##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547125_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	721534
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529961166071175	31.0	31.0	33.0	30.0	34.0
2	31.908102736669374	31.0	31.0	34.0	30.0	34.0
3	32.02144736076193	33.0	31.0	34.0	30.0	34.0
4	35.75784924896124	37.0	35.0	37.0	35.0	37.0
5	35.69161397799688	37.0	35.0	37.0	33.0	37.0
6	35.7351060379691	37.0	35.0	37.0	33.0	37.0
7	36.05706730382768	37.0	35.0	37.0	35.0	37.0
8	34.72501365147034	37.0	35.0	37.0	33.0	37.0
9	37.06110176374225	39.0	37.0	39.0	34.0	39.0
10	37.00238658192129	39.0	37.0	39.0	32.0	39.0
11	36.921449301072435	39.0	37.0	39.0	33.0	39.0
12	36.50947010120105	39.0	35.0	39.0	32.0	39.0
13	36.35216358480682	39.0	35.0	39.0	32.0	39.0
14	37.427428229300354	40.0	35.0	41.0	32.0	41.0
15	37.61389206884222	40.0	35.0	41.0	33.0	41.0
16	37.69079211790435	40.0	35.0	41.0	33.0	41.0
17	37.60426535686468	39.0	35.0	41.0	33.0	41.0
18	37.53989832772953	39.0	36.0	41.0	33.0	41.0
19	37.49012243359287	39.0	35.0	41.0	33.0	41.0
20	37.3671330803538	39.0	35.0	41.0	33.0	41.0
21	37.24275640510357	39.0	35.0	41.0	32.0	41.0
22	37.16635945083669	39.0	35.0	41.0	32.0	41.0
23	37.10992413385925	39.0	35.0	41.0	32.0	41.0
24	36.95644002916009	39.0	35.0	41.0	32.0	41.0
25	36.85929283997705	38.0	35.0	41.0	32.0	41.0
26	36.75081978118841	38.0	35.0	41.0	31.0	41.0
27	36.64258510340469	38.0	35.0	41.0	31.0	41.0
28	36.687686235160086	38.0	35.0	41.0	31.0	41.0
29	36.65731483201069	38.0	35.0	41.0	31.0	41.0
30	36.601580798687245	38.0	35.0	41.0	31.0	41.0
31	36.4808366618898	38.0	35.0	41.0	31.0	41.0
32	36.404978836756136	38.0	35.0	41.0	31.0	41.0
33	36.27236277153953	38.0	35.0	41.0	30.0	41.0
34	36.11644496309252	38.0	35.0	41.0	30.0	41.0
35	36.01565553390415	38.0	35.0	41.0	30.0	41.0
36	35.88013454667417	38.0	35.0	41.0	30.0	41.0
37	35.835451967613444	38.0	35.0	41.0	29.0	41.0
38	35.75241637954691	38.0	35.0	41.0	29.0	41.0
39	35.67202654344771	38.0	35.0	40.0	29.0	41.0
40	35.52357061482896	38.0	35.0	40.0	28.0	41.0
41	35.430879210127316	38.0	35.0	40.0	28.0	41.0
42	35.37498856602738	38.0	35.0	40.0	28.0	41.0
43	35.29395842746149	37.0	34.0	40.0	27.0	41.0
44	35.163185657224744	37.0	34.0	40.0	27.0	41.0
45	35.0364071547564	37.0	34.0	40.0	27.0	41.0
46	34.97575443430247	37.0	34.0	40.0	27.0	41.0
47	34.904632352737366	37.0	34.0	40.0	27.0	41.0
48	34.847846393932926	36.0	34.0	40.0	26.0	41.0
49	34.783112091737884	36.0	34.0	40.0	26.0	41.0
50	34.67849886491835	36.0	34.0	40.0	26.0	41.0
51	34.31917553434766	35.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	3.0
13	7.0
14	6.0
15	19.0
16	43.0
17	113.0
18	299.0
19	509.0
20	938.0
21	1589.0
22	2243.0
23	3410.0
24	4900.0
25	6897.0
26	9166.0
27	10817.0
28	12067.0
29	13574.0
30	15967.0
31	19689.0
32	24284.0
33	31963.0
34	55585.0
35	88454.0
36	55750.0
37	72886.0
38	105694.0
