##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547129_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	609433
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.482983363224506	31.0	31.0	33.0	30.0	34.0
2	31.84787006939237	31.0	31.0	34.0	30.0	34.0
3	31.94345235653468	33.0	31.0	34.0	30.0	34.0
4	35.71072291785971	37.0	35.0	37.0	33.0	37.0
5	35.661426276555424	37.0	35.0	37.0	33.0	37.0
6	35.69520193360058	37.0	35.0	37.0	33.0	37.0
7	36.02394848982579	37.0	35.0	37.0	35.0	37.0
8	34.70037231328136	37.0	35.0	37.0	33.0	37.0
9	37.01283980355511	39.0	37.0	39.0	34.0	39.0
10	36.939952381968155	39.0	37.0	39.0	32.0	39.0
11	36.84590923038299	39.0	37.0	39.0	32.0	39.0
12	36.556254748266014	39.0	35.0	39.0	32.0	39.0
13	36.478754842616006	39.0	35.0	39.0	32.0	39.0
14	37.56018627150154	40.0	36.0	41.0	32.0	41.0
15	37.72409436312113	40.0	36.0	41.0	33.0	41.0
16	37.75059932757169	40.0	36.0	41.0	33.0	41.0
17	37.64156519256424	40.0	36.0	41.0	33.0	41.0
18	37.55455316663193	39.0	36.0	41.0	32.0	41.0
19	37.489842197583656	39.0	36.0	41.0	32.0	41.0
20	37.360840322069855	39.0	35.0	41.0	32.0	41.0
21	37.25388188693425	39.0	35.0	41.0	32.0	41.0
22	37.168089027013636	39.0	35.0	41.0	32.0	41.0
23	37.08322982181799	39.0	35.0	41.0	32.0	41.0
24	36.93328552933628	39.0	35.0	41.0	31.0	41.0
25	36.83509754148528	38.0	35.0	41.0	31.0	41.0
26	36.70898359622797	38.0	35.0	41.0	31.0	41.0
27	36.60327550362386	38.0	35.0	41.0	31.0	41.0
28	36.63090938626559	38.0	35.0	41.0	31.0	41.0
29	36.569089629212726	38.0	35.0	41.0	31.0	41.0
30	36.49840589531581	38.0	35.0	41.0	31.0	41.0
31	36.393395172233866	38.0	35.0	41.0	30.0	41.0
32	36.29314789320565	38.0	35.0	41.0	30.0	41.0
33	36.12987974067699	38.0	35.0	41.0	30.0	41.0
34	35.94717713021776	38.0	35.0	41.0	29.0	41.0
35	35.81975869373664	38.0	35.0	41.0	29.0	41.0
36	35.69331329284761	38.0	35.0	41.0	28.0	41.0
37	35.64947418338029	38.0	35.0	41.0	28.0	41.0
38	35.52737052309278	38.0	35.0	40.0	27.0	41.0
39	35.43652050348439	38.0	35.0	40.0	27.0	41.0
40	35.290358415117005	38.0	34.0	40.0	27.0	41.0
41	35.17297225453824	38.0	34.0	40.0	26.0	41.0
42	35.110775097508665	38.0	34.0	40.0	26.0	41.0
43	35.023790966357254	37.0	34.0	40.0	26.0	41.0
44	34.89678931071997	37.0	34.0	40.0	26.0	41.0
45	34.75986367656494	37.0	34.0	40.0	25.0	41.0
46	34.68165819704545	37.0	34.0	40.0	25.0	41.0
47	34.61626626716965	36.0	34.0	40.0	24.0	41.0
48	34.52107122522082	36.0	34.0	40.0	24.0	41.0
49	34.4378610938364	36.0	34.0	40.0	24.0	41.0
50	34.3365948348711	36.0	34.0	40.0	24.0	41.0
51	33.97170648783377	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	2.0
13	7.0
14	6.0
15	15.0
16	51.0
17	111.0
18	267.0
19	495.0
20	883.0
21	1336.0
22	2052.0
23	3041.0
24	4438.0
25	6648.0
26	8934.0
27	10391.0
28	11221.0
29	12389.0
30	14491.0
31	17336.0
32	20935.0
33	27560.0
34	45206.0
35	63333.0
36	50714.0
37	65868.0
38	92591.0
39	149017.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.50529754706424	23.69825723254238	26.121165082954157	14.675280137439225
2	34.19293014982779	25.34716695682708	27.035621635192054	13.42428125815307
3	30.155078573034277	25.40689460531346	30.468976901480556	13.9690499201717
4	26.15759238505299	27.78861663218106	30.226292307768038	15.827498674997909
5	23.49511759291013	31.54046466141479	29.416851401220477	15.5475663444546
6	22.922290063058615	40.08562056862691	26.749618087632278	10.242471280682208
7	86.42098475140007	3.8375014152499123	7.935080640529804	1.8064331928202115
8	83.71322196205325	6.123068491532293	7.764594303229395	2.399115243185059
9	80.66399423726645	5.540723918790088	10.113663027765153	3.6816188161783163
10	42.26239799945195	27.853430976005566	16.32829203538371	13.555878989158776
11	34.42199552698984	25.699789804621677	23.563705936501634	16.31450873188685
