FastQCFastQC Report
Thu 26 May 2016
SRR1547122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547122_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences967176
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59910.6194322439762774No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCT207.032731E-445.036
CGTTTTT34950.040.2360531
CGCAGTA850.039.70588337
GCGATTT1600.039.3759
CATACGA1500.039.018
GCCGATG1700.038.3823559
ACGGGAT3600.038.1255
GGCGATA4550.037.5824178
CACGACC7800.037.527
TAACGCC301.1397882E-437.49999612
GACACGA7850.037.26114725
AACACGT6850.037.11678741
AAGGGAT12550.037.1115535
GGCGATT5400.037.0833368
GCGTATG850.037.0588261
ACACGAC7900.037.0253226
AGGGCGA11500.036.9782646
GCGGGAT4450.036.910115
ACGACCA8000.036.562528
ATTGGGA8700.036.4655154
Produced by FastQC (version 0.11.5)