39	184522.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.03470938306442	24.194701843572165	28.20740256176424	13.563186211599177
2	32.244218567662784	26.168413408099966	27.802847821447084	13.784520202790166
3	29.90531284735023	25.84285702406262	30.429889651769702	13.82194047681745
4	26.55605972830109	28.187860863105552	29.59458597931629	15.661493429277067
5	23.47152039959309	31.862254585369502	29.36410481002974	15.302120205007663
6	21.458032469710368	40.432190305654345	27.365584989757934	10.744192234877358
7	87.09790529621611	4.1518487001305555	6.966546275019611	1.7836997286337166
8	84.81568436137452	6.233108904084908	6.372395479630898	2.578811254909678
9	82.4823223853623	5.37424431835506	8.77865769319256	3.364775603090083
10	45.44900171024512	26.15649435785424	15.503773903932455	12.89073002796819
11	38.03798573594592	24.333988419118157	21.87007126483298	15.757954580102949
12	33.09629206662471	22.635800946317154	26.89797015802443	17.369936829033698
13	23.524463157661316	31.76995124276888	28.027785246433297	16.67780035313651
14	19.749034695523704	33.15366982013322	29.422591312398303	17.674704171944775
15	18.48381365257909	23.64628693866124	40.016548076736505	17.853351332023163
16	21.358938040341826	21.435857492509015	37.927388037154174	19.27781642999498
17	21.640144469976466	22.19424171279524	28.183564461272788	27.982049355955507
18	24.049317149295806	22.947913750426174	31.99558163579263	21.007187464485387
19	28.309130269675443	25.138247123489677	25.76413585499782	20.788486751837056
20	30.11029279285522	23.89353793445631	25.363184548475886	20.63298472421258
21	25.224036566537407	27.038088295215474	28.046218196231916	19.691656942015207
22	25.414325589646502	23.888687158193516	26.668320550382933	24.028666701777045
23	22.98685855413605	27.823636862573352	26.27693220277908	22.91257238051152
24	22.442601457450376	24.352282775309273	33.15547153703083	20.049644230209527
25	21.852608470286917	25.481266302073085	30.58012512230886	22.08600010533114
26	21.106697674676454	29.72126053657901	27.029911272372477	22.142130516372063
27	20.553847774325256	29.141523476371177	27.813103748402707	22.491525000900857
28	19.54599506052383	27.23198075211979	32.42771650400397	20.794307683352415
29	20.552184651035155	25.365956420626055	31.793234968830298	22.28862395950849
30	22.067705749140025	27.08008215829053	30.30598696665715	20.546225125912294
31	27.032405957307624	26.3839264677756	25.671278137967164	20.91238943694961
32	28.390900498105427	25.972857827905543	26.093295672830386	19.542946001158644
33	27.20273750093551	26.69479192941705	25.079344840298585	21.02312572934886
34	22.697336508050903	27.01050816732129	27.80409516391466	22.488060160713147
35	22.475032361607354	27.138014286229062	28.903419658671663	21.48353369349192
36	28.367062397613974	26.600132495488776	25.16457990891628	19.86822519798097
37	23.361892856053906	29.433401613783968	26.39501395637628	20.80969157378585
38	23.7785052402243	29.893532390712014	25.107756529837815	21.220205839225873
