##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547123_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	880229
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41967374399162	31.0	31.0	33.0	30.0	34.0
2	31.777782826968892	31.0	31.0	34.0	30.0	34.0
3	31.894354764498782	31.0	31.0	34.0	30.0	34.0
4	35.64055944532616	37.0	35.0	37.0	33.0	37.0
5	35.57277594807715	37.0	35.0	37.0	33.0	37.0
6	35.62142124378997	37.0	35.0	37.0	33.0	37.0
7	35.996408889050464	37.0	35.0	37.0	35.0	37.0
8	34.69051462744354	37.0	35.0	37.0	33.0	37.0
9	37.00789226439938	39.0	37.0	39.0	34.0	39.0
10	36.83332178330866	39.0	37.0	39.0	32.0	39.0
11	36.741950106165554	39.0	35.0	39.0	32.0	39.0
12	36.29825193216765	38.0	35.0	39.0	32.0	39.0
13	36.13640882088638	38.0	35.0	39.0	32.0	39.0
14	37.16591591506301	39.0	35.0	41.0	32.0	41.0
15	37.386338100653354	39.0	35.0	41.0	32.0	41.0
16	37.45485549783068	39.0	35.0	41.0	33.0	41.0
17	37.386146105161274	39.0	35.0	41.0	32.0	41.0
18	37.324621206526935	39.0	35.0	41.0	32.0	41.0
19	37.27444449114946	39.0	35.0	41.0	32.0	41.0
20	37.1259376821259	39.0	35.0	41.0	32.0	41.0
21	37.02468562158256	39.0	35.0	41.0	32.0	41.0
22	36.94724668239742	39.0	35.0	41.0	32.0	41.0
23	36.88346782485012	38.0	35.0	41.0	32.0	41.0
24	36.74069929529702	38.0	35.0	41.0	31.0	41.0
25	36.63452578817558	38.0	35.0	41.0	31.0	41.0
26	36.51066938262657	38.0	35.0	40.0	31.0	41.0
27	36.40325756138459	38.0	35.0	40.0	31.0	41.0
28	36.43990938721628	38.0	35.0	40.0	31.0	41.0
29	36.419405631943505	38.0	35.0	40.0	31.0	41.0
30	36.356927572256765	38.0	35.0	40.0	31.0	41.0
31	36.25897124498284	38.0	35.0	40.0	30.0	41.0
32	36.180098587981085	38.0	35.0	40.0	30.0	41.0
33	36.05216142617432	38.0	35.0	41.0	30.0	41.0
34	35.91306694053479	38.0	35.0	41.0	30.0	41.0
35	35.8193913174867	38.0	35.0	41.0	30.0	41.0
36	35.69751735059854	38.0	35.0	40.0	29.0	41.0
37	35.64257369389102	38.0	35.0	40.0	29.0	41.0
38	35.551106587035875	38.0	35.0	40.0	29.0	41.0
39	35.47653849168796	38.0	35.0	40.0	28.0	41.0
40	35.33756102105247	38.0	34.0	40.0	27.0	41.0
41	35.246693758101586	37.0	34.0	40.0	27.0	41.0
42	35.17001712054477	37.0	34.0	40.0	27.0	41.0
43	35.08376229367585	37.0	34.0	40.0	27.0	41.0
44	34.972608264440275	37.0	34.0	40.0	27.0	41.0
45	34.84826902999106	37.0	34.0	40.0	26.0	41.0
46	34.81233065486368	36.0	34.0	40.0	26.0	41.0
47	34.72611899857878	36.0	34.0	40.0	26.0	41.0
48	34.635107454991825	36.0	34.0	40.0	26.0	41.0
49	34.57934014898396	36.0	34.0	40.0	26.0	41.0
50	34.460898243525264	36.0	34.0	40.0	26.0	41.0
51	34.08455413307219	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	2.0
13	7.0
14	10.0
15	33.0
16	82.0
17	184.0
18	415.0
19	759.0
20	1276.0
21	2048.0
22	3069.0
23	4469.0
24	6631.0
25	9136.0
26	11904.0
27	14332.0
28	15518.0
29	17761.0
30	21232.0
31	25779.0
32	32273.0
33	41502.0
34	70032.0
35	110538.0
36	68760.0
37	87562.0
38	125966.0
39	208774.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.29709768707916	24.395356208441214	28.728319562295724	12.579226542183907
