.0	36.0	41.0	32.0	41.0
16	37.68965808092253	40.0	36.0	41.0	33.0	41.0
17	37.58404461565576	40.0	36.0	41.0	32.0	41.0
18	37.50860840446157	39.0	36.0	41.0	32.0	41.0
19	37.44008359140922	39.0	36.0	41.0	32.0	41.0
20	37.285692524611626	39.0	35.0	41.0	32.0	41.0
21	37.17956794319813	39.0	35.0	41.0	32.0	41.0
22	37.09285318629302	39.0	35.0	41.0	32.0	41.0
23	36.99273862577738	39.0	35.0	41.0	31.0	41.0
24	36.838360148047435	39.0	35.0	41.0	31.0	41.0
25	36.71880932598132	38.0	35.0	41.0	31.0	41.0
26	36.59939068912556	38.0	35.0	41.0	31.0	41.0
27	36.47854319309112	38.0	35.0	41.0	31.0	41.0
28	36.4991603091875	38.0	35.0	41.0	31.0	41.0
29	36.441230455472464	38.0	35.0	41.0	30.0	41.0
30	36.3787546894277	38.0	35.0	40.0	30.0	41.0
31	36.26585970743007	38.0	35.0	40.0	30.0	41.0
32	36.16783996777199	38.0	35.0	41.0	30.0	41.0
33	36.00943298839288	38.0	35.0	41.0	30.0	41.0
34	35.82061711609638	38.0	35.0	41.0	29.0	41.0
35	35.671040108769546	38.0	35.0	41.0	28.0	41.0
36	35.514824886068936	38.0	35.0	40.0	27.0	41.0
37	35.469826522647736	38.0	35.0	40.0	27.0	41.0
38	35.349185487322806	38.0	34.0	40.0	27.0	41.0
39	35.25249641211572	38.0	34.0	40.0	26.0	41.0
40	35.081932170103485	38.0	34.0	40.0	25.0	41.0
41	34.958983558677645	37.0	34.0	40.0	25.0	41.0
42	34.87912984364378	37.0	34.0	40.0	25.0	41.0
43	34.78377772742151	37.0	34.0	40.0	24.0	41.0
44	34.65613213485409	37.0	34.0	40.0	24.0	41.0
45	34.53656368809326	37.0	34.0	40.0	24.0	41.0
46	34.45362313367072	36.0	34.0	40.0	23.0	41.0
47	34.36875141627011	36.0	33.0	40.0	23.0	41.0
48	34.28169675453836	36.0	33.0	40.0	23.0	41.0
49	34.18295062567666	36.0	33.0	40.0	24.0	41.0
50	34.09050784298915	35.0	33.0	40.0	24.0	41.0
51	33.73283606516102	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	5.0
14	9.0
15	24.0
16	78.0
17	210.0
18	414.0
19	794.0
20	1345.0
21	2016.0
22	3079.0
23	4540.0
24	6462.0
25	9177.0
26	12280.0
27	14209.0
28	15367.0
29	16869.0
30	19454.0
31	23334.0
32	28434.0
33	36978.0
34	58814.0
35	80050.0
36	66642.0
37	86546.0
38	121310.0
39	185763.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.04600045320644	23.450159881159202	27.333131908250873	15.17070775738349
2	34.22942316892011	24.64473651081401	27.250799405796005	13.875040914469874
3	29.2155751945011	25.28766019588589	31.408590779766847	14.088173829846163
4	25.850139738650956	28.13618853387718	30.39655563108996	15.617116096381903
5	23.362665860966338	31.615051489286706	29.83810458997407	15.184178059772893
6	21.976357730946447	40.441750383966564	27.10161895410026	10.48027293098673
7	86.37308960898355	3.5465921393861572	8.105471208802276	1.9748470428280083
8	83.85376539013521	5.964448472946094	7.760027192386132	2.4217589445325682
9	81.4035551527054	4.904197195155727	10.386484376967042	3.3057632751718407
10	43.68217639801596	26.73338872523101	16.357479165093032	13.226955711659993
11	34.33794093209457	25.46088576680011	23.88851121685928	16.31266208424604
12	29.54314273484906	21.803509832061838	29.00030213762369	19.653045295465418
13	25.271294407936146	25.405745650477122	30.24246544300929	19.080494498577437
14	22.51793942140645	26.526298562328478	31.274013646549335	19.681748369715738
15	21.10859329758038	22.6512576478586	36.35609436765113	19.884054686909888
16	24.779691316061133	21.16725835284639	33.611299947125914	20.441750383966564
17	24.509781705566887	21.770904146838884	29.470126142457893	24.24918800513634
