##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547131_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1198730
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.427570845811818	31.0	31.0	33.0	30.0	34.0
2	31.80184695469372	31.0	31.0	34.0	30.0	34.0
3	31.93566691415081	33.0	31.0	34.0	30.0	34.0
4	35.682052672411636	37.0	35.0	37.0	33.0	37.0
5	35.622539687836294	37.0	35.0	37.0	33.0	37.0
6	35.6678192753998	37.0	35.0	37.0	33.0	37.0
7	36.021156557356534	37.0	35.0	37.0	35.0	37.0
8	34.722226022540525	37.0	35.0	37.0	33.0	37.0
9	37.046853753555844	39.0	37.0	39.0	34.0	39.0
10	36.81314891593603	39.0	37.0	39.0	32.0	39.0
11	36.81185421237476	39.0	37.0	39.0	32.0	39.0
12	36.577932478539786	39.0	35.0	39.0	32.0	39.0
13	36.50413604397988	39.0	35.0	39.0	32.0	39.0
14	37.601316393182785	40.0	36.0	41.0	33.0	41.0
15	37.735275666747306	40.0	36.0	41.0	33.0	41.0
16	37.757834541556484	40.0	36.0	41.0	33.0	41.0
17	37.64156815963561	40.0	36.0	41.0	33.0	41.0
18	37.60556839321615	39.0	36.0	41.0	33.0	41.0
19	37.581406154847215	39.0	36.0	41.0	33.0	41.0
20	37.47564505768605	39.0	35.0	41.0	32.0	41.0
21	37.376770415356255	39.0	35.0	41.0	32.0	41.0
22	37.298289022548865	39.0	35.0	41.0	32.0	41.0
23	37.220052055091635	39.0	35.0	41.0	32.0	41.0
24	37.09607000742452	39.0	35.0	41.0	32.0	41.0
25	36.997517372552615	39.0	35.0	41.0	31.0	41.0
26	36.89768922109232	39.0	35.0	41.0	31.0	41.0
27	36.817552743320014	39.0	35.0	41.0	31.0	41.0
28	36.845121086483196	39.0	35.0	41.0	31.0	41.0
29	36.80386742635957	39.0	35.0	41.0	31.0	41.0
30	36.74919289581474	39.0	35.0	41.0	31.0	41.0
31	36.6542057010336	39.0	35.0	41.0	31.0	41.0
32	36.58491069715449	39.0	35.0	41.0	31.0	41.0
33	36.48382371343005	39.0	35.0	41.0	30.0	41.0
34	36.36534332168211	39.0	35.0	41.0	30.0	41.0
35	36.24906359230185	39.0	35.0	41.0	30.0	41.0
36	36.12658063116799	39.0	35.0	41.0	30.0	41.0
37	36.085105903748136	39.0	35.0	41.0	30.0	41.0
38	35.96389261968917	38.0	35.0	41.0	29.0	41.0
39	35.892268484145724	38.0	35.0	41.0	29.0	41.0
40	35.748708216195475	38.0	35.0	41.0	28.0	41.0
41	35.675766019036814	38.0	35.0	40.0	28.0	41.0
42	35.60949922000784	38.0	35.0	40.0	28.0	41.0
43	35.518327730181106	38.0	35.0	40.0	28.0	41.0
44	35.39806461838779	38.0	34.0	40.0	27.0	41.0
45	35.27937316993818	38.0	34.0	40.0	27.0	41.0
46	35.20293226998574	38.0	34.0	40.0	27.0	41.0
47	35.12476621090654	37.0	34.0	40.0	27.0	41.0
48	35.0481542966306	37.0	34.0	40.0	26.0	41.0
49	34.97625570395335	37.0	34.0	40.0	26.0	41.0
50	34.862834833532155	37.0	34.0	40.0	26.0	41.0
51	34.50766978385458	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	3.0
13	5.0
14	13.0
15	29.0
16	72.0
17	200.0
18	472.0
19	864.0
20	1564.0
21	2507.0
22	3808.0
23	5505.0
24	7954.0
25	11473.0
26	15236.0
27	17704.0
28	19888.0
29	22552.0
30	26700.0
31	32691.0
32	40202.0
33	51607.0
34	83238.0
35	124249.0
36	93164.0
37	123330.0
38	186732.0
39	326736.0
40	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.60393082679168	24.143718769030556	27.207461229801538	13.044889174376214
2	31.87014590441551	25.24688628798812	28.684941563154336	14.198026244442035
3	30.28471799320948	25.811483820376562	30.52588990014432	13.377908286269635
4	25.295521093156925	29.069181550474255	29.94360698405813	15.691690372310696
5	22.791871397228732	32.82165291600277	29.6670643097278	14.7194113770407
6	21.197600794173834	41.12076948103409	26.965872214760623	10.71575751003145
7	89.65004629899977	3.2468529193396343	5.741159393691657	1.3619413879689337
8	87.58944883334863	5.406305006131489	5.057185521343422	1.9470606391764618
9	85.2658229960041	4.071308801815254	7.703319346308176	2.9595488558724647
10	37.75061940553753	26.86401441525615	20.058228291608618	15.327137887597708
