FastQCFastQC Report
Thu 26 May 2016
SRR1547128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547128_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences802368
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44140.5501216399457606No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10140.12637592725532426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA750.045.031
GTCGGTA207.0320116E-445.045
CGACGGT800.042.187528
GCGTTAG556.002665E-1140.9090921
ACGTTAG501.0804797E-940.51
CACGACG950.040.26315726
TCGTAAG900.040.01
CGTTTTT23300.039.8819731
ATATCAC850.039.70588330
CGGTAGT1550.039.1935512
TAGTACA356.2459294E-638.5714313
ACGCCGG1650.038.18181627
TAGCATA4200.038.03571330
GTAGCAT4500.038.029
AGGGTAC2850.037.8947376
CGTAAGG2100.037.52
CTATGCG301.139615E-437.4999961
ATCGGGT301.139615E-437.4999964
GTTGATC4650.037.25806416
CGTTAGG1150.037.1739122
Produced by FastQC (version 0.11.5)