##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547124_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1295719
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45584575050609	31.0	31.0	33.0	30.0	34.0
2	31.82031443546016	31.0	31.0	34.0	30.0	34.0
3	31.93234412708311	33.0	31.0	34.0	30.0	34.0
4	35.67683116478187	37.0	35.0	37.0	33.0	37.0
5	35.6136708653651	37.0	35.0	37.0	33.0	37.0
6	35.6654984607002	37.0	35.0	37.0	33.0	37.0
7	36.03713922540304	37.0	35.0	37.0	35.0	37.0
8	34.723299573441466	37.0	35.0	37.0	33.0	37.0
9	37.02741643828639	39.0	37.0	39.0	34.0	39.0
10	36.900920647146485	39.0	37.0	39.0	32.0	39.0
11	36.823689395617414	39.0	37.0	39.0	32.0	39.0
12	36.5703474287249	39.0	35.0	39.0	32.0	39.0
13	36.51345700726778	39.0	35.0	39.0	32.0	39.0
14	37.610092929099594	40.0	36.0	41.0	33.0	41.0
15	37.75558512300893	40.0	36.0	41.0	33.0	41.0
16	37.782715233781396	40.0	36.0	41.0	33.0	41.0
17	37.67083063534609	40.0	36.0	41.0	33.0	41.0
18	37.60908190741974	39.0	36.0	41.0	33.0	41.0
19	37.560747353399925	39.0	36.0	41.0	32.0	41.0
20	37.458584770308995	39.0	35.0	41.0	32.0	41.0
21	37.360509493184864	39.0	35.0	41.0	32.0	41.0
22	37.28434020030578	39.0	35.0	41.0	32.0	41.0
23	37.203476988451975	39.0	35.0	41.0	32.0	41.0
24	37.063817849394816	39.0	35.0	41.0	32.0	41.0
25	36.974786971557876	39.0	35.0	41.0	31.0	41.0
26	36.86227337871869	39.0	35.0	41.0	31.0	41.0
27	36.75152714438856	39.0	35.0	41.0	31.0	41.0
28	36.771837103569524	39.0	35.0	41.0	31.0	41.0
29	36.744034007373514	39.0	35.0	41.0	31.0	41.0
30	36.670351364763505	38.0	35.0	41.0	31.0	41.0
31	36.57259714490565	38.0	35.0	41.0	31.0	41.0
32	36.51635732747609	39.0	35.0	41.0	30.0	41.0
33	36.37068531062676	39.0	35.0	41.0	30.0	41.0
34	36.24570296491755	39.0	35.0	41.0	30.0	41.0
35	36.11156662825813	39.0	35.0	41.0	30.0	41.0
36	36.01889993123509	38.0	35.0	41.0	30.0	41.0
37	35.99475889448252	38.0	35.0	41.0	29.0	41.0
38	35.89224361146206	38.0	35.0	41.0	29.0	41.0
39	35.81845600782268	38.0	35.0	41.0	29.0	41.0
40	35.70263305546959	38.0	35.0	40.0	28.0	41.0
41	35.62611029088869	38.0	35.0	40.0	28.0	41.0
42	35.56654027609381	38.0	35.0	40.0	28.0	41.0
43	35.48062735824666	38.0	35.0	40.0	28.0	41.0
44	35.35961886797986	38.0	34.0	40.0	27.0	41.0
45	35.2302026905525	38.0	34.0	40.0	27.0	41.0
46	35.154729536265194	37.0	34.0	40.0	27.0	41.0
47	35.07763951906239	37.0	34.0	40.0	26.0	41.0
48	34.99263806427165	37.0	34.0	40.0	26.0	41.0
49	34.923136112073685	37.0	34.0	40.0	26.0	41.0
50	34.81935280720588	37.0	34.0	40.0	26.0	41.0
51	34.46459533278435	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	1.0
13	6.0
14	15.0
15	50.0
16	97.0
17	191.0
18	470.0
19	905.0
20	1646.0
21	2540.0
22	3944.0
23	5625.0
24	8555.0
25	12380.0
26	16314.0
27	19725.0
28	22242.0
29	24811.0
30	29449.0
31	35788.0
32	44456.0
33	56906.0
34	91820.0
35	131519.0
36	104871.0
37	137256.0
38	198363.0
39	345533.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.7633244553796	23.82044254965776	27.569017665095597	13.847215329867046
2	32.421072778897276	25.255475917232058	28.380381857486075	13.943069446384596
3	29.753596265857023	25.683809529689693	30.889799408668082	13.672794795785197
4	25.54597100142855	28.245707595551195	30.759678603153922	15.448642799866331
5	23.188592588362138	32.56979329623167	29.153311790596568	15.088302324809625
6	21.86361394715984	40.852067462158075	26.57165635450279	10.712662236179295
7	88.16695595264096	3.457462613421583	6.660240376192678	1.7153410577447732
8	85.56600620967973	5.729328658451408	6.297738938766815	2.406926193102054
9	82.62100038665791	5.203906093836704	8.704819486323808	3.4702740331815773
10	41.25647613410006	30.866569063199663	15.19851140563656	12.678443397063715
11	32.228438419132544	25.985418134641847	24.938354689558462	16.84778875666715
