FastQCFastQC Report
Thu 26 May 2016
SRR1547126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547126_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1294437
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.491565058786175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13030.10066152311777243No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAC207.033616E-445.00000434
TCTAACG207.033616E-445.0000041
CCGGTAT253.8910795E-545.042
CGTTAGG1950.041.5384642
TCACGAC1100.040.90909225
CGTTTTT35200.040.2059671
GTATGCG900.040.01
ATAGTCG451.9286745E-840.010
AGGGCGA14200.039.4542276
ATACGAC356.2489E-638.57142637
TACGGGA3700.038.310814
GGGCGAT27200.037.720597
TTAGGGA13450.037.639414
CTCACGA1200.037.49999624
CGGTCTA1200.037.49999631
TAGGGCG5650.037.4336285
GACACGA7500.037.225
CACGACC7450.037.1476527
ATGACGG850.037.0588232
CGTATGG1950.036.923082
Produced by FastQC (version 0.11.5)