##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547127_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	852017
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.379560501727077	31.0	31.0	33.0	30.0	34.0
2	31.75032305693431	31.0	31.0	34.0	30.0	34.0
3	31.87314689730369	31.0	31.0	34.0	30.0	34.0
4	35.64034050963772	37.0	35.0	37.0	33.0	37.0
5	35.56507557947788	37.0	35.0	37.0	33.0	37.0
6	35.60971670753048	37.0	35.0	37.0	33.0	37.0
7	35.95605604113533	37.0	35.0	37.0	35.0	37.0
8	34.61802522719617	37.0	35.0	37.0	33.0	37.0
9	36.92397921637714	39.0	37.0	39.0	33.0	39.0
10	36.84184939971855	39.0	37.0	39.0	32.0	39.0
11	36.7275887687687	39.0	35.0	39.0	32.0	39.0
12	36.37134822427252	38.0	35.0	39.0	32.0	39.0
13	36.25590686570808	39.0	35.0	39.0	32.0	39.0
14	37.30267353820405	39.0	35.0	41.0	32.0	41.0
15	37.49367207461823	40.0	35.0	41.0	32.0	41.0
16	37.54743039164711	40.0	35.0	41.0	32.0	41.0
17	37.45916689455727	39.0	35.0	41.0	32.0	41.0
18	37.38713781532528	39.0	35.0	41.0	32.0	41.0
19	37.31873894534968	39.0	35.0	41.0	32.0	41.0
20	37.19268864353646	39.0	35.0	41.0	32.0	41.0
21	37.08911207170749	39.0	35.0	41.0	32.0	41.0
22	37.003930672744794	39.0	35.0	41.0	31.0	41.0
23	36.92446394848929	39.0	35.0	41.0	31.0	41.0
24	36.75919846669726	38.0	35.0	41.0	31.0	41.0
25	36.647321591001116	38.0	35.0	41.0	31.0	41.0
26	36.49904872790097	38.0	35.0	41.0	31.0	41.0
27	36.38652749886446	38.0	35.0	40.0	30.0	41.0
28	36.409233618578035	38.0	35.0	40.0	30.0	41.0
29	36.36021699097553	38.0	35.0	40.0	30.0	41.0
30	36.28409879145604	38.0	35.0	40.0	30.0	41.0
31	36.17601644098651	38.0	35.0	40.0	30.0	41.0
32	36.103434555883275	38.0	35.0	40.0	30.0	41.0
33	35.971493526537614	38.0	35.0	41.0	30.0	41.0
34	35.79343135172186	38.0	35.0	40.0	29.0	41.0
35	35.65048115237137	38.0	35.0	40.0	28.0	41.0
36	35.51981826653694	38.0	35.0	40.0	28.0	41.0
37	35.45689698679721	38.0	34.0	40.0	27.0	41.0
38	35.36317233106851	38.0	34.0	40.0	27.0	41.0
39	35.250166369919846	38.0	34.0	40.0	27.0	41.0
40	35.087775243921186	37.0	34.0	40.0	26.0	41.0
41	34.98062832079641	37.0	34.0	40.0	26.0	41.0
42	34.919687048497856	37.0	34.0	40.0	26.0	41.0
43	34.8245821386193	37.0	34.0	40.0	26.0	41.0
44	34.67817895652317	36.0	34.0	40.0	25.0	41.0
45	34.55259460785407	36.0	34.0	40.0	24.0	41.0
46	34.48153381916089	36.0	34.0	40.0	24.0	41.0
47	34.38285151587351	36.0	33.0	40.0	24.0	41.0
48	34.30688472178372	35.0	33.0	40.0	24.0	41.0
49	34.23099539093703	36.0	33.0	40.0	24.0	41.0
50	34.12568763299324	35.0	33.0	40.0	24.0	41.0
51	33.748534360229904	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	8.0
14	13.0
15	35.0
16	92.0
17	228.0
18	440.0
19	837.0
20	1415.0
21	2107.0
22	3252.0
23	4593.0
24	6668.0
25	9451.0
26	12173.0
27	14445.0
28	16298.0
29	18162.0
30	21380.0
31	25800.0
32	31478.0
33	40900.0
34	66912.0
35	94870.0
36	73039.0
37	92919.0
38	124449.0
39	189890.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.01868742055616	23.544718004452964	28.012703971869108	14.42389060312177
2	31.883870861731634	25.41134742616638	29.024068768580907	13.680712943521081
3	30.31793966552311	25.297382563962927	30.48683300920052	13.897844761313449
4	26.074479734559286	28.35295539877725	30.185782678045157	15.386782188618303
5	23.263855063924783	32.65932487262578	29.186272104899313	14.890547958550124
6	22.14591962366948	40.87113285298298	26.355577412187785	10.627370111159754
7	86.91458034288048	3.9969859756319415	7.170044729154465	1.9183889523331106
8	84.35148594452929	6.2914237626714025	6.648576260802308	2.708514031997014
9	81.27314361098429	5.5685508622480535	9.462839356491713	3.6954661702759455