2	31.39376230503653	26.111046102775525	28.883847271562285	13.611344320625655
3	29.748849447132507	25.923708489495347	30.69803426153876	13.629407801833388
4	25.27058299601581	29.266929401326248	30.505129915056195	14.95735768760175
5	22.71738377172304	32.345900896244046	30.333810860582872	14.602904471450042
6	21.250038342295017	42.114949632425194	26.22783389322551	10.407178132054272
7	87.75693597915996	3.9381797236855407	6.581128320016723	1.7237559771377677
8	85.48809457538891	5.94493023974443	5.988782464563199	2.5781927203034667
9	83.26821770243879	4.5856248771626476	8.744315399742566	3.4018420206559883
10	45.764795297587334	23.43208415082893	16.45230956944159	14.350810982142148
11	39.140723607152225	23.992279281868694	20.98033579897958	15.886661311999491
12	33.60455063398275	22.374404842376244	25.929161615897684	18.091882907743326
13	23.388231926010164	31.48919201707737	28.13052058043986	16.9920554764726
14	20.009565692564095	33.83335472928067	29.008701144815724	17.148378433339506
15	18.631628814774338	24.090435557110705	40.07400346955167	17.203932158563283
16	20.78163750569454	21.56257064922878	38.61177034612584	19.04402149895084
17	21.57495379043408	21.64845739006554	28.327855592124322	28.448733227376056
18	23.41095328601989	23.65191330892302	31.851370495632388	21.08576290942471
19	28.460548334581116	26.03277101754203	25.644349368175785	19.862331279701078
20	29.338956112557074	24.90306499785851	24.798773955413875	20.95920493417054
21	25.1971930031844	27.15520620202243	27.912395524346508	19.735205270446667
22	25.224004207995876	23.98262270386456	26.48049541653365	24.31287767160591
23	22.94834639622189	28.299567498912214	25.13913992835955	23.61294617650634
24	22.45404320921033	25.51142941211889	32.40713496147025	19.62739241720052
25	22.100271633858917	25.42315692848111	30.623280987106767	21.85329045055321
26	20.092044229399395	29.97515419282937	27.149753075620094	22.783048502151146
27	21.090307181426653	28.9530338127919	27.805605132300798	22.15105387348065
28	19.237380272633597	27.996691769982583	32.6140129443588	20.15191501302502
29	20.71904015886775	25.655710048180648	31.210514536558097	22.414735256393506
30	21.497587559600966	28.360460743738276	29.018016902419713	21.123934794241045
31	26.86244147829713	26.29611158005474	25.2997799436283	21.541666998019835
32	28.37341191894382	26.10207116557169	25.7544343574229	19.770082558061596
33	26.212610582018996	26.753833377450643	24.921355692666342	22.11220034786402
34	21.852267989352768	27.625424747423683	27.957724637565907	22.564582625657643
35	21.513492511607772	27.070001101985962	28.2801407361039	23.136365650302366
36	27.496821849768637	25.96642464631363	26.312470959261734	20.224282544655996
37	22.68421058610884	29.395645905781336	26.873234124301742	21.046909383808078
38	22.863595723385618	29.673528138700274	25.699448666199366	21.763427471714746
39	22.532659114843977	28.186755946463933	26.176938046803727	23.10364689188836
