FastQCFastQC Report
Thu 26 May 2016
SRR1547129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547129_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences609433
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44830.7356017806715422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT25750.040.7184451
AAATGCG451.925764E-840.01
ACAACGA1250.039.613
GGGCGAT14600.039.4520577
GCGATAA800.039.3759
ATAGGGC3400.039.044124
AATGCGG750.039.02
CGTCATA1500.039.038
AGGGCGA7600.038.7828946
TACGCGG356.243459E-638.5714262
GCGTAAG700.038.5714261
ACGGGAC1300.038.0769275
CGGTCTA659.094947E-1238.07692731
TACGGGA1250.037.84
TATACTA2800.037.76785744
TAGGGAC4500.037.55
CGACGGT601.546141E-1037.49999628
TTAACGG601.546141E-1037.4999962
ATTCTCG301.1392928E-437.49999630
GCGATTG1750.037.2857139
Produced by FastQC (version 0.11.5)