##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547130_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1205928
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5262254462953	31.0	31.0	33.0	30.0	34.0
2	31.89458491717582	31.0	31.0	34.0	30.0	34.0
3	31.985705614265527	33.0	31.0	34.0	30.0	34.0
4	35.75304744561864	37.0	35.0	37.0	35.0	37.0
5	35.690214507002075	37.0	35.0	37.0	33.0	37.0
6	35.74300206977531	37.0	35.0	37.0	33.0	37.0
7	36.069307620355445	37.0	35.0	37.0	35.0	37.0
8	34.74827104105718	37.0	35.0	37.0	33.0	37.0
9	37.06292995933422	39.0	37.0	39.0	34.0	39.0
10	37.00438998016465	39.0	37.0	39.0	32.0	39.0
11	36.918000908843645	39.0	37.0	39.0	33.0	39.0
12	36.62413095972562	39.0	35.0	39.0	33.0	39.0
13	36.56974960362476	39.0	35.0	39.0	33.0	39.0
14	37.652274430977634	40.0	36.0	41.0	33.0	41.0
15	37.79493220159081	40.0	36.0	41.0	33.0	41.0
16	37.81450385097618	40.0	36.0	41.0	33.0	41.0
17	37.69949698489462	40.0	36.0	41.0	33.0	41.0
18	37.62146662155618	39.0	36.0	41.0	33.0	41.0
19	37.55823979541067	39.0	36.0	41.0	33.0	41.0
20	37.433331840706906	39.0	35.0	41.0	32.0	41.0
21	37.35289503187587	39.0	35.0	41.0	32.0	41.0
22	37.27601316164812	39.0	35.0	41.0	32.0	41.0
23	37.188560179380524	39.0	35.0	41.0	32.0	41.0
24	37.05083470986659	39.0	35.0	41.0	32.0	41.0
25	36.94631686137149	39.0	35.0	41.0	32.0	41.0
26	36.82988039086911	38.0	35.0	41.0	31.0	41.0
27	36.72145600732382	38.0	35.0	41.0	31.0	41.0
28	36.74214215110687	38.0	35.0	41.0	31.0	41.0
29	36.69244764198194	38.0	35.0	41.0	31.0	41.0
30	36.61603760755202	38.0	35.0	41.0	31.0	41.0
31	36.503875853284775	38.0	35.0	41.0	31.0	41.0
32	36.423333731367045	38.0	35.0	41.0	30.0	41.0
33	36.274349712420644	38.0	35.0	41.0	30.0	41.0
34	36.06777684903245	38.0	35.0	41.0	30.0	41.0
35	35.904716533657066	38.0	35.0	41.0	29.0	41.0
36	35.78951894308781	38.0	35.0	41.0	29.0	41.0
37	35.76749855712779	38.0	35.0	41.0	29.0	41.0
38	35.660561824586544	38.0	35.0	40.0	29.0	41.0
39	35.55870997273469	38.0	35.0	40.0	28.0	41.0
40	35.404576392620456	38.0	35.0	40.0	27.0	41.0
41	35.303732063605786	38.0	34.0	40.0	27.0	41.0
42	35.230588393336916	38.0	34.0	40.0	27.0	41.0
43	35.145152944454395	37.0	34.0	40.0	27.0	41.0
44	35.02526353148778	37.0	34.0	40.0	26.0	41.0
45	34.90621911092536	37.0	34.0	40.0	26.0	41.0
46	34.82223897280766	37.0	34.0	40.0	26.0	41.0
47	34.74426665605243	37.0	34.0	40.0	25.0	41.0
48	34.65605906820308	36.0	34.0	40.0	25.0	41.0
49	34.57425733542964	36.0	34.0	40.0	26.0	41.0
50	34.49298299732654	36.0	34.0	40.0	25.0	41.0
51	34.13445164222076	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	0.0
12	4.0
13	4.0
14	19.0
15	35.0
16	87.0
17	220.0
18	466.0
19	857.0
20	1541.0
21	2387.0
22	3780.0
23	5464.0
24	8351.0
25	12231.0
26	16720.0
27	19609.0
28	21088.0
29	23274.0
30	27203.0
31	32750.0
32	40922.0
33	53732.0
34	89888.0
35	125576.0
36	101022.0
37	131464.0
38	185554.0
39	301480.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.12848859965106	23.146572598032385	27.54584021599963	15.179098586316927
2	33.75574661173801	24.763335787874567	27.972150907848565	13.50876669253886
3	29.09601568252831	25.4972104470582	31.66598669240618	13.740787178007311
4	26.35124153349122	28.266530008425043	30.072110441087695	15.310118016996038
5	23.204702104934956	31.706868071725676	29.918369919265498	15.170059904073874
6	22.000898892802887	40.021875269502	27.531826112338383	10.445399725356738
7	86.72764874851566	3.5030283731698737	7.981985657518526	1.7873372207959346
8	84.12915198917348	5.828540344033806	7.667290252817748	2.375017413974964
9	81.26828467371186	5.063154682534944	10.029039876344193	3.639520767409
10	43.07678402027319	27.395002023338044	15.974502623705561	13.55371133268321
11	33.95932427143246	25.235005738319373	24.215376042350787	16.590293947897386
12	29.645799749238762	22.25058212430593	29.065250993425813	19.038367133029503