12	28.80701757093938	23.169915699314434	28.927722754702216	19.09534397504397
13	23.573166712844372	28.073988264430795	30.30742005018063	18.045424972544204
14	20.772868191328524	29.68197579876501	30.83701018507871	18.70814582482776
15	20.204535088240583	24.458003625786144	36.70062721932765	18.636834066645623
16	23.257743384175118	22.825628087571456	34.592762782671244	19.323865745582182
17	23.055770579886534	23.204799806130804	29.106002150157558	24.633427463825104
18	23.987685601584914	23.5738613078916	31.305784664730545	21.13266842579294
19	26.991654826393685	25.733511664180277	27.002305283784523	20.272528225641516
20	28.12384475337631	25.459841215572204	26.374391361089867	20.04192266996162
21	25.854062493488172	26.579528431704713	28.41248758411353	19.153921490693584
22	25.516180591625194	23.622791670107485	27.853801634459323	23.007226103808
23	23.089265496608448	27.350760465810875	27.41798183093711	22.14199220664357
24	22.55620238647423	25.3666883020161	32.0783287117037	19.998780599805976
25	23.109948993570363	26.28849310691593	29.109397948166233	21.492159951347475
26	21.637407493445725	28.136887704818715	27.627363649062797	22.598341152672763
27	21.468235010831823	27.002536815466932	28.835650322330693	22.693577851370552
28	20.46076348344047	27.475247333719732	31.281165129167665	20.78282405367213
29	22.17872856691922	25.016303689303005	30.6586536123959	22.14631413138188
30	23.677819033293485	26.48259383400259	29.523145064632068	20.316442068071858
31	25.850126454887207	26.381954729381913	26.794621364663172	20.973297451067708
32	26.516628991316786	25.685893314831382	27.064818838035098	20.732658855816734
33	25.016149334848066	25.83075497079228	27.100551894353636	22.05254380000602
34	22.35145120199673	27.38657069935688	29.043179886997105	21.218798211649283
35	21.804959254282757	27.176339931729025	29.360609823580575	21.65809099040764
36	25.42071236124499	27.53475097609898	26.655239291852634	20.38929737080339
37	22.761339457089075	28.62341294678862	27.188842642579143	21.42640495354317
38	22.8298728350823	29.17839438952427	26.64057561863336	21.35115715676007
39	22.656532782185028	27.300132204590653	26.614566892976022	23.428768120248293
40	23.690244566916128	26.071933806635545	27.485743436655635	22.752078189792694
41	21.22026457897121	25.952309104057285	27.76296403772732	25.064462279244186
42	23.264612157419933	26.985712179878508	26.246740226854744	23.502935435846815
43	22.283380887368327	26.258625519885097	27.963624829148912	23.494368763597663
44	22.479488222369202	26.692978956085383	28.046358816996587	22.781174004548827
45	21.552126657091545	27.686249873622288	27.355468276686533	23.40615519259963
46	23.265692638604513	27.343351451973767	27.463593572371785	21.92736233704993
47	22.339488731738903	26.70741109762225	28.246710899508304	22.706389271130544
48	22.408176464187065	26.204369928973797	29.18040099743849	22.207052609400648
49	22.87710529829384	25.49040339765026	28.964073228840515	22.66841807521538
50	21.710648682314606	27.115832985392668	28.877866265756698	22.295652066536032
51	21.42717672581787	27.508279187076827	27.734254109108537	23.330289977996774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1041.0
1	1553.5
2	2066.0
3	7574.0
4	13082.0
5	9020.5
6	4959.0
7	4968.0
8	4977.0
9	5265.0
10	5553.0
11	5796.5
12	6040.0
13	6051.5
14	6063.0
15	6075.0
16	6087.0
17	5887.0
18	5687.0
19	5743.0
20	5799.0
21	5776.5
22	5754.0
23	6241.0
24	6728.0
25	8103.0
26	10739.5
27	12001.0
28	14288.0
29	16575.0
30	18884.5
31	21194.0
32	24183.5
33	27173.0
34	31706.5
35	36240.0
36	38971.5
37	41703.0
38	46792.0
39	51881.0
40	58813.0
41	65745.0
42	73416.5
43	81088.0
44	85564.0
45	90040.0
46	93595.0
47	97150.0
48	99974.0
49	102798.0
50	100626.0
51	98454.0
