FastQCFastQC Report
Thu 26 May 2016
SRR1547125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547125_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences721534
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.4756532609689911No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9380.13000080384292356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG351.2110831E-745.0000041
TTAGCCG207.031539E-445.035
GCCGACC207.031539E-445.014
CCGAATT207.031539E-445.033
TTCGAAT207.031539E-445.013
CCCGAAT207.031539E-445.032
TACGTAG302.1643737E-644.9999961
GCGATTC1250.039.69
TAATACG403.4558434E-739.3751
ATTAGCG403.4558434E-739.3751
CGTTAGG800.039.3752
TTACGGG1550.039.193553
TTTACGG750.039.02
TACGAAT750.039.012
CGAATAT750.039.014
CGGAATT356.2450563E-638.5714318
CTATGCG356.2450563E-638.571431
GCGATGA1400.038.571439
ATTGGGA3850.038.571434
CGTTTTT24250.038.4123731
Produced by FastQC (version 0.11.5)