13	24.43794322712467	26.543375723923816	30.496099269608134	18.522581779343376
14	21.127380739148606	27.648582668285336	31.882251676717015	19.34178491584904
15	20.73946371591007	23.133802349725688	36.84332729648868	19.28340663787556
16	24.04803603531886	21.780404800286586	34.30967686296363	19.86188230143093
17	23.761866380082395	21.945008325538506	29.736020724288682	24.55710457009042
18	25.374566309099716	22.34229572578131	31.43935624680744	20.84378171831154
19	27.805225519268152	24.523354628137003	27.125582953542832	20.545836899052016
20	29.160530313584225	24.41580260181371	26.488977783084895	19.93468930151717
21	27.05493196940448	25.135994852097305	27.9063924214381	19.902680757060125
22	26.05951599100444	22.714871866313743	27.985667469367986	23.239944673313833
23	24.35692678169841	25.07081683151896	28.022402664172326	22.549853722610305
24	24.012627619559375	23.62031564073477	31.68141049880258	20.68564624090327
25	24.23718497290054	24.734561267339345	28.76971096118508	22.25854279857504
26	22.311779807749716	26.458544788743605	28.189245129062428	23.040430274444248
27	22.08813461500189	25.446792843353833	29.308797871846416	23.156274669797867
28	21.755610616885917	26.04798959805229	30.640303567045464	21.556096218016332
29	22.81288766825217	24.322181755461354	30.608212098898107	22.256718477388368
30	24.009725290398766	24.8872237811876	30.46939784133049	20.633653087083143
31	26.36633364512641	24.760599306094562	27.694688240093935	21.178378808685096
32	27.652563005419893	24.14207150012273	27.174590854512044	21.03077463994534
33	26.684511844819923	24.42732899476586	26.556394743301425	22.331764417112794
34	23.91784584154278	25.38542931252944	29.15936938191998	21.537355464007803
35	23.552401138376418	26.245845523115808	29.019808811139637	21.181944527368135
36	26.675058544125353	26.45199381720965	26.670912359610195	20.202035279054805
37	24.395817992450628	27.530084714842012	26.980964037653987	21.09313325505337
38	24.622780132810583	27.35055492533551	26.856246807437923	21.170418134415986
39	24.38454037056939	26.88079221976768	26.1892086426387	22.545458767024236
40	25.738186691079402	24.49963845271028	27.688717734392103	22.07345712181822
41	22.01068388825867	24.724527500812652	28.41819743798966	24.846591172939014
42	23.70672212603074	25.531457931153433	26.734846524833987	24.026973417981836
43	22.92674189503851	24.399300787443362	28.549548563429987	24.12440875408814
44	23.05411268334428	25.60476247338149	28.285519533504488	23.05560530976974
45	22.76222129347689	26.26699106414313	26.943980071778746	24.026807570601232
46	23.981862930456877	25.855855407619693	27.337370058577292	22.824911603346138
47	23.182146861172477	25.59414824102268	28.740107203746824	22.48359769405802
48	23.164318267757277	24.862263750406328	29.40532104735938	22.568096934477015
49	23.28513808452909	24.77262324118853	28.9752787894468	22.96695988483558
50	22.730710291161664	26.43988695842538	28.599634472373143	22.229768278039817
51	22.17213631327907	26.424628999409585	28.119423381827108	23.283811305484242
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1029.0
1	1579.5
2	2130.0
3	9120.5
4	16111.0
5	10980.5
6	5850.0
7	5820.5
8	5791.0
9	6063.0
10	6335.0
11	6478.5
12	6622.0
13	6570.5
14	6519.0
15	6350.0
16	6181.0
17	5966.0
18	5751.0
19	5665.0
20	5579.0
21	5568.5
22	5558.0
23	5781.0
24	6004.0
25	6710.5
26	8451.5
27	9486.0
28	11140.5
29	12795.0
30	15084.0
31	17373.0
32	19570.0
33	21767.0
34	25084.5
35	28402.0
36	32607.5
37	36813.0
38	39125.5
39	41438.0
40	47873.5
41	54309.0
42	59558.5
43	64808.0
44	68895.0
45	72982.0
46	76964.0
47	80946.0
48	84059.5
49	87173.0
50	85538.0
51	83903.0
52	80501.5
53	77100.0
54	73278.5
55	69457.0
56	67594.0
57	65731.0
58	63701.0
59	61671.0
60	59230.5
61	56790.0
62	52321.5
63	47853.0
64	42978.0
65	38103.0
66	33365.0
67	28627.0
68	24339.5
69	20052.0
70	17559.0
71	15066.0
72	12800.0
73	10534.0
74	8784.0
