##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547126_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1294437
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.428322119964122	31.0	31.0	33.0	30.0	34.0
2	31.78623061609024	31.0	31.0	34.0	30.0	34.0
3	31.91026523500178	31.0	31.0	34.0	30.0	34.0
4	35.65009421084224	37.0	35.0	37.0	33.0	37.0
5	35.579488225382924	37.0	35.0	37.0	33.0	37.0
6	35.62139215736262	37.0	35.0	37.0	33.0	37.0
7	36.00221408998661	37.0	35.0	37.0	35.0	37.0
8	34.70048136757525	37.0	35.0	37.0	33.0	37.0
9	36.99037187595843	39.0	37.0	39.0	33.0	39.0
10	36.881962582960774	39.0	37.0	39.0	32.0	39.0
11	36.77695940397254	39.0	35.0	39.0	32.0	39.0
12	36.40699470117124	39.0	35.0	39.0	32.0	39.0
13	36.25610284625671	39.0	35.0	39.0	32.0	39.0
14	37.29808248682632	39.0	35.0	41.0	32.0	41.0
15	37.495627056395946	40.0	35.0	41.0	32.0	41.0
16	37.57428828131458	40.0	35.0	41.0	33.0	41.0
17	37.46313802834746	39.0	35.0	41.0	32.0	41.0
18	37.4263243402344	39.0	36.0	41.0	32.0	41.0
19	37.37211853493063	39.0	35.0	41.0	32.0	41.0
20	37.25850775279137	39.0	35.0	41.0	32.0	41.0
21	37.148222740851814	39.0	35.0	41.0	32.0	41.0
22	37.06358285494002	39.0	35.0	41.0	32.0	41.0
23	36.99300468079945	39.0	35.0	41.0	32.0	41.0
24	36.851317599852294	38.0	35.0	41.0	31.0	41.0
25	36.754935157137815	38.0	35.0	41.0	31.0	41.0
26	36.64412018506888	38.0	35.0	41.0	31.0	41.0
27	36.54881697602896	38.0	35.0	41.0	31.0	41.0
28	36.5751110328274	38.0	35.0	41.0	31.0	41.0
29	36.557794624226595	38.0	35.0	41.0	31.0	41.0
30	36.50172855071355	38.0	35.0	41.0	31.0	41.0
31	36.41407190925476	38.0	35.0	40.0	31.0	41.0
32	36.33011262811554	38.0	35.0	41.0	30.0	41.0
33	36.23179961635831	38.0	35.0	41.0	30.0	41.0
34	36.10709752579693	38.0	35.0	41.0	30.0	41.0
35	36.012305736007235	38.0	35.0	41.0	30.0	41.0
36	35.90677105181635	38.0	35.0	41.0	29.0	41.0
37	35.85558895488927	38.0	35.0	41.0	29.0	41.0
38	35.742944616076336	38.0	35.0	40.0	29.0	41.0
39	35.67328112530776	38.0	35.0	40.0	29.0	41.0
40	35.51453643553143	38.0	35.0	40.0	28.0	41.0
41	35.43185802012767	38.0	35.0	40.0	28.0	41.0
42	35.36142122019071	38.0	34.0	40.0	27.0	41.0
43	35.26856695227346	37.0	34.0	40.0	27.0	41.0
44	35.16277346831093	37.0	34.0	40.0	27.0	41.0
45	35.04752490851235	37.0	34.0	40.0	27.0	41.0
46	34.97960039770186	37.0	34.0	40.0	27.0	41.0
47	34.89548892684619	37.0	34.0	40.0	26.0	41.0
48	34.83691519942647	36.0	34.0	40.0	26.0	41.0
49	34.77632669647113	36.0	34.0	40.0	26.0	41.0
50	34.65576771986586	36.0	34.0	40.0	26.0	41.0
51	34.28462489870113	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	8.0
14	24.0
15	45.0
16	108.0
17	249.0
18	564.0
19	1060.0
20	1855.0
21	2917.0
22	4238.0
23	6374.0
24	9049.0
25	12812.0
26	16643.0
27	20177.0
28	22678.0
29	25958.0
30	30431.0
31	36466.0
32	45838.0
33	59216.0
34	98525.0
35	151925.0
36	100683.0
37	130530.0
38	189641.0
39	326178.0
40	240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.49368335423045	23.752488533625044	28.127517986584127	13.626310125560378