11	33.36481109173876	26.29157525047342	22.341561485905917	18.002052171881907
12	29.866024876327447	24.64800246927999	25.77853227999633	19.70744037439624
13	23.691990690147072	30.63475511583092	27.313239845503155	18.36001434851885
14	21.92854103926656	31.55756508971995	27.90202964804418	18.61186422296931
15	21.470222652307026	24.661767036780592	34.57492512909496	19.293085181817425
16	22.826741634896933	23.30791754606959	34.11377040701409	19.751570412019387
17	21.99861520108782	23.531320647685465	28.55997597457309	25.910088176653623
18	24.443702918922526	23.67105186322191	30.698572656060996	21.186672561794566
19	26.899468604272858	26.956445571563236	25.321632060597466	20.822453763566443
20	29.584310061481734	25.509163865090557	24.841206944015752	20.06531912941196
21	25.615276167277035	27.47924887172257	26.9615342904574	19.943940670542993
22	25.14736429387769	23.689988571237894	26.94676866350221	24.21587847138221
23	22.912248796643116	28.304288705546703	26.33829135835426	22.445171139455926
24	22.42172966389429	26.435143860585786	31.628139781268512	19.514986694251416
25	22.80880598633554	26.400023358053943	29.706439314941647	21.084731340668874
26	21.92195073119051	29.106888123263786	26.681988437763298	22.289172707782402
27	21.824847964095333	27.456558190751878	27.832873124056295	22.885720721096494
28	19.83165516838654	28.416907894188014	30.554837202706196	21.196599734719246
29	21.074971010986626	26.117974856723364	30.298649403952517	22.508404728337492
30	22.872456683323183	26.40044046616002	30.17326670726519	20.55383614325161
31	26.049902813811283	27.083079592568804	26.24243991557732	20.624577678042595
32	25.763516388177486	26.351889082612434	26.572372427485757	21.312222101724327
33	24.74218547963261	26.542590908711723	25.371351346842076	23.343872264813594
34	21.344673112377265	27.975190409850427	28.229042403210062	22.451094074562246
35	21.51602112235449	27.892853269710443	28.411318645566556	22.179806962368506
36	25.965730398004556	29.114312647551994	24.757535057936316	20.162421896507137
37	22.41088485313624	29.757993876853	25.80330850149742	22.027812768513343
38	23.041635731148798	30.09993910221651	25.765184820601807	21.093240346032886
39	22.96263545585745	28.979419886046067	24.717826366237603	23.340118291858882
40	24.884669608669174	26.415623201221294	26.61049610838137	22.089211081728163
41	21.060038540789	26.666638859459596	26.99048159301928	25.282841006732127
42	23.716099538678435	27.859234356360478	24.94164657595956	23.483019529001524
43	22.65572731140457	26.09828735411644	27.256012613349128	23.989972721129863
44	22.627530803433636	27.13755391122271	27.6458418492905	22.589073436053155
45	21.642738564981272	28.4231645157792	25.879305598425002	24.054791320814527
46	23.178363768321475	28.15087634413087	26.451577919965295	22.219181967582358
47	22.746740300150993	27.940570437045874	27.328589423806864	21.984099838996272
48	23.273380994886256	27.06756317102267	28.021155723140325	21.637900110950756
49	23.129728963152672	26.136828143118134	28.516513309919667	22.21692958380953
50	21.917279120402426	28.21244150058812	28.218114170830795	21.652165208178655
51	21.475561636064835	28.46037055884144	27.03044054958164	23.033627255512084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1015.0
1	1265.5
2	1516.0
3	5055.0
4	8594.0
5	6291.5
6	3989.0
7	4131.0
8	4273.0
9	4605.5
10	4938.0
11	4983.5
12	5029.0
13	5163.5
14	5298.0
15	5121.0
16	4944.0
17	4828.5
18	4713.0
19	4682.0
20	4651.0
21	4786.5
22	4922.0
23	4874.5
24	4827.0
25	5826.0
26	7715.5
27	8606.0
28	10292.5
29	11979.0
30	14094.5
31	16210.0
32	19308.0
33	22406.0
34	25957.5