40	24.027724603483865	25.045868745519634	27.406050016529786	23.52035663446671
41	21.049635947009243	24.37343009603183	27.716651007862726	26.860282949096202
42	23.095126381884715	26.789165092265765	25.221164037994658	24.894544487854866
43	22.66137561929907	25.779200639833498	27.04148579517376	24.517937945693678
44	22.264660673529274	27.421273327736305	27.57509693500214	22.738969063732277
45	19.966849535745812	30.374595701800327	25.918823397093256	23.739731365360605
46	22.868026388587516	28.226177506080802	26.608871100588598	22.296925004743084
47	22.906652700604045	26.45129846892116	27.551580327392077	23.09046850308272
48	23.122392013896384	25.354311207651648	28.813638269132234	22.709658509319734
49	22.87825100059189	24.276977922790547	29.421889076592567	23.422882000024995
50	21.748658587708427	28.20425139367142	27.186107251635654	22.8609827669845
51	20.554083085197146	29.23012079811049	26.268164307242774	23.94763180944959
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	848.0
1	1197.0
2	1546.0
3	4454.5
4	7363.0
5	5289.5
6	3216.0
7	3390.0
8	3564.0
9	3799.0
10	4034.0
11	4142.5
12	4251.0
13	4295.5
14	4340.0
15	4316.0
16	4292.0
17	4142.5
18	3993.0
19	3906.0
20	3819.0
21	3973.5
22	4128.0
23	4143.0
24	4158.0
25	4426.5
26	5067.0
27	5439.0
28	6889.0
29	8339.0
30	9670.5
31	11002.0
32	13186.0
33	15370.0
34	17925.5
35	20481.0
36	22972.0
37	25463.0
38	28621.5
39	31780.0
40	39924.0
41	48068.0
42	54520.0
43	60972.0
44	63652.5
45	66333.0
46	70443.5
47	74554.0
48	75612.5
49	76671.0
50	72672.5
51	68674.0
52	62647.5
53	56621.0
54	52049.0
55	47477.0
56	44830.5
57	42184.0
58	40474.5
59	38765.0
60	35473.0
61	32181.0
62	29887.5
63	27594.0
64	24696.0
65	21798.0
66	18683.0
67	15568.0
68	13309.5
69	11051.0
70	9227.0
71	7403.0
72	6340.0
73	5277.0
74	4237.5
75	2472.5
76	1747.0
77	1346.5
78	946.0
79	745.0
80	544.0
81	401.5
82	259.0
83	204.0
84	149.0
85	94.5
86	40.0
87	26.5
88	13.0
89	13.5
90	14.0
91	9.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	880229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.971884023298873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99066230241667	22.30564539102584
2	7.09365480145086	4.11033088417903
3	2.7860329174313296	2.4214986770674045
4	1.7080274325778169	1.9793909074102976
5	1.1859399080940172	1.7179456737950798
6	0.8521521966196113	1.4813072766397655
7	0.7173199396984477	1.4547477070380215
8	0.5882424149238541	1.3633992818207326
9	0.508026211503864	1.3246628832437264
>10	6.830711918569435	46.79172647217808
>50	0.7056653581432227	12.641937063222858
>100	0.03113824204765762	1.5624484225135997
>500	0.0020219637693284173	0.3713978328860312
>1k	4.043927538656834E-4	0.47356152697960185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4042	0.45919868579653705	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	882	0.10020119764288611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1360680004862371E-4	0.0	0.0	0.042829763618331136	0.0
