FastQCFastQC Report
Thu 26 May 2016
SRR1547131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547131_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1198730
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44140.36822303604648254No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC253.8909056E-545.010
ATATACG253.8909056E-545.01
CGGTCTA850.045.031
CATACGA2200.042.95454418
CGTTAGG1350.041.6666642
ATACGAA2300.041.08695619
GCGTAAG1100.040.9090921
CGTTTTT36550.040.7523961
CTTAACG403.45839E-739.3751
ATTGGGC5000.038.74
AGGGCGA13750.038.6181836
GTTAGCG1050.038.571431
GTAGGGT7900.038.4493684
GCGTTAG1000.038.251
GGGCGAT26950.038.153997
AGGGTAC7850.037.8343936
GCGGGTC2500.037.8000035
TAGGGTC6400.037.6171885
ACGGGAT2600.037.2115365
TTACGGG2300.037.1739123
Produced by FastQC (version 0.11.5)