18	25.473348943777225	22.424024976710225	30.476873882720245	21.625752196792305
19	28.169801344512425	24.336556134652668	27.01110355767052	20.482538963164387
20	29.508648689478058	24.10479139914898	26.23725356900068	20.149306342372284
21	27.691039101644133	25.254425057280255	27.805851398645416	19.248684442430193
22	26.891381524284313	22.802326459702392	27.447692423899085	22.85859959211421
23	25.063071228944782	25.569277639298033	27.010599994964373	22.357051136792812
24	24.091950550142258	24.329002694060478	30.64128710627691	20.937759649520356
25	24.640204446458693	24.451997885036633	28.698038623259563	22.209759045245107
26	22.834680363572275	26.152655034368156	27.319409824508394	23.693254777551175
27	22.850794370168945	24.606843417176524	29.018052723015337	23.524309489639197
28	22.152982350127147	25.35740363068711	30.585769317924317	21.903844701261423
29	23.11503890021905	24.23987209507264	30.216406072966233	22.428682931742074
30	24.000050356270613	24.2542236321978	29.72442530906161	22.021300702469976
31	26.293526701412496	24.578769796308887	27.279124808016718	21.848578694261903
32	27.199939572475262	24.636175944809526	26.340609814437144	21.823274668278067
33	26.177203716292773	24.565047712566408	26.36163355741874	22.896115013722085
34	23.158597074300676	27.084246040738226	27.998715915099325	21.758440969861773
35	24.365133318226455	26.030289296774683	28.09124556235365	21.513331822645217
36	26.21169776166377	26.87526751768764	25.77813027167208	21.134904448976506
37	24.48296699146461	27.356044011380515	25.9713724601556	22.18961653699927
38	24.75778633834378	27.477906186267848	25.37024447969383	22.39406299569454
39	24.894881285092026	25.755973512601653	25.72047234181836	23.62867286048795
40	26.03721328398419	24.34499080998061	26.709595387365614	22.908200518669588
41	23.376262054032278	23.742352141400406	27.321927638039128	25.559458166528188
42	24.479693833874663	24.688672356925245	26.250723871390086	24.580909937810006
43	23.523050582873832	24.299292494397864	27.770350227862124	24.40730669486618
44	23.62011229448347	25.34556990709268	27.57610091396631	23.458216884457535
45	22.75927184832691	25.896845179645993	26.655210615101844	24.688672356925245
46	23.752045723493715	25.844096986177206	26.778079915401463	23.625777374927612
47	23.475086235113427	25.613717048115415	27.942820454717122	22.968376262054033
48	24.001435153712517	24.938439459173654	28.114913009542512	22.945212377571316
49	23.789687035778133	24.606843417176524	28.20605785935494	23.397411687690408
50	22.72314122416094	26.12709922703125	28.300601757433846	22.84915779137397
51	22.696074728705593	26.328272528136566	27.281264949517837	23.694387793640004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	752.0
1	1080.0
2	1408.0
3	5881.0
4	10354.0
5	7106.5
6	3859.0
7	3897.0
8	3935.0
9	4075.5
10	4216.0
11	4309.5
12	4403.0
13	4406.5
14	4410.0
15	4304.0
16	4198.0
17	4127.5
18	4057.0
19	3903.5
20	3750.0
21	3766.0
22	3782.0
23	3939.0
24	4096.0
25	4368.5
26	5078.5
27	5516.0
28	6636.5
29	7757.0
30	8996.0
31	10235.0
32	11798.0
33	13361.0
34	15390.5
35	17420.0
36	18952.5
37	20485.0
38	22703.5
39	24922.0
40	29331.5
41	33741.0
42	36744.5
43	39748.0
44	42204.0
45	44660.0
46	48327.5
47	51995.0
48	54025.0
49	56055.0
50	54757.5
51	53460.0
52	52166.0
53	50872.0
54	48543.0
55	46214.0
56	45549.0
57	44884.0
58	43663.0
59	42442.0