10	45.665520758388624	25.8118089193056	15.048056552862207	13.474613769443566
11	36.750909899685105	23.617134399900472	22.709406032978215	16.92254966743621
12	31.89478613689633	20.822002377886825	27.899560689516754	19.383650795700085
13	24.510778540803763	26.99981338400525	29.197304748614172	19.292103326576818
14	21.22997545823616	28.444385499350368	30.48636353499989	19.839275507413586
15	20.759327572102436	22.02702528235939	36.955248545510244	20.25839860002793
16	23.173011806102462	19.586815756023647	35.37546786038307	21.864704577490826
17	23.688494478396557	20.754515461545957	28.068454033194172	27.488536026863315
18	25.573081288284154	21.41846934978997	31.193274312601744	21.815175049324136
19	28.778416393100137	24.8326031053371	24.84164048369927	21.54734001786349
20	29.650934194974983	23.345543574834775	25.19668034792733	21.806841882262912
21	26.7705926055466	24.945159544938658	26.834323728282417	21.44992412123232
22	26.442312770754572	22.254485532565667	26.31097736312773	24.99222433355203
23	24.333199924414654	25.458412214779752	25.48388119016405	24.72450667064155
24	24.03578801831419	23.472418977555613	31.209823278174024	21.281969725956174
25	24.287074084202544	24.016187470437796	28.24450685843123	23.45223158692843
26	22.211059169007193	25.94502222373497	27.06553977209375	24.778378835164087
27	22.286175041108336	24.97778800188259	27.632547237907225	25.10348971910185
28	21.7204586293466	24.875325257594625	30.09705205412568	23.307164058933097
29	22.483471573923993	23.219372383414886	29.699172669089936	24.597983373571182
30	23.994004814457927	24.63788868062492	28.92712234615037	22.440984158766785
31	27.437011233343934	24.997858023959616	25.028491215550865	22.536639527145585
32	27.98746973358513	24.050928561284575	24.57497913774021	23.386622567390088
33	26.6894909373874	25.424844809434553	24.416648963577018	23.46901528960103
34	23.52875588163147	25.44186324920747	26.735616777599507	24.293764091561552
35	23.384979407687876	26.273654164177472	27.247461024838703	23.093905403295945
36	27.667523065854322	25.6672108655109	24.199869251435125	22.465396817199657
37	23.84259938475406	28.32701694919233	25.450431153369006	22.379952512684607
38	24.48155377181441	27.827731136820038	24.959595876608095	22.731119214757452
39	23.62206387900711	27.066948194695645	24.34657993913267	24.964407987164574
40	25.678360877775912	24.56793702473073	26.099948709943583	23.653753387549777
41	22.2857055669077	23.453522640980168	27.038427637007246	27.22234415510489
42	24.132147597993935	25.024970159046124	24.796336223338265	26.046546019621676
43	23.435799989906307	23.408453117719482	27.207907823435445	25.947839068938766
44	23.05716904709648	24.71007033897211	27.388655390678824	24.844105223252587
45	22.45811996708986	26.37212637776007	25.814508395959233	25.355245259190838
46	24.392236305144145	25.8271841993763	26.63115876795886	23.149420727520695
47	23.344839363533826	25.246092507543867	27.56412137316509	23.84494675575722
48	23.426645242993978	23.66678129661732	28.478891853096826	24.427681607291873
49	23.655279178701832	23.143904405663267	28.60365462191482	24.59716179372008
50	22.508588443657814	25.648901371686243	28.293214806746814	23.549295377909125
51	21.792170813493158	26.465903849336343	27.079506629562555	24.662418707607948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	762.0
1	1070.0
2	1378.0
3	5129.0
4	8880.0
5	6134.0
6	3388.0
7	3442.5
8	3497.0
9	3789.0
10	4081.0
11	4277.5
12	4474.0
13	4567.0
14	4660.0
15	4447.5
16	4235.0
17	4138.0
18	4041.0
19	3817.0
20	3593.0
21	3605.5
22	3618.0
23	3677.5
24	3737.0
25	3959.5
26	4569.0
27	4956.0
28	5633.5
29	6311.0
30	7439.0
31	8567.0