12	30.104375706599413	22.682394947434748	28.816129090482466	18.397100255483377
13	24.486695009951873	26.971299552206723	30.179527528046563	18.36247790979484
14	21.555609886566693	29.101476290256684	30.63011028283667	18.712803540339955
15	21.13242965182391	23.23635247845128	36.52148800606466	19.109729863660156
16	24.200855549338485	22.37981861828946	34.289577361252185	19.129748471119875
17	24.311121977313338	22.3474934898504	29.276885235948825	24.064499296887433
18	25.469903992727666	22.111536460939924	31.60757622248877	20.810983323843637
19	28.158140435453937	24.557088309953677	27.191832408156436	20.09293884643595
20	29.932576673727873	24.07795442649151	26.523670362451657	19.46579853732896
21	27.747923069476055	24.811423076859967	28.287276862263777	19.153376991400204
22	26.51841958016714	22.67730825209662	27.466842130308006	23.337430037428234
23	24.614354654244192	26.364998285291406	27.08451954521662	21.93612751524778
24	23.97457965026508	24.151629465421138	31.802675601747854	20.071115282565927
25	24.44321196915822	24.836364292711423	29.071940639906273	21.648483098224087
26	22.514698088222985	26.77751286851877	28.00275009722152	22.705038946036726
27	22.293837058380493	25.901780835629186	28.878482130111106	22.92589997587922
28	21.708210746710467	26.429484455223136	30.548723157426654	21.313581640639743
29	23.03288466492625	24.61960543652871	30.126035183523047	22.221474715021998
30	23.616870107132367	25.86978388108291	29.85922980869103	20.654116203093693
31	26.139870994842745	25.74261649762977	26.979339812579887	21.1381726949476
32	26.61112870487814	25.657783546345534	26.600627140309108	21.130460608467214
33	26.135112473397403	25.69519537012272	26.425546368509746	21.74414578797013
34	22.441843484025313	26.940287119338794	28.46711615550848	22.150753241127408
35	23.107216051641444	26.92272981607494	28.27037590678549	21.699678225498126
36	26.103771866636695	27.273219533566444	24.995692717657235	21.627315882139627
37	23.869399917628353	28.55900484548753	25.980706656843328	21.590888580040794
38	23.754703142100936	28.21983712729701	25.766737278749265	22.25872245185279
39	23.643452192447732	27.34886361585277	25.517981468020274	23.489702723679223
40	25.569668856133486	24.789927686882727	26.76290913028996	22.877494326693828
41	21.538380757195622	25.83270023119851	27.407114481821626	25.221804529784244
42	23.876947917162347	26.391744457553166	25.97332274425573	23.75798488102876
43	23.13494674558155	25.32222574097563	27.578913514693166	23.963913998749657
44	22.945426322499767	26.357450285757416	27.8119169785686	22.885206413174213
45	22.259378799638352	27.614848555952825	26.534664187859864	23.591108456548955
46	23.160052048379395	26.67085635336452	27.110281195800027	23.058810402456054
47	22.973813364225435	26.653463137047055	27.950898622161912	22.421824876565594
48	23.09244822646624	25.880613619544725	28.523397978120645	22.503540175868388
49	23.638693671002393	25.37801530274862	28.289902253406034	22.693388772842955
50	21.991260729235208	27.18723797365748	28.493205979984676	22.328295317122638
51	22.265285929708433	27.231541449183094	27.311287705129196	23.19188491597928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	504.0
1	794.0
2	1084.0
3	4477.5
4	7871.0
5	5443.0
6	3015.0
7	3003.5
8	2992.0
9	3149.0
10	3306.0
11	3325.5
12	3345.0
13	3358.5
14	3372.0
15	3228.0
16	3084.0
17	3029.5
18	2975.0
19	3004.0
20	3033.0
21	3043.5
22	3054.0
23	3115.5
24	3177.0
25	3566.5
26	4228.5
27	4501.0
28	5374.5
29	6248.0
30	7208.5
31	8169.0
32	9745.0
33	11321.0
34	12765.0
35	14209.0
36	15463.0
37	16717.0
38	18996.5
39	21276.0
40	24538.5
41	27801.0
42	30763.5
43	33726.0
44	35814.5
45	37903.0
46	39745.0
47	41587.0
48	42528.5
49	43470.0
50	43365.5