2	30.73560165539149	25.752972141556523	29.40019483373853	14.111231369313455
3	29.90234364437976	25.622799719105682	30.649772835603432	13.82508380091113
4	25.772362811013593	28.67810484403644	29.70071158349151	15.848820761458457
5	23.345207221363417	31.981780496076674	29.461688749626287	15.211323532933624
6	21.467556937881103	41.37350832833116	26.210545588545447	10.948389145242295
7	87.77553484642357	3.839893328141887	6.5649390429970715	1.8196327824374612
8	85.32427611386262	5.835278194303779	5.983527973937704	2.856917717895888
9	82.51880933564168	5.290639868915984	8.836969276990692	3.353581518451651
10	44.03675111264588	28.462335362787066	14.079093845432416	13.421819679134636
11	35.32640058959996	24.464767308103834	24.216319527331187	15.992512574965023
12	31.459082211030744	22.59816429845562	27.219787444271137	18.722966046242497
13	23.931176256550145	30.507162573381326	28.750182511779254	16.811478658289282
14	19.620576358679486	32.54125152479418	29.562891048386287	18.275281068140046
15	18.469110509047564	24.280826336082793	38.99988952726166	18.25017362760799
16	21.659377783546052	21.52935986842156	37.05804144968044	19.753220898351948
17	21.124550673381556	22.56587226724823	28.87672401206084	27.43285304730937
18	23.73734681564263	23.207232178931843	31.558353168211355	21.49706783721417
19	27.16207895787899	25.917754205110022	26.245155229648105	20.67501160736289
20	28.870234704354093	24.85173090695028	25.28929565517673	20.988738733518897
21	25.033895044718285	27.425977471286743	27.785207005053163	19.75492047894181
22	24.979585719505852	23.44447817854403	27.15867979669926	24.417256305250852
23	22.465519758783163	28.047096923218355	26.33507849358447	23.15230482441401
24	22.1174920061772	25.194428156797127	32.59030760091067	20.097772236115006
25	22.221861705127402	25.497571531098075	30.195830310783762	22.08473645299076
26	20.18738648539867	29.71948422364317	27.311564796123722	22.781564494834434
27	20.874635073008573	27.796331532550443	28.54221565051061	22.786817743930374
28	19.58635298589271	27.389513742267873	32.26066622014049	20.763467051698925
29	20.026930626983006	25.156960130156968	31.48055872939355	23.335550513466472
30	22.545322792843532	26.651973019930676	29.792952457323146	21.009751729902653
31	26.557646297193298	25.811144149927728	26.170528191020498	21.460681361858473
32	25.984655877420064	25.717589963822107	26.818454664074032	21.47929949468379
33	24.897465075550222	25.965265207963	26.73934691298225	22.397922803504535
34	20.857098491467717	26.73432542487583	29.168511097874983	23.240064985781466
35	20.52946570594011	26.48325101955522	30.217075068157044	22.770208206347625
36	25.87526469036345	25.928183449638727	26.423147669604624	21.773404190393197
37	22.859745201968114	27.985062231688374	27.89784284596315	21.25734972038037
38	22.40549366249574	29.048767919952844	26.113205972944222	22.432532444607194