39	22.85464025257299	28.170120881344467	26.57851189271746	22.39672697336508
40	25.60669351686823	25.099718100602324	26.426197518065674	22.86739086446377
41	20.29925131733224	25.229025936407712	27.90499131018081	26.566731436079245
42	23.774624619214062	26.251430978997526	25.994894211499386	23.979050190289023
43	22.86004540326582	25.28432478580358	27.707911200303798	24.147718610626804
44	22.833435430624196	27.725928369279895	27.61366754719805	21.826968652897854
45	21.05888288008604	29.681622764831594	25.286680877131225	23.972813477951142
46	22.914789878231655	28.612373082903925	26.81952617617465	21.65331086268977
47	22.43497880903741	26.570612057089477	28.253415639457046	22.740993494416063
48	23.661532235487172	25.000900858448805	29.015126106323475	22.322440799740555
49	23.0090335313374	24.718724273561605	29.869417103005542	22.40282509209545
50	21.122774533147435	29.07513713837463	27.78898846069624	22.013099867781698
51	20.91044912644449	29.29189754051784	26.822575235539837	22.975078097497832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	619.0
1	936.5
2	1254.0
3	3759.5
4	6265.0
5	4475.5
6	2686.0
7	2800.5
8	2915.0
9	3163.5
10	3412.0
11	3481.5
12	3551.0
13	3529.0
14	3507.0
15	3602.0
16	3697.0
17	3507.0
18	3317.0
19	3068.0
20	2819.0
21	3066.0
22	3313.0
23	3391.0
24	3469.0
25	3664.0
26	4868.5
27	5878.0
28	6485.0
29	7092.0
30	8580.5
31	10069.0
32	11306.0
33	12543.0
34	15777.5
35	19012.0
36	20671.0
37	22330.0
38	23959.5
39	25589.0
40	31692.0
41	37795.0
42	44253.5
43	50712.0
44	52431.5
45	54151.0
46	56358.0
47	58565.0
48	57653.5
49	56742.0
50	55052.5
51	53363.0
52	50048.5
53	46734.0
54	43615.5
55	40497.0
56	37816.0
57	35135.0
58	33614.5
59	32094.0
60	30201.5
61	28309.0
62	25464.5
63	22620.0
64	19901.5
65	17183.0
66	15069.5
67	12956.0
68	11055.5
69	9155.0
70	8019.0
71	6883.0
72	5721.0
73	4559.0
74	3927.0
75	2564.5
76	1834.0
77	1298.0
78	762.0
79	690.5
80	619.0
81	435.5
82	252.0
83	153.0
84	54.0
85	42.5
86	31.0
87	28.0
88	25.0
89	16.5
90	8.0
91	5.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	721534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.14665097762608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5064023285867	20.497459505903564
2	6.802727537422395	3.693425403085831
3	2.7535401152699976	2.242481773863807
4	1.631637090730584	1.7717393049684949
5	1.1448154119766596	1.5538952211368795
6	0.9176152705653042	1.4946108889065743
7	0.7121979204676746	1.353365186174894
8	0.6571752632167263	1.4272086001339213
9	0.5898238960041015	1.4410569098778268
>10	8.807255247145482	55.3827655550458
>50	0.4514971493705347	7.397314275095969
>100	0.023763007861591235	1.047984408761498
>500	0.0010331742548517929	0.2154026564776744
>1k	5.165871274258964E-4	0.48129031056730376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3432	0.4756532609689911	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	938	0.13000080384292356	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3859360750844728E-4	0.0	0.0	0.029936219221824614	0.0
