##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547132_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	909773
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.376746726930783	31.0	31.0	33.0	30.0	34.0
2	31.73951414253885	31.0	31.0	34.0	30.0	34.0
3	31.869321248267426	31.0	31.0	34.0	30.0	34.0
4	35.6293976629335	37.0	35.0	37.0	33.0	37.0
5	35.55058349720205	37.0	35.0	37.0	33.0	37.0
6	35.59246757158104	37.0	35.0	37.0	33.0	37.0
7	35.959223894312096	37.0	35.0	37.0	35.0	37.0
8	34.63759421306194	37.0	35.0	37.0	33.0	37.0
9	36.94028070738525	39.0	37.0	39.0	33.0	39.0
10	36.828171422981335	39.0	37.0	39.0	32.0	39.0
11	36.726131683397945	39.0	35.0	39.0	32.0	39.0
12	36.32417756956955	38.0	35.0	39.0	32.0	39.0
13	36.175254706393794	39.0	35.0	39.0	32.0	39.0
14	37.16381668833874	39.0	35.0	41.0	32.0	41.0
15	37.37135197461345	39.0	35.0	41.0	32.0	41.0
16	37.443310584068776	39.0	35.0	41.0	32.0	41.0
17	37.34813519416382	39.0	35.0	41.0	32.0	41.0
18	37.30141255016361	39.0	35.0	41.0	32.0	41.0
19	37.24973812148745	39.0	35.0	41.0	32.0	41.0
20	37.12957408056735	39.0	35.0	41.0	32.0	41.0
21	37.02763436593524	39.0	35.0	41.0	32.0	41.0
22	36.94113146905877	39.0	35.0	41.0	31.0	41.0
23	36.8740949665466	38.0	35.0	41.0	31.0	41.0
24	36.727005527752524	38.0	35.0	41.0	31.0	41.0
25	36.62286856171814	38.0	35.0	41.0	31.0	41.0
26	36.4834568623162	38.0	35.0	40.0	31.0	41.0
27	36.38020693073987	38.0	35.0	40.0	30.0	41.0
28	36.41301401558411	38.0	35.0	40.0	31.0	41.0
29	36.377953621397864	38.0	35.0	40.0	30.0	41.0
30	36.297979825736746	38.0	35.0	40.0	30.0	41.0
31	36.21291794766387	38.0	35.0	40.0	30.0	41.0
32	36.14144627286147	38.0	35.0	40.0	30.0	41.0
33	36.02490181616733	38.0	35.0	41.0	30.0	41.0
34	35.867003087583385	38.0	35.0	41.0	29.0	41.0
35	35.76502929851732	38.0	35.0	41.0	29.0	41.0
36	35.63973320817391	38.0	35.0	40.0	29.0	41.0
37	35.6063644447571	38.0	35.0	40.0	29.0	41.0
38	35.49106425448986	38.0	34.0	40.0	28.0	41.0
39	35.39832133949897	38.0	34.0	40.0	27.0	41.0
40	35.24571294157993	37.0	34.0	40.0	27.0	41.0
41	35.12868814528459	37.0	34.0	40.0	27.0	41.0
42	35.060060036954276	37.0	34.0	40.0	27.0	41.0
43	34.96261265172741	37.0	34.0	40.0	26.0	41.0
44	34.83704946178882	37.0	34.0	40.0	26.0	41.0
45	34.70482526960022	36.0	34.0	40.0	26.0	41.0
46	34.64053340778414	36.0	34.0	40.0	26.0	41.0
47	34.54802022042861	36.0	34.0	40.0	26.0	41.0
48	34.470041427916634	36.0	34.0	40.0	25.0	41.0
49	34.38939713532936	36.0	34.0	40.0	25.0	41.0
50	34.27843538992694	35.0	33.0	40.0	25.0	41.0
51	33.91206158019638	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	5.0
14	19.0
15	33.0
16	94.0
17	198.0
18	409.0
19	886.0
20	1436.0
21	2304.0
22	3411.0
23	4848.0
24	6753.0
25	9587.0
26	12505.0
27	14904.0
28	16646.0
29	19051.0
30	23096.0
31	27827.0
32	34336.0
33	43785.0
34	72390.0
35	104982.0
36	73737.0
37	96049.0
38	134222.0