32	10003.5
33	11440.0
34	14436.0
35	17432.0
36	19447.5
37	21463.0
38	22749.5
39	24036.0
40	29523.0
41	35010.0
42	39785.5
43	44561.0
44	47232.5
45	49904.0
46	53878.0
47	57852.0
48	60597.0
49	63342.0
50	60864.0
51	58386.0
52	55613.5
53	52841.0
54	51689.5
55	50538.0
56	49589.5
57	48641.0
58	47940.0
59	47239.0
60	45353.5
61	43468.0
62	41289.0
63	39110.0
64	36134.5
65	33159.0
66	29012.0
67	24865.0
68	21930.5
69	18996.0
70	16124.0
71	13252.0
72	11111.0
73	8970.0
74	7570.5
75	4767.5
76	3364.0
77	2599.5
78	1835.0
79	1395.5
80	956.0
81	691.0
82	426.0
83	316.5
84	207.0
85	160.0
86	113.0
87	75.0
88	37.0
89	34.0
90	31.0
91	17.5
92	4.0
93	6.0
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	852017.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.783975109013554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64201825908543	22.231360139151356
2	6.4129897224162775	3.8200865253361234
3	2.741201361542812	2.4493161936295555
4	1.8553108842530253	2.2103413278429613
5	1.4014057411115648	2.0869716855447766
6	1.1518102440218447	2.058329258295207
7	1.0149226458946565	2.115990157603044
8	0.8657352175257325	2.062802893582628
9	0.7433830884501025	1.9926813062573527
>10	9.025285808818106	54.52819163536883
>50	0.1207334180831855	2.2056193566912556
>100	0.02276454988135877	1.258209149082198
>500	0.00203254909654989	0.35474911535901077
>1k	0.0	0.0
>5k	4.065098193099779E-4	0.6253512562557306
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5165	0.6062085615662598	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04025741270420661	0.0
2	0.0	0.0	0.0	0.1363822552836387	0.0
3	0.0	0.0	0.0	0.22253077109963768	0.0
4	0.0	0.0	0.0	0.408677291650284	0.0
5	0.0	0.0	0.0	0.6874275982756213	0.0
6	0.0	0.0	0.0	1.3211004005788618	0.0
7	0.0	0.0	0.0	1.622033363184068	0.0
8	0.0	0.0	0.0	2.373426821295819	0.0
9	0.0	0.0	0.0	2.7484193390507468	0.0
10	0.0	0.0	0.0	3.275756234910806	0.0
11	0.0	0.0	0.0	3.6680019295389648	0.0
12	0.0	0.0	0.0	3.9900612311726174	0.0
13	0.0	0.0	0.0	4.168813533063307	0.0
14	0.0	0.0	0.0	4.248741515720931	0.0
15	0.0	0.0	0.0	4.325970021724919	0.0
16	0.0	0.0	0.0	4.455897006749865	0.0
17	0.0	0.0	0.0	4.593452947535084	0.0
18	0.0	0.0	0.0	4.813284241981087	0.0
19	0.0	0.0	0.0	4.915864354819211	0.0
20	0.0	0.0	0.0	5.024312895165237	0.0
21	0.0	0.0	0.0	5.156704619743503	0.0
22	0.0	0.0	0.0	5.305058467143261	0.0
23	0.0	0.0	0.0	5.475947076173362	0.0
24	0.0	0.0	0.0	5.617376179113797	0.0
25	0.0	0.0	0.0	5.730284724365829	0.0
26	0.0	0.0	0.0	5.84378011236865	0.0
27	0.0	0.0	0.0	5.962322348028267	0.0
28	0.0	0.0	0.0	6.078047738484091	0.0
29	0.0	0.0	0.0	6.212434728415043	0.0
30	0.0	0.0	0.0	6.391069661755576	0.0
31	0.0	0.0	0.0	6.525808757337002	0.0
32	0.0	0.0	0.0	6.658669956115899	0.0
33	0.0	0.0	0.0	6.810779597120715	0.0
34	0.0	0.0	0.0	6.951856594410675	0.0
35	0.0	0.0	0.0	7.112064665376395	0.0
36	0.0	0.0	0.0	7.251615871514301	0.0
37	0.0	0.0	0.0	7.406659726273067	0.0
38	0.0	0.0	0.0	7.584355711212335	0.0
39	0.0	0.0	0.0	7.872026027649683	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	30	2.1648375E-6	45.000004	31
CTCACGA	80	0.0	45.0	24
ATCTAGT	25	3.8899525E-5	45.0	29
ATCGAAC	25	3.8899525E-5	45.0	21
CGAATAT	125	0.0	43.2	14
TCACGAC	75	0.0	42.0	25
CGGTCTA	75	0.0	42.0	31
CGTTTTT	2905	0.0	41.12737	1
TCGTTAG	50	1.0804797E-9	40.5	1
TACGGGA	210	0.0	39.642857	4
ATAACGC	75	0.0	39.0	11
TATACTA	705	0.0	38.93617	44
AAGTACG	35	6.246388E-6	38.571426	1
TTAACGG	70	0.0	38.571426	2
ATACCGG	35	6.246388E-6	38.571426	2
ATACTAT	715	0.0	38.391605	45
TAACGGG	165	0.0	38.181816	3
ATTATAC	720	0.0	38.125	42
CAACCCG	65	9.094947E-12	38.076927	23
ACGTAGG	95	0.0	37.894737	2
>>END_MODULE