60	41100.0
61	39758.0
62	37740.5
63	35723.0
64	32653.5
65	29584.0
66	25881.5
67	22179.0
68	19142.0
69	16105.0
70	13535.0
71	10965.0
72	9177.5
73	7390.0
74	6163.5
75	3901.5
76	2866.0
77	2300.0
78	1734.0
79	1260.0
80	786.0
81	599.5
82	413.0
83	284.0
84	155.0
85	102.0
86	49.0
87	45.5
88	42.0
89	25.5
90	9.0
91	7.0
92	5.0
93	7.0
94	9.0
95	5.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	794340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.186972555923525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96612635962131	22.84184761537332
2	6.806448403822916	4.381579359543155
3	3.5333637160089233	3.411848428718256
4	2.5453448233623774	3.2770777589970748
5	1.9849801237465394	3.1945250383541777
6	1.5963845368846576	3.0829671166444297
7	1.3687242599557887	3.0838563134276598
8	1.2123615410200197	3.1217798118934823
9	1.0941094523324817	3.16944638238619
>10	8.832589597593298	47.42324694786825
>50	0.03702825108125955	0.7951560783316914
>100	0.02173397262148785	1.3265555537522191
>500	4.024809744719958E-4	0.09910302720902789
>1k	0.0	0.0
>5k	4.024809744719958E-4	0.7910105675010773
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6106	0.7686884709318428	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027947730191102048	0.0
2	0.0	0.0	0.0	0.0936626633431528	0.0
3	0.0	0.0	0.0	0.1642873328801269	0.0
4	0.0	0.0	0.0	0.30931339225016996	0.0
5	0.0	0.0	0.0	0.5187954780068988	0.0
6	0.0	0.0	0.0	1.044011380517159	0.0
7	0.0	0.0	0.0	1.3064934410957525	0.0
8	0.0	0.0	0.0	1.8906261802250925	0.0
9	0.0	0.0	0.0	2.244253090616109	0.0
10	0.0	0.0	0.0	2.704131732003928	0.0
11	0.0	0.0	0.0	3.045799028123977	0.0
12	0.0	0.0	0.0	3.3085328700556436	0.0
13	0.0	0.0	0.0	3.470302389405041	0.0
14	0.0	0.0	0.0	3.5574187375682955	0.0
15	0.0	0.0	0.0	3.6348415036382407	0.0
16	0.0	0.0	0.0	3.742100360047335	0.0
17	0.0	0.0	0.0	3.8425611199234586	0.0
18	0.0	0.0	0.0	3.9948888385326184	0.0
19	0.0	0.0	0.0	4.085656016315432	0.0
20	0.0	0.0	0.0	4.182591837248533	0.0
21	0.0	0.0	0.0	4.293375632600649	0.0
22	0.0	0.0	0.0	4.418133293048317	0.0
23	0.0	0.0	0.0	4.546919455145152	0.0
24	0.0	0.0	0.0	4.654178311554246	0.0
25	0.0	0.0	0.0	4.757786338343783	0.0
26	0.0	0.0	0.0	4.857491754160687	0.0
27	0.0	0.0	0.0	4.9657577359820735	0.0
28	0.0	0.0	0.0	5.061560540826346	0.0
29	0.0	0.0	0.0	5.18140846488909	0.0
30	0.0	0.0	0.0	5.341541405443513	0.0
31	0.0	0.0	0.0	5.469572223481129	0.0
32	0.0	0.0	0.0	5.604401138051716	0.0
33	0.0	0.0	0.0	5.730291814588211	0.0
34	0.0	0.0	0.0	5.8692751214845025	0.0
35	0.0	0.0	0.0	6.035828486542287	0.0
36	0.0	0.0	0.0	6.16612533675756	0.0
37	0.0	0.0	0.0	6.301080142004683	0.0
38	0.0	0.0	0.0	6.477704761185387	0.0
39	0.0	0.0	0.0	6.770274693456202	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	3.889713E-5	45.000004	1
CGCATCG	20	7.0319686E-4	45.0	21
CGTTAGT	20	7.0319686E-4	45.0	28
GCGATAT	85	0.0	42.35294	9
TACGTAG	60	3.6379788E-12	41.249996	1
CGTTTTT	4020	0.0	40.80224	1
CGTTAGG	105	0.0	40.714287	2
CTACGAA	40	3.4564255E-7	39.375	11
ACACGAC	375	0.0	39.0	26
GGTACCT	470	0.0	38.776596	8
GGGCGAT	1890	0.0	38.57143	7
TTAACGG	70	0.0	38.571426	2
TAGGGAC	475	0.0	38.36842	5
CACGACC	390	0.0	38.076923	27
GCGATTC	65	9.094947E-12	38.07692	9
GTTGATC	470	0.0	37.81915	16
AACGGGA	245	0.0	37.65306	4
TAGACGG	60	1.546141E-10	37.499996	2
TAGTTCG	30	1.1396045E-4	37.499996	1
CGAATAT	30	1.1396045E-4	37.499996	14
>>END_MODULE