39	206125.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.009062700256	23.254152409447194	28.09810798957542	13.63867690072139
2	31.367604886053996	25.680581859430866	29.11704348227525	13.834769772239888
3	29.18563202029517	26.084748613115575	30.47936133519021	14.25025803139904
4	25.804568832005348	28.619226993986413	30.250073369950524	15.326130804057717
5	23.23722510999997	32.05711754470621	29.649813744747316	15.05584360054651
6	22.09782000564976	41.189395596483955	25.903934278111134	10.808850119755148
7	87.47390832658256	4.104430445836489	6.600657526657748	1.8210037009231974
8	84.89612243933377	6.098774089800423	6.113393121141208	2.8917103497246015
9	81.9335152834828	5.335506769271016	9.024998543592742	3.705979403653439
10	45.47431062473826	25.839192853601944	14.6882793839782	13.998217137681598
11	37.89022096720831	23.661396853940488	21.677055705104458	16.771326473746747
12	33.28830378566961	20.621737510345987	26.706991744094406	19.382966959889995
13	25.287956446278358	27.891902705400142	28.17351141438579	18.64662943393572
14	21.208147526910558	29.420086109392123	29.888774452528267	19.48299191116905
15	19.95244967700734	22.71522676535795	37.66510986806599	19.667213689568715
16	23.492013941939362	20.277365892370955	35.686924100847136	20.54369606484255
17	22.984524711109255	21.526688525599244	28.112287350800695	27.37649941249081
18	25.57440152653464	22.10254645939152	30.49804731509948	21.82500469897436
19	28.413351462397763	25.34577306646823	24.750679565122287	21.49019590601172
20	30.43660341645663	24.52633788868212	24.006208142031035	21.03085055283021
21	26.78822079793531	26.386692064943674	26.073537025170012	20.751550111951005
22	25.978678197748227	23.26745243044144	25.895250793329765	24.858618578480566
23	24.303644975175125	26.469130211602238	25.59297758891504	23.634247224307604
24	24.48116178431323	23.600282707884272	30.930023203590345	20.98853230421215
25	24.461046876528542	23.75185898020715	28.0340260702395	23.75306807302481
26	21.932943712332637	27.064883218121444	26.717214074280065	24.28495899526585
27	22.64696797992466	25.543294865862144	27.465532610882054	24.344204543331138
28	21.2916848488579	26.309859712257893	29.65234184791151	22.746113590972694
29	22.714347425126928	24.466432835443566	29.20299898985791	23.616220749571596
30	24.314636728062936	25.076035450601413	28.539866538136437	22.069461283199214
31	27.829579466526265	24.899288064165457	24.51545605332319	22.75567641598509
32	29.853490925758404	23.6101752854833	24.70693238862881	21.829401400129484
33	27.641070904500353	24.0816115668414	23.849685580908645	24.427631947749603
34	23.16874648950892	24.669340593752505	27.85442082805271	24.30749208868586
35	22.942646132606708	25.015580809718468	28.22770075612268	23.814072301552144
36	26.83735393334381	26.27127865962169	24.480392361611084	22.410975045423417
37	23.302735957211304	27.860356374612127	26.1398172950835	22.69709037309307
