FastQCFastQC Report
Thu 26 May 2016
SRR1547124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547124_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1295719
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75810.5850805614488944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT302.165716E-645.00000444
ATACCGG207.0336193E-445.02
TATACGG1800.041.2500042
CGTTTTT47150.041.0869561
TAACGCC501.0822987E-940.49999612
CGTAAGG2550.038.823532
CGTATGG1450.038.7931022
GCGCGAC2650.038.2075469
TCGATAG659.094947E-1238.0769271
TCGCAAG950.037.8947371
CACGACC7350.037.6530627
ACGGGAT4250.037.5882345
AACACGT6350.037.55905541
GGGCGAT30100.037.5249187
GTCGTTG301.14000206E-437.5000041
CGTCGAA301.14000206E-437.50000431
GACACGA7300.037.2945225
GGACCGA9850.037.23358
AGGGCGA15100.037.102656
TAAGGGA14750.037.0677954
Produced by FastQC (version 0.11.5)