51	43261.0
52	41580.5
53	39900.0
54	38160.5
55	36421.0
56	34995.0
57	33569.0
58	32264.0
59	30959.0
60	29066.5
61	27174.0
62	25177.0
63	23180.0
64	20962.5
65	18745.0
66	16167.0
67	13589.0
68	12113.0
69	10637.0
70	9111.5
71	7586.0
72	6305.5
73	5025.0
74	4158.5
75	2744.0
76	2196.0
77	1686.5
78	1177.0
79	849.0
80	521.0
81	399.0
82	277.0
83	213.5
84	150.0
85	105.5
86	61.0
87	35.0
88	9.0
89	7.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	609433.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.65465836304134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2476327844262	23.641600821722463
2	6.991508579532117	4.705936653728517
3	3.811101249474919	3.847839316141151
4	2.643869560430709	3.559141072509591
5	2.161602636285073	3.6373999120411815
6	1.7911898144165515	3.6169128760529072
7	1.5126023423582193	3.5634280549841377
8	1.3685340290581125	3.684603616491781
9	1.1959930174171924	3.62256627651827
>10	8.222850016683743	43.64592613793551
>50	0.03708096013106759	0.8447252362573001
>100	0.015533915730312951	0.8739002144592197
>500	0.0	0.0
>1k	5.010940558165468E-4	0.7560198111579857
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4483	0.7356017806715422	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03609912820605382	0.0
2	0.0	0.0	0.0	0.1096100801892907	0.0
3	0.0	0.0	0.0	0.18361329301170104	0.0
4	0.0	0.0	0.0	0.3211181540874879	0.0
5	0.0	0.0	0.0	0.5542857049093174	0.0
6	0.0	0.0	0.0	0.9983049818437794	0.0
7	0.0	0.0	0.0	1.2047263604038507	0.0
8	0.0	0.0	0.0	1.6818912005093258	0.0
9	0.0	0.0	0.0	1.9288420548280123	0.0
10	0.0	0.0	0.0	2.3016475970287136	0.0
11	0.0	0.0	0.0	2.6020907958709163	0.0
12	0.0	0.0	0.0	2.833945651121616	0.0
13	0.0	0.0	0.0	2.975552685857182	0.0
14	0.0	0.0	0.0	3.0434846816631196	0.0
15	0.0	0.0	0.0	3.11748789448553	0.0
16	0.0	0.0	0.0	3.2398967564933305	0.0
17	0.0	0.0	0.0	3.375432574212424	0.0
18	1.6408694639115373E-4	0.0	0.0	3.55412325883239	0.0
19	1.6408694639115373E-4	0.0	0.0	3.6522472527743	0.0
20	1.6408694639115373E-4	0.0	0.0	3.7492226380914717	0.0
21	1.6408694639115373E-4	0.0	0.0	3.87409280429514	0.0
22	1.6408694639115373E-4	0.0	0.0	4.013730795674012	0.0
23	1.6408694639115373E-4	0.0	0.0	4.167316177496132	0.0
24	1.6408694639115373E-4	0.0	0.0	4.298093473769881	0.0
25	1.6408694639115373E-4	0.0	0.0	4.421486857456029	0.0
26	3.2817389278230746E-4	0.0	0.0	4.531917372377276	0.0
27	3.2817389278230746E-4	0.0	0.0	4.648747278207777	0.0
28	3.2817389278230746E-4	0.0	0.0	4.757044662825939	0.0
29	3.2817389278230746E-4	0.0	0.0	4.89241639359864	0.0
30	3.2817389278230746E-4	0.0	0.0	5.065528122041307	0.0
31	3.2817389278230746E-4	0.0	0.0	5.211893678222216	0.0
32	3.2817389278230746E-4	0.0	0.0	5.359736016920646	0.0
33	3.2817389278230746E-4	0.0	0.0	5.49543592158613	0.0
34	3.2817389278230746E-4	0.0	0.0	5.649185390354641	0.0
35	3.2817389278230746E-4	0.0	0.0	5.825907031617914	0.0
36	3.2817389278230746E-4	0.0	0.0	5.971780326959649	0.0
37	3.2817389278230746E-4	0.0	0.0	6.122412143746729	0.0
38	3.2817389278230746E-4	0.0	0.0	6.3011028283666946	0.0
39	3.2817389278230746E-4	0.0	0.0	6.555437595272983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	2575	0.0	40.718445	1
AAATGCG	45	1.925764E-8	40.0	1
ACAACGA	125	0.0	39.6	13
GGGCGAT	1460	0.0	39.452057	7
GCGATAA	80	0.0	39.375	9
ATAGGGC	340	0.0	39.04412	4
AATGCGG	75	0.0	39.0	2
CGTCATA	150	0.0	39.0	38
AGGGCGA	760	0.0	38.782894	6
TACGCGG	35	6.243459E-6	38.571426	2
GCGTAAG	70	0.0	38.571426	1
ACGGGAC	130	0.0	38.076927	5
CGGTCTA	65	9.094947E-12	38.076927	31
TACGGGA	125	0.0	37.8	4
TATACTA	280	0.0	37.767857	44
TAGGGAC	450	0.0	37.5	5
CGACGGT	60	1.546141E-10	37.499996	28
TTAACGG	60	1.546141E-10	37.499996	2
ATTCTCG	30	1.1392928E-4	37.499996	30
GCGATTG	175	0.0	37.285713	9
>>END_MODULE