FastQCFastQC Report
Thu 26 May 2016
SRR1547130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547130_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1205928
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93420.7746731148128246No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT207.033425E-445.015
CGTTTTT51650.040.687321
ACGGGAT3000.039.05
AGGGCGA14850.038.6363646
GTATGCG356.2485433E-638.571431
AGTACGG700.038.571432
GCGTTAG1000.038.251
GTTGATC6550.038.12977216
TTGATCC6750.038.00000417
AAGGGAT11500.037.3695645
TAGGGAT12600.036.7857135
GTAGCAT6550.036.75572629
GGGCGAT29550.036.472087
GCCAGTA6550.036.41221225
AGGGATC8850.036.3559346
CATATGC6650.036.20300733
GACCGAT8850.036.1016969
GCTAACG250.002107149536.01
TAAGGGA10450.035.9569364
ATGGGAC6950.035.9352535
Produced by FastQC (version 0.11.5)