38	23.751529227620516	28.335529851952078	24.955126168835523	22.957814751591883
39	23.22656310969879	26.6774239288262	25.197933990127208	24.898078971347797
40	25.70113643733107	24.365088873817975	26.274356350430274	23.659418338420682
41	21.848966720269782	23.813632631436633	27.36078120586124	26.97661944243234
42	23.54730245896504	25.33599040639808	25.480971626988268	25.63573550764861
43	23.15225886017721	24.08688760822755	27.311648070452737	25.449205461142505
44	23.370884825115716	25.29872836410841	27.03993193906612	24.29045487170976
45	22.033408333727206	26.757443889849448	25.254321682441667	25.954826093981687
46	24.013132946350353	26.149160285038135	26.207306657814645	23.63040011079687
47	22.717315198406634	25.56483870152225	27.75780332016888	23.96004277990224
48	23.41012538292519	23.89980797407705	28.588889756016062	24.101176886981698
49	23.252723481571778	23.35296826790859	28.671327902674626	24.72298034784501
50	22.263245886611273	26.072437849881236	28.09667906170001	23.567637201807486
51	21.19385824815641	26.92221026563769	26.66302473254317	25.220906753662724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	846.0
1	1207.5
2	1569.0
3	4841.0
4	8113.0
5	5620.5
6	3128.0
7	3183.0
8	3238.0
9	3560.0
10	3882.0
11	4018.0
12	4154.0
13	4186.0
14	4218.0
15	3987.5
16	3757.0
17	3834.0
18	3911.0
19	3696.0
20	3481.0
21	3602.0
22	3723.0
23	3624.0
24	3525.0
25	3828.0
26	4972.5
27	5814.0
28	5969.5
29	6125.0
30	7524.0
31	8923.0
32	10611.5
33	12300.0
34	15136.0
35	17972.0
36	19016.5
37	20061.0
38	23353.0
39	26645.0
40	32729.5
41	38814.0
42	44259.0
43	49704.0
44	53428.5
45	57153.0
46	61291.5
47	65430.0
48	69095.0
49	72760.0
50	69560.5
51	66361.0
52	64188.5
53	62016.0
54	59125.0
55	56234.0
56	55120.0
57	54006.0
58	52969.0
59	51932.0
60	49196.5
61	46461.0
62	43665.0
63	40869.0
64	35955.0
65	31041.0
66	26820.0
67	22599.0
68	18855.0
69	15111.0
70	13187.0
71	11263.0
72	9485.0
73	7707.0
74	6269.0
75	3926.0
76	3021.0
77	2247.0
78	1473.0
79	1145.5
80	818.0
81	588.5
82	359.0
83	271.5
84	184.0
85	123.5
86	63.0
87	44.0
88	25.0
89	19.0
90	13.0
91	10.5
92	8.0
93	4.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	909773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.5919066725123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21113061904991	21.504296273940074
2	6.469275613954034	3.6993784918586696
3	2.8206810461149936	2.419459476703328
4	1.7642176601749766	2.0176938675888385
5	1.3394139986339753	1.9148200022399569
6	1.0891680518621745	1.86848347737152
7	0.8579890792823017	1.7172080576612028
8	0.7404706558664068	1.6937174309013026
9	0.6502297631609224	1.6732177833588184
>10	8.759215003637548	54.39229175156524
>50	0.26648419958295066	4.607176849870935
>100	0.028551877811830245	1.490410453486145
>500	0.0027758770094834942	0.4766592275736593
>1k	3.96553858497642E-4	0.5251868558803973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4632	0.5091379937632794	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1983505775616556E-4	0.0	0.0	0.05363975409250439	0.0