2	1.3859360750844728E-4	0.0	0.0	0.1245956531500941	0.0
3	1.3859360750844728E-4	0.0	0.0	0.20331682221489217	0.0
4	1.3859360750844728E-4	0.0	0.0	0.40108990012944645	0.0
5	1.3859360750844728E-4	0.0	0.0	0.6651107224330385	0.0
6	1.3859360750844728E-4	0.0	0.0	1.1561478738354671	0.0
7	1.3859360750844728E-4	0.0	0.0	1.3651470339582057	0.0
8	1.3859360750844728E-4	0.0	0.0	1.8679646419988525	0.0
9	1.3859360750844728E-4	0.0	0.0	2.149586852456017	0.0
10	1.3859360750844728E-4	0.0	0.0	2.599461702428437	0.0
11	1.3859360750844728E-4	0.0	0.0	2.978653812571549	0.0
12	1.3859360750844728E-4	0.0	0.0	3.3025470733187903	0.0
13	1.3859360750844728E-4	0.0	0.0	3.459850817840878	0.0
14	1.3859360750844728E-4	0.0	0.0	3.529009027987593	0.0
15	1.3859360750844728E-4	0.0	0.0	3.6017706719295277	0.0
16	1.3859360750844728E-4	0.0	0.0	3.742443183550602	0.0
17	1.3859360750844728E-4	0.0	0.0	3.8901839691546067	0.0
18	1.3859360750844728E-4	0.0	0.0	4.104726873577683	0.0
19	1.3859360750844728E-4	0.0	0.0	4.207979111171476	0.0
20	1.3859360750844728E-4	0.0	0.0	4.326753832806216	0.0
21	1.3859360750844728E-4	0.0	0.0	4.464100097847087	0.0
22	1.3859360750844728E-4	0.0	0.0	4.600476207635399	0.0
23	1.3859360750844728E-4	0.0	0.0	4.766927130253044	0.0
24	1.3859360750844728E-4	0.0	0.0	4.891245596188122	0.0
25	1.3859360750844728E-4	0.0	0.0	4.997408299539592	0.0
26	1.3859360750844728E-4	0.0	0.0	5.102462254030995	0.0
27	1.3859360750844728E-4	0.0	0.0	5.212782765607719	0.0
28	1.3859360750844728E-4	0.0	0.0	5.320885779464308	0.0
29	1.3859360750844728E-4	0.0	0.0	5.439106126669014	0.0
30	1.3859360750844728E-4	0.0	0.0	5.611793761624539	0.0
31	1.3859360750844728E-4	0.0	0.0	5.750387369132986	0.0
32	1.3859360750844728E-4	0.0	0.0	5.883575825948604	0.0
33	1.3859360750844728E-4	0.0	0.0	6.011913506501426	0.0
34	1.3859360750844728E-4	0.0	0.0	6.1421914975593666	0.0
35	1.3859360750844728E-4	0.0	0.0	6.304484611951758	0.0
36	1.3859360750844728E-4	0.0	0.0	6.438643224019935	0.0
37	1.3859360750844728E-4	0.0	0.0	6.574049178555688	0.0
38	1.3859360750844728E-4	0.0	0.0	6.733016046367878	0.0
39	1.3859360750844728E-4	0.0	0.0	6.9759706403301855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	35	1.2110831E-7	45.000004	1
TTAGCCG	20	7.031539E-4	45.0	35
GCCGACC	20	7.031539E-4	45.0	14
CCGAATT	20	7.031539E-4	45.0	33
TTCGAAT	20	7.031539E-4	45.0	13
CCCGAAT	20	7.031539E-4	45.0	32
TACGTAG	30	2.1643737E-6	44.999996	1
GCGATTC	125	0.0	39.6	9
TAATACG	40	3.4558434E-7	39.375	1
ATTAGCG	40	3.4558434E-7	39.375	1
CGTTAGG	80	0.0	39.375	2
TTACGGG	155	0.0	39.19355	3
TTTACGG	75	0.0	39.0	2
TACGAAT	75	0.0	39.0	12
CGAATAT	75	0.0	39.0	14
CGGAATT	35	6.2450563E-6	38.57143	18
CTATGCG	35	6.2450563E-6	38.57143	1
GCGATGA	140	0.0	38.57143	9
ATTGGGA	385	0.0	38.57143	4
CGTTTTT	2425	0.0	38.412373	1
>>END_MODULE