39	22.823744994928298	27.297813644078467	26.184124835739397	23.694316525253836
40	23.97490182990752	25.059156992576696	27.092241646368265	23.87369953114752
41	20.423241919073696	24.55175493283953	28.171707081920555	26.853296066166216
42	22.92456102537242	26.055265725562542	26.196021899868438	24.824151349196597
43	22.337355931574884	25.45060130388733	27.83704421304397	24.374998551493814
44	22.054839285341814	27.144696883664484	27.740477134074503	23.059986696919204
45	20.641483517544692	28.690851698460413	26.4905901175569	24.177074666437996
46	23.395576609753892	26.97589762962585	26.959056331053578	22.66946942956668
47	22.42349376601565	26.304254281977418	28.26587929733158	23.006372654675353
48	22.9692136426879	24.766442862804446	29.30262345714778	22.96172003735987
49	23.005831879033124	23.998155182523366	29.736093761225924	23.259919177217586
50	21.054713361870835	26.88064386293037	28.98372033555901	23.08092243963978
51	20.77575038414384	28.367776879060163	26.975202346657273	23.881270390138727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1111.0
1	1582.5
2	2054.0
3	6559.0
4	11064.0
5	7815.5
6	4567.0
7	4624.0
8	4681.0
9	5179.0
10	5677.0
11	5742.5
12	5808.0
13	5756.0
14	5704.0
15	5689.5
16	5675.0
17	5625.5
18	5576.0
19	5397.5
20	5219.0
21	5625.0
22	6031.0
23	6549.5
24	7068.0
25	7615.5
26	8513.5
27	8864.0
28	11249.0
29	13634.0
30	16002.0
31	18370.0
32	21002.0
33	23634.0
34	27146.0
35	30658.0
36	34654.0
37	38650.0
38	42772.0
39	46894.0
40	57165.5
41	67437.0
42	77526.0
43	87615.0
44	90999.5
45	94384.0
46	99852.5
47	105321.0
48	109103.5
49	112886.0
50	108319.0
51	103752.0
52	96112.5
53	88473.0
54	81990.5
55	75508.0
56	69909.5
57	64311.0
58	60123.5
59	55936.0
60	51833.5
61	47731.0
62	42823.0
63	37915.0
64	33669.0
65	29423.0
66	25054.5
67	20686.0
68	17560.0
69	14434.0
70	12554.5
71	10675.0
72	9072.5
73	7470.0
74	6070.0
75	4169.0
76	3668.0
77	2669.0
78	1670.0
79	1242.0
80	814.0
81	553.5
82	293.0
83	221.0
84	149.0
85	102.0
86	55.0
87	43.0
88	31.0
89	20.5
90	10.0
91	9.5
92	9.0
93	7.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1294437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.316307436479875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1392541504513	19.781973809300613
2	6.537143857370456	3.3099268729937283
3	2.388784079645533	1.8142557647902482
4	1.4608574057334538	1.4793406081762615
5	0.9681020094841394	1.2254384050987213
6	0.7233802148841366	1.0987989548084207
7	0.6091360107529119	1.0794752163196137
8	0.504171348207513	1.0211005481508755
9	0.45177054690594537	1.0293445850595808
>10	6.828484910848644	42.755317841781824
>50	1.3108264693816423	21.479794900552214
>100	0.07462537149691159	2.7985446504101237
>500	0.002833874866949014	0.5155948044194262
>1k	3.148749852165571E-4	0.10386827924528637
>5k	3.148749852165571E-4	0.5072247588931368