2	2.1983505775616556E-4	0.0	0.0	0.1831226031108859	0.0
3	2.1983505775616556E-4	0.0	0.0	0.28380705956320973	0.0
4	2.1983505775616556E-4	0.0	0.0	0.5355182006940192	0.0
5	2.1983505775616556E-4	0.0	0.0	0.8946187675387157	0.0
6	2.1983505775616556E-4	0.0	0.0	1.5852306014797097	0.0
7	2.1983505775616556E-4	0.0	0.0	1.910806322016591	0.0
8	2.1983505775616556E-4	0.0	0.0	2.7978407800627187	0.0
9	2.1983505775616556E-4	0.0	0.0	3.215967059914946	0.0
10	2.1983505775616556E-4	0.0	0.0	3.817655612993571	0.0
11	2.1983505775616556E-4	0.0	0.0	4.311734905300553	0.0
12	2.1983505775616556E-4	0.0	0.0	4.706998339146138	0.0
13	2.1983505775616556E-4	0.0	0.0	4.915072221312349	0.0
14	2.1983505775616556E-4	0.0	0.0	5.0192740386887715	0.0
15	2.1983505775616556E-4	0.0	0.0	5.1092964948399215	0.0
16	2.1983505775616556E-4	0.0	0.0	5.282966190467293	0.0
17	2.1983505775616556E-4	0.0	0.0	5.453887947872711	0.0
18	2.1983505775616556E-4	0.0	0.0	5.691639562836004	0.0
19	2.1983505775616556E-4	0.0	0.0	5.8145273601217005	0.0
20	2.1983505775616556E-4	0.0	0.0	5.9522540238059385	0.0
21	2.1983505775616556E-4	0.0	0.0	6.125703884375553	0.0
22	2.1983505775616556E-4	0.0	0.0	6.317509972267808	0.0
23	2.1983505775616556E-4	0.0	0.0	6.5250342667896275	0.0
24	2.1983505775616556E-4	0.0	0.0	6.687932044586947	0.0
25	2.1983505775616556E-4	0.0	0.0	6.830165326955186	0.0
26	2.1983505775616556E-4	0.0	0.0	6.965473805004105	0.0
27	2.1983505775616556E-4	0.0	0.0	7.109905437949906	0.0
28	2.1983505775616556E-4	0.0	0.0	7.249500699625071	0.0
29	2.1983505775616556E-4	0.0	0.0	7.413607570240049	0.0
30	2.1983505775616556E-4	0.0	0.0	7.644654215941779	0.0
31	2.1983505775616556E-4	0.0	0.0	7.816235478520466	0.0
32	2.1983505775616556E-4	0.0	0.0	7.99946799916023	0.0
33	2.1983505775616556E-4	0.0	0.0	8.172917859729845	0.0
34	2.1983505775616556E-4	0.0	0.0	8.356480132956243	0.0
35	2.1983505775616556E-4	0.0	0.0	8.58532842808041	0.0
36	2.1983505775616556E-4	0.0	0.0	8.762075814516368	0.0
37	2.1983505775616556E-4	0.0	0.0	8.946297592916036	0.0
38	2.1983505775616556E-4	0.0	0.0	9.168111166192006	0.0
39	2.1983505775616556E-4	0.0	0.0	9.556339878189394	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	7.032511E-4	45.0	45
CCCCGAT	40	6.8121153E-9	45.0	40
GTATGCG	35	1.2115561E-7	45.0	1
TTACGTA	20	7.032511E-4	45.0	36
ATACGTC	20	7.032511E-4	45.0	42
CGTTTTT	2815	0.0	41.4032	1
CGTAAGG	120	0.0	41.249996	2
CATAGCG	55	6.002665E-11	40.909092	1
TCGATAC	50	1.0804797E-9	40.5	25
GTGTACG	50	1.0804797E-9	40.5	1
CTCGATA	50	1.0804797E-9	40.5	24
TAACGGG	195	0.0	40.384617	3
CGGTCTA	90	0.0	40.0	31
CATACGA	175	0.0	39.857143	18
AGGGCGA	1025	0.0	39.512196	6
TTGTACG	40	3.4571713E-7	39.375	1
CGTACAT	40	3.4571713E-7	39.375	35
CCGGTAT	40	3.4571713E-7	39.375	42
TGTACGG	160	0.0	39.375	2
TACGATC	35	6.2468553E-6	38.571426	11
>>END_MODULE