FastQCFastQC Report
Thu 26 May 2016
SRR1547127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547127_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences852017
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51650.6062085615662598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA302.1648375E-645.00000431
CTCACGA800.045.024
ATCTAGT253.8899525E-545.029
ATCGAAC253.8899525E-545.021
CGAATAT1250.043.214
TCACGAC750.042.025
CGGTCTA750.042.031
CGTTTTT29050.041.127371
TCGTTAG501.0804797E-940.51
TACGGGA2100.039.6428574
ATAACGC750.039.011
TATACTA7050.038.9361744
AAGTACG356.246388E-638.5714261
TTAACGG700.038.5714262
ATACCGG356.246388E-638.5714262
ATACTAT7150.038.39160545
TAACGGG1650.038.1818163
ATTATAC7200.038.12542
CAACCCG659.094947E-1238.07692723
ACGTAGG950.037.8947372
Produced by FastQC (version 0.11.5)