35	29509.0
36	33139.5
37	36770.0
38	42017.5
39	47265.0
40	54945.5
41	62626.0
42	71495.5
43	80365.0
44	83803.0
45	87241.0
46	91476.5
47	95712.0
48	98646.0
49	101580.0
50	100726.0
51	99872.0
52	95290.5
53	90709.0
54	81987.0
55	73265.0
56	68417.5
57	63570.0
58	57857.0
59	52144.0
60	47104.5
61	42065.0
62	37645.0
63	33225.0
64	29308.5
65	25392.0
66	21007.5
67	16623.0
68	13883.0
69	11143.0
70	9628.0
71	8113.0
72	7451.5
73	6790.0
74	5753.0
75	3871.5
76	3027.0
77	2158.0
78	1289.0
79	902.5
80	516.0
81	386.0
82	256.0
83	168.5
84	81.0
85	67.5
86	54.0
87	59.5
88	65.0
89	36.5
90	8.0
91	4.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1198730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.323395549476185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47975433811524	19.11403675081383
2	6.181989670037234	3.1309793939425727
3	2.6305829679612613	1.9984587907019415
4	1.6566393994619228	1.6780693918168383
5	1.2677020454806107	1.6051260168292776
6	0.9790065248646646	1.4875061684799593
7	0.8428073299039697	1.4939920371009268
8	0.7092805913376612	1.4369114376007974
9	0.6382853139116182	1.4547196329844936
>10	8.80084361846366	51.817108815454674
>50	0.7627988865411259	11.933457514795032
>100	0.04724996375017558	2.005521208458343
>500	0.002379494577341697	0.37359267081711617
>1k	6.798555935261992E-4	0.47052017020422965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4414	0.36822303604648254	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03144995119835159	0.0
2	0.0	0.0	0.0	0.0934322157616811	0.0
3	0.0	0.0	0.0	0.1530786749309686	0.0
4	0.0	0.0	0.0	0.28321640402759585	0.0
5	0.0	0.0	0.0	0.4625728896415373	0.0
6	0.0	0.0	0.0	0.9212249630859326	0.0
7	0.0	0.0	0.0	1.12402292426151	0.0
8	0.0	0.0	0.0	1.6361482569052248	0.0
9	0.0	0.0	0.0	1.8929200070074161	0.0
10	0.0	0.0	0.0	2.2744905024484248	0.0
11	0.0	0.0	0.0	2.59157608468963	0.0
12	0.0	0.0	0.0	2.8580247428528525	0.0
13	0.0	0.0	0.0	3.0156916069506896	0.0
14	0.0	0.0	0.0	3.086266298499245	0.0
15	0.0	0.0	0.0	3.152753330608227	0.0
16	0.0	0.0	0.0	3.274715740825707	0.0
17	0.0	0.0	0.0	3.400682388861545	0.0
18	0.0	0.0	0.0	3.575867793414697	0.0
19	0.0	0.0	0.0	3.6657128794640994	0.0
20	0.0	0.0	0.0	3.7583108790136226	0.0
21	0.0	0.0	0.0	3.8808572405796133	0.0
22	0.0	0.0	0.0	4.024342429070766	0.0
23	0.0	0.0	0.0	4.175001876986477	0.0
24	0.0	0.0	0.0	4.305473292567968	0.0
25	0.0	0.0	0.0	4.420094600118459	0.0
26	0.0	0.0	0.0	4.528292442835334	0.0
27	0.0	0.0	0.0	4.6367405504158565	0.0
28	0.0	0.0	0.0	4.752362917420937	0.0
29	0.0	0.0	0.0	4.87349111142626	0.0
30	0.0	0.0	0.0	5.0384990781910854	0.0
31	0.0	0.0	0.0	5.165466785681513	0.0
32	0.0	0.0	0.0	5.3147914876577715	0.0
33	0.0	0.0	0.0	5.456357978860961	0.0
34	8.342162121578671E-5	0.0	0.0	5.599676324109683	0.0
35	8.342162121578671E-5	0.0	0.0	5.753922901737672	0.0
36	8.342162121578671E-5	0.0	0.0	5.892152528092231	0.0
37	8.342162121578671E-5	0.0	0.0	6.036805619280405	0.0
38	8.342162121578671E-5	0.0	0.0	6.2082370508788465	0.0
39	8.342162121578671E-5	0.0	0.0	6.435143860585787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGC	25	3.8909056E-5	45.0	10
ATATACG	25	3.8909056E-5	45.0	1
CGGTCTA	85	0.0	45.0	31
CATACGA	220	0.0	42.954544	18
CGTTAGG	135	0.0	41.666664	2
ATACGAA	230	0.0	41.086956	19
GCGTAAG	110	0.0	40.909092	1
CGTTTTT	3655	0.0	40.752396	1
CTTAACG	40	3.45839E-7	39.375	1
ATTGGGC	500	0.0	38.7	4
AGGGCGA	1375	0.0	38.618183	6
GTTAGCG	105	0.0	38.57143	1
GTAGGGT	790	0.0	38.449368	4
GCGTTAG	100	0.0	38.25	1
GGGCGAT	2695	0.0	38.15399	7
AGGGTAC	785	0.0	37.834393	6
GCGGGTC	250	0.0	37.800003	5
TAGGGTC	640	0.0	37.617188	5
ACGGGAT	260	0.0	37.211536	5
TTACGGG	230	0.0	37.173912	3
>>END_MODULE