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6363	0.491565058786175	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1303	0.10066152311777243	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8626831587786813E-4	0.0	0.0	0.05647242778134432	0.0
2	3.8626831587786813E-4	0.0	0.0	0.19297965061258293	0.0
3	3.8626831587786813E-4	0.0	0.0	0.28954672958204997	0.0
4	3.8626831587786813E-4	0.0	0.0	0.5541405259583896	0.0
5	3.8626831587786813E-4	0.0	0.0	0.9250353628643186	0.0
6	3.8626831587786813E-4	0.0	0.0	1.6204728387708325	0.0
7	3.8626831587786813E-4	0.0	0.0	1.9334274282950812	0.0
8	3.8626831587786813E-4	0.0	0.0	2.8053895245577807	0.0
9	3.8626831587786813E-4	0.0	0.0	3.1982244018055725	0.0
10	3.8626831587786813E-4	0.0	0.0	3.795395218152757	0.0
11	3.8626831587786813E-4	0.0	0.0	4.282943086453802	0.0
12	3.8626831587786813E-4	0.0	0.0	4.710542112130602	0.0
13	3.8626831587786813E-4	0.0	0.0	4.912792202324254	0.0
14	3.8626831587786813E-4	0.0	0.0	4.996921441522454	0.0
15	3.8626831587786813E-4	0.0	0.0	5.086767451795645	0.0
16	3.8626831587786813E-4	0.0	0.0	5.2748801216281676	0.0
17	3.8626831587786813E-4	0.0	0.0	5.476357675190063	0.0
18	3.8626831587786813E-4	0.0	0.0	5.748831345210312	0.0
19	3.8626831587786813E-4	0.0	0.0	5.892136890401001	0.0
20	3.8626831587786813E-4	0.0	0.0	6.0383008211291855	0.0
21	3.8626831587786813E-4	0.0	0.0	6.227726803235693	0.0
22	3.8626831587786813E-4	0.0	0.0	6.430672176397924	0.0
23	3.8626831587786813E-4	0.0	0.0	6.650227087142905	0.0
24	3.8626831587786813E-4	0.0	0.0	6.82605642453051	0.0
25	3.8626831587786813E-4	0.0	0.0	6.972761130900924	0.0
26	3.8626831587786813E-4	0.0	0.0	7.113903573522697	0.0
27	3.8626831587786813E-4	0.0	0.0	7.264161948399188	0.0
28	3.8626831587786813E-4	0.0	0.0	7.415424620896961	0.0
29	3.8626831587786813E-4	0.0	0.0	7.586618738494033	0.0
30	3.8626831587786813E-4	0.0	0.0	7.811349644671776	0.0
31	3.8626831587786813E-4	0.0	0.0	7.987951518691138	0.0
32	3.8626831587786813E-4	0.0	0.0	8.16663924161624	0.0
33	3.8626831587786813E-4	0.0	0.0	8.34779908176296	0.0
34	3.8626831587786813E-4	0.0	0.0	8.529267936562382	0.0
35	3.8626831587786813E-4	0.0	0.0	8.734067397640828	0.0
36	3.8626831587786813E-4	0.0	0.0	8.907656378796341	0.0
37	3.8626831587786813E-4	0.0	0.0	9.101176805051153	0.0
38	3.8626831587786813E-4	0.0	0.0	9.320422701143432	0.0
39	3.8626831587786813E-4	0.0	0.0	9.617308528727161	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAAC	20	7.033616E-4	45.000004	34
TCTAACG	20	7.033616E-4	45.000004	1
CCGGTAT	25	3.8910795E-5	45.0	42
CGTTAGG	195	0.0	41.538464	2
TCACGAC	110	0.0	40.909092	25
CGTTTTT	3520	0.0	40.205967	1
GTATGCG	90	0.0	40.0	1
ATAGTCG	45	1.9286745E-8	40.0	10
AGGGCGA	1420	0.0	39.454227	6
ATACGAC	35	6.2489E-6	38.571426	37
TACGGGA	370	0.0	38.31081	4
GGGCGAT	2720	0.0	37.72059	7
TTAGGGA	1345	0.0	37.63941	4
CTCACGA	120	0.0	37.499996	24
CGGTCTA	120	0.0	37.499996	31
TAGGGCG	565	0.0	37.433628	5
GACACGA	750	0.0	37.2	25
CACGACC	745	0.0	37.14765	27
ATGACGG	85	0.0	37.058823	2
CGTATGG	195	0.0	36.92308	2
>>END_MODULE