75	5659.0
76	4284.0
77	3254.0
78	2224.0
79	1747.0
80	1270.0
81	992.5
82	715.0
83	452.0
84	189.0
85	136.0
86	83.0
87	65.5
88	48.0
89	28.0
90	8.0
91	8.5
92	9.0
93	6.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1205928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.792725269604286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93191154603156	20.5476930625554
2	6.268299841599184	3.4842627081014035
3	2.8372843117660005	2.3656759016601216
4	1.9079592144211805	2.1210954508807154
5	1.407379023256947	1.9557449271792173
6	1.1458935732356856	1.9108503161486754
7	1.0114663776013886	1.9677985006482057
8	0.8770619563354796	1.9500753597482923
9	0.7967062768685156	1.9928374806218347
>10	9.597589119248017	56.04440384037436
>50	0.19022265906807378	3.219573039552019
>100	0.02607846248156245	1.3226166618684656
>500	0.0015340272045520906	0.23440611199387978
>1k	3.0680544091041815E-4	0.08637809801738822
>5k	3.0680544091041815E-4	0.7965885406499909
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9342	0.7746731148128246	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.292369030323536E-5	0.0	0.0	0.032589010289171494	0.0
2	8.292369030323536E-5	0.0	0.0	0.13483392043306067	0.0
3	8.292369030323536E-5	0.0	0.0	0.23326434082300104	0.0
4	8.292369030323536E-5	0.0	0.0	0.3869219389548961	0.0
5	8.292369030323536E-5	0.0	0.0	0.6626432092131537	0.0
6	8.292369030323536E-5	0.0	0.0	1.2065396939120743	0.0
7	8.292369030323536E-5	0.0	0.0	1.4427063638956887	0.0
8	8.292369030323536E-5	0.0	0.0	1.914625085411401	0.0
9	8.292369030323536E-5	0.0	0.0	2.201209359099382	0.0
10	8.292369030323536E-5	0.0	0.0	2.6324954723665095	0.0
11	8.292369030323536E-5	0.0	0.0	2.997774328152261	0.0
12	8.292369030323536E-5	0.0	0.0	3.2941435972960242	0.0
13	8.292369030323536E-5	0.0	0.0	3.4496255166145904	0.0
14	8.292369030323536E-5	0.0	0.0	3.533958909652981	0.0
15	8.292369030323536E-5	0.0	0.0	3.613482728653784	0.0
16	8.292369030323536E-5	0.0	0.0	3.7536237652662514	0.0
17	8.292369030323536E-5	0.0	0.0	3.901145010315707	0.0
18	8.292369030323536E-5	0.0	0.0	4.088469626710716	0.0
19	8.292369030323536E-5	0.0	0.0	4.1920413158994565	0.0
20	8.292369030323536E-5	0.0	0.0	4.30514922947307	0.0
21	8.292369030323536E-5	0.0	0.0	4.444461029182505	0.0
22	8.292369030323536E-5	0.0	0.0	4.598201551004704	0.0
23	8.292369030323536E-5	0.0	0.0	4.766453718629968	0.0
24	8.292369030323536E-5	0.0	0.0	4.903609502391519	0.0
25	8.292369030323536E-5	0.0	0.0	5.017214958106952	0.0
26	8.292369030323536E-5	0.0	0.0	5.129327787396926	0.0
27	8.292369030323536E-5	0.0	0.0	5.2562839572511795	0.0
28	8.292369030323536E-5	0.0	0.0	5.3740355974817735	0.0
29	8.292369030323536E-5	0.0	0.0	5.507791509940892	0.0
30	8.292369030323536E-5	0.0	0.0	5.688233460040732	0.0
31	8.292369030323536E-5	0.0	0.0	5.832354833787755	0.0
32	8.292369030323536E-5	0.0	0.0	5.984353958113585	0.0
33	8.292369030323536E-5	0.0	0.0	6.129885034595763	0.0
34	8.292369030323536E-5	0.0	0.0	6.280391532496136	0.0
35	8.292369030323536E-5	0.0	0.0	6.455277595345659	0.0
36	8.292369030323536E-5	0.0	0.0	6.607193795981186	0.0
37	8.292369030323536E-5	0.0	0.0	6.752724872463364	0.0
38	8.292369030323536E-5	0.0	0.0	6.935405762201392	0.0
39	8.292369030323536E-5	0.0	0.0	7.200678647481442	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGT	20	7.033425E-4	45.0	15
CGTTTTT	5165	0.0	40.68732	1
ACGGGAT	300	0.0	39.0	5
AGGGCGA	1485	0.0	38.636364	6
GTATGCG	35	6.2485433E-6	38.57143	1
AGTACGG	70	0.0	38.57143	2
GCGTTAG	100	0.0	38.25	1
GTTGATC	655	0.0	38.129772	16
TTGATCC	675	0.0	38.000004	17
AAGGGAT	1150	0.0	37.369564	5
TAGGGAT	1260	0.0	36.785713	5
GTAGCAT	655	0.0	36.755726	29
GGGCGAT	2955	0.0	36.47208	7
GCCAGTA	655	0.0	36.412212	25
AGGGATC	885	0.0	36.355934	6
CATATGC	665	0.0	36.203007	33
GACCGAT	885	0.0	36.101696	9
GCTAACG	25	0.0021071495	36.0	1
TAAGGGA	1045	0.0	35.956936	4
ATGGGAC	695	0.0	35.935253	5
>>END_MODULE