52	91803.5
53	85153.0
54	79327.0
55	73501.0
56	68191.0
57	62881.0
58	59515.0
59	56149.0
60	52589.5
61	49030.0
62	45409.0
63	41788.0
64	37191.5
65	32595.0
66	28004.0
67	23413.0
68	19578.5
69	15744.0
70	13028.0
71	10312.0
72	9264.5
73	8217.0
74	6588.5
75	4254.5
76	3549.0
77	2684.0
78	1819.0
79	1273.0
80	727.0
81	496.5
82	266.0
83	192.0
84	118.0
85	106.5
86	95.0
87	64.0
88	33.0
89	20.5
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1295719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.877693228789333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.33432338644035	20.24812833579154
2	6.3776419335474825	3.4283260682590426
3	2.734344022045555	2.2047857941954323
4	1.70413148731615	1.8321249335041596
5	1.240459828126123	1.667034936150534
6	1.0194467307979629	1.64402258960879
7	0.8713312544068442	1.6393561909622352
8	0.7400988799406031	1.5913720523211297
9	0.6802179237787781	1.6454419815645005
>10	8.763634364159161	53.65836961824937
>50	0.5021370784956394	8.10427245085399
>100	0.030458811260766494	1.479235861259391
>500	0.0014785830704875593	0.25497762839872407
>1k	0.0	0.0
>5k	2.9571661409751184E-4	0.6025515588811493
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7581	0.5850805614488944	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043142070155643315	0.0
2	0.0	0.0	0.0	0.15057277079366746	0.0
3	0.0	0.0	0.0	0.241873430890494	0.0
4	0.0	0.0	0.0	0.4242432194017376	0.0
5	0.0	0.0	0.0	0.7299422174097934	0.0
6	0.0	0.0	0.0	1.3365552253227744	0.0
7	0.0	0.0	0.0	1.6203358907293943	0.0
8	0.0	0.0	0.0	2.297180175639934	0.0
9	0.0	0.0	0.0	2.6478734972629097	0.0
10	0.0	0.0	0.0	3.134938979825101	0.0
11	0.0	0.0	0.0	3.5324017012948024	0.0
12	0.0	0.0	0.0	3.8623343487283894	0.0
13	0.0	0.0	0.0	4.042234465960598	0.0
14	0.0	0.0	0.0	4.1309882775509195	0.0
15	0.0	0.0	0.0	4.215728873312809	0.0
16	0.0	0.0	0.0	4.372861708441413	0.0
17	0.0	0.0	0.0	4.53369905048857	0.0
18	0.0	0.0	0.0	4.757513010151121	0.0
19	0.0	0.0	0.0	4.878218193913958	0.0
20	7.717722746984493E-5	0.0	0.0	5.001470226183301	0.0
21	7.717722746984493E-5	0.0	0.0	5.155052908848292	0.0
22	7.717722746984493E-5	0.0	0.0	5.328933202337852	0.0
23	7.717722746984493E-5	0.0	0.0	5.514698788857769	0.0
24	7.717722746984493E-5	0.0	0.0	5.676539434862034	0.0
25	7.717722746984493E-5	0.0	0.0	5.815458444307755	0.0
26	7.717722746984493E-5	0.0	0.0	5.9485119844657675	0.0
27	7.717722746984493E-5	0.0	0.0	6.087662525593898	0.0
28	7.717722746984493E-5	0.0	0.0	6.23128934591528	0.0
29	7.717722746984493E-5	0.0	0.0	6.381553407799068	0.0
30	7.717722746984493E-5	0.0	0.0	6.580516300216328	0.0
31	7.717722746984493E-5	0.0	0.0	6.745366858091916	0.0
32	7.717722746984493E-5	0.0	0.0	6.926115924826293	0.0
33	7.717722746984493E-5	0.0	0.0	7.096600420307181	0.0
34	7.717722746984493E-5	0.0	0.0	7.261759687092649	0.0
35	7.717722746984493E-5	0.0	0.0	7.454934287449671	0.0
36	7.717722746984493E-5	0.0	0.0	7.628197163119473	0.0
37	7.717722746984493E-5	0.0	0.0	7.804084064523249	0.0
38	7.717722746984493E-5	0.0	0.0	7.9977989054725604	0.0
39	7.717722746984493E-5	0.0	0.0	8.27123782239822	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	30	2.165716E-6	45.000004	44
ATACCGG	20	7.0336193E-4	45.0	2
TATACGG	180	0.0	41.250004	2
CGTTTTT	4715	0.0	41.086956	1
TAACGCC	50	1.0822987E-9	40.499996	12
CGTAAGG	255	0.0	38.82353	2
CGTATGG	145	0.0	38.793102	2
GCGCGAC	265	0.0	38.207546	9
TCGATAG	65	9.094947E-12	38.076927	1
TCGCAAG	95	0.0	37.894737	1
CACGACC	735	0.0	37.65306	27
ACGGGAT	425	0.0	37.588234	5
AACACGT	635	0.0	37.559055	41
GGGCGAT	3010	0.0	37.524918	7
GTCGTTG	30	1.14000206E-4	37.500004	1
CGTCGAA	30	1.14000206E-4	37.500004	31
GACACGA	730	0.0	37.29452	25
GGACCGA	985	0.0	37.2335	8
AGGGCGA	1510	0.0	37.10265	6
TAAGGGA	1475	0.0	37.067795	4
>>END_MODULE