2	1.1360680004862371E-4	0.0	0.0	0.17313676327410255	0.0
3	1.1360680004862371E-4	0.0	0.0	0.2884476653234556	0.0
4	1.1360680004862371E-4	0.0	0.0	0.5306573630271213	0.0
5	1.1360680004862371E-4	0.0	0.0	0.8770444963753751	0.0
6	1.1360680004862371E-4	0.0	0.0	1.6050368710869558	0.0
7	1.1360680004862371E-4	0.0	0.0	1.912002444818337	0.0
8	1.1360680004862371E-4	0.0	0.0	2.707022831558606	0.0
9	1.1360680004862371E-4	0.0	0.0	3.1008976073271843	0.0
10	1.1360680004862371E-4	0.0	0.0	3.7155103955902384	0.0
11	1.1360680004862371E-4	0.0	0.0	4.219356553805885	0.0
12	1.1360680004862371E-4	0.0	0.0	4.6514032143908	0.0
13	1.1360680004862371E-4	0.0	0.0	4.86691531408304	0.0
14	1.1360680004862371E-4	0.0	0.0	4.963253880524273	0.0
15	1.1360680004862371E-4	0.0	0.0	5.060955728566089	0.0
16	1.1360680004862371E-4	0.0	0.0	5.264652721053271	0.0
17	1.1360680004862371E-4	0.0	0.0	5.48516351994765	0.0
18	1.1360680004862371E-4	0.0	0.0	5.805648302884817	0.0
19	1.1360680004862371E-4	0.0	0.0	5.960948798551286	0.0
20	1.1360680004862371E-4	0.0	0.0	6.128632435423055	0.0
21	1.1360680004862371E-4	0.0	0.0	6.359481453121858	0.0
22	1.1360680004862371E-4	0.0	0.0	6.593965888422217	0.0
23	1.1360680004862371E-4	0.0	0.0	6.887980286948055	0.0
24	1.1360680004862371E-4	0.0	0.0	7.101220250639322	0.0
25	1.1360680004862371E-4	0.0	0.0	7.272993732312841	0.0
26	1.1360680004862371E-4	0.0	0.0	7.443517539185826	0.0
27	1.1360680004862371E-4	0.0	0.0	7.616199875259734	0.0
28	1.1360680004862371E-4	0.0	0.0	7.815579809345068	0.0
29	1.1360680004862371E-4	0.0	0.0	8.014505316230208	0.0
30	1.1360680004862371E-4	1.1360680004862371E-4	0.0	8.250580246731248	0.0
31	1.1360680004862371E-4	1.1360680004862371E-4	0.0	8.459048724820473	0.0
32	1.1360680004862371E-4	1.1360680004862371E-4	0.0	8.650021755702209	0.0
33	1.1360680004862371E-4	1.1360680004862371E-4	0.0	8.86303450579338	0.0
34	1.1360680004862371E-4	1.1360680004862371E-4	0.0	9.054234750275212	0.0
35	1.1360680004862371E-4	1.1360680004862371E-4	0.0	9.300647899580678	0.0
36	1.1360680004862371E-4	1.1360680004862371E-4	0.0	9.513206222471652	0.0
37	1.1360680004862371E-4	1.1360680004862371E-4	0.0	9.72633257936287	0.0
38	1.1360680004862371E-4	1.1360680004862371E-4	0.0	9.96081701466323	0.0
39	1.1360680004862371E-4	1.1360680004862371E-4	0.0	10.26289749599252	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	45	3.8380676E-10	45.000004	1
TCACGAA	20	7.032387E-4	45.0	35
ACTTAGC	20	7.032387E-4	45.0	30
TTATCGC	20	7.032387E-4	45.0	45
CGTTATT	25	3.8900584E-5	45.0	32
ACGTATC	20	7.032387E-4	45.0	12
TACACCG	20	7.032387E-4	45.0	29
TACGTAG	25	3.8900584E-5	45.0	1
TCAACGG	30	2.1649212E-6	44.999996	2
TACGGGA	170	0.0	42.35294	4
CGTTTTT	2905	0.0	41.979347	1
CGGGATA	120	0.0	41.249996	6
GGACGTA	50	1.0804797E-9	40.5	8
CGTAAGG	95	0.0	40.263157	2
GCGATAT	135	0.0	40.000004	9
TCGTTAG	40	3.4569894E-7	39.375	1
GCGTAAG	40	3.4569894E-7	39.375	1
GGGCGAT	1850	0.0	38.7973	7
TAATACG	35	6.2466224E-6	38.571426	1
AGGGCGA	940	0.0	38.29787	6
>>END_MODULE