##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547122_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	967176
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.421585109638784	31.0	31.0	33.0	30.0	34.0
2	31.781215621562158	31.0	31.0	34.0	30.0	34.0
3	31.895640503900015	31.0	31.0	34.0	30.0	34.0
4	35.64727412590883	37.0	35.0	37.0	33.0	37.0
5	35.58300454105561	37.0	35.0	37.0	33.0	37.0
6	35.62779163254671	37.0	35.0	37.0	33.0	37.0
7	36.00291363722838	37.0	35.0	37.0	35.0	37.0
8	34.68572317758092	37.0	35.0	37.0	33.0	37.0
9	36.983633795710396	39.0	37.0	39.0	33.0	39.0
10	36.857235911561084	39.0	37.0	39.0	32.0	39.0
11	36.7682210890262	39.0	37.0	39.0	32.0	39.0
12	36.471559467976874	39.0	35.0	39.0	32.0	39.0
13	36.38486893802162	39.0	35.0	39.0	32.0	39.0
14	37.42796037122509	40.0	36.0	41.0	32.0	41.0
15	37.58358871601446	40.0	36.0	41.0	32.0	41.0
16	37.64255006327701	40.0	35.0	41.0	33.0	41.0
17	37.52086797025567	39.0	35.0	41.0	32.0	41.0
18	37.46882470201908	39.0	36.0	41.0	32.0	41.0
19	37.434558963415135	39.0	36.0	41.0	32.0	41.0
20	37.32079890319859	39.0	35.0	41.0	32.0	41.0
21	37.21492262008156	39.0	35.0	41.0	32.0	41.0
22	37.14487849160856	39.0	35.0	41.0	32.0	41.0
23	37.07095502783361	39.0	35.0	41.0	32.0	41.0
24	36.92249704293738	39.0	35.0	41.0	31.0	41.0
25	36.82300119109655	38.0	35.0	41.0	31.0	41.0
26	36.71821467861072	38.0	35.0	41.0	31.0	41.0
27	36.61544641306236	38.0	35.0	41.0	31.0	41.0
28	36.633430730291074	38.0	35.0	41.0	31.0	41.0
29	36.59185608410465	38.0	35.0	41.0	31.0	41.0
30	36.51827278592521	38.0	35.0	41.0	31.0	41.0
31	36.43059277732284	38.0	35.0	41.0	30.0	41.0
32	36.37540013399836	38.0	35.0	41.0	30.0	41.0
33	36.269303622091535	38.0	35.0	41.0	30.0	41.0
34	36.13085105503031	38.0	35.0	41.0	30.0	41.0
35	36.031835984350316	38.0	35.0	41.0	30.0	41.0
36	35.92274828986658	38.0	35.0	41.0	29.0	41.0
37	35.90402160516803	38.0	35.0	41.0	29.0	41.0
38	35.78692709496514	38.0	35.0	41.0	29.0	41.0
39	35.739182940850476	38.0	35.0	40.0	29.0	41.0
40	35.598953034401184	38.0	35.0	40.0	28.0	41.0
41	35.50635561676469	38.0	35.0	40.0	28.0	41.0
42	35.45334975226846	38.0	34.0	40.0	27.0	41.0
43	35.364691638336765	38.0	34.0	40.0	27.0	41.0
44	35.25726961793924	38.0	34.0	40.0	27.0	41.0
45	35.12089216440441	37.0	34.0	40.0	27.0	41.0
46	35.04449552098067	37.0	34.0	40.0	26.0	41.0
47	34.97847030943696	37.0	34.0	40.0	26.0	41.0
48	34.91287935184496	37.0	34.0	40.0	26.0	41.0
49	34.82547643862131	37.0	34.0	40.0	26.0	41.0
50	34.718849516530604	36.0	34.0	40.0	26.0	41.0
51	34.3681005318577	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	3.0
13	7.0
14	13.0
15	24.0
16	73.0
17	167.0
18	404.0
19	724.0
20	1272.0
21	1974.0
22	3239.0
23	4528.0
24	6782.0
25	9432.0
26	12669.0
27	15168.0
28	16636.0
29	19240.0
30	22879.0
31	27953.0
32	34266.0
33	44110.0
34	71056.0
35	102993.0
36	77571.0
37	100316.0
38	145131.0
39	248380.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.102194429969316	22.93956839292952	27.626926226457233	14.331310950643935
2	32.13117364368016	25.102980222834315	28.714732375493192	14.05111375799234
3	29.868607161468024	25.582727445676902	30.579542916697683	13.96912247615739
4	25.687672150673713	28.754125412541253	29.969312720745762	15.588889716039272
5	22.92943580072293	32.12993291810384	29.662439928203348	15.278191352969884
6	21.82591379438696	40.7948501617079	26.62659123055163	10.752644813353514
7	88.19997601263886	3.455213942446876	6.694645028412616	1.65016501650165
8	85.81592181774569	5.648093004789201	6.047089671373152	2.4888955060919624
9	82.88884339561776	5.141773575853826	8.687250303977766	3.282132724550651
10	41.32246871303672	31.988386808605675	14.192142898500377	12.497001579857233
11	33.38937277186365	25.45958543222743	25.19469052168375	15.956351274225167
12	29.92278551163387	23.11533784957443	28.041845537937256	18.920031100854445
13	24.025927028793102	29.0450755601876	29.488428166124887	17.440569244894412
14	20.763749307261552	29.94418802782534	30.39074584150144	18.901316823411666
15	20.012489970801592	24.33217945854736	37.141533702242405	18.513796868408644
16	23.57326898103344	22.99064492915457	34.73928219889658	18.696803890915405
17	22.786545571850418	23.334739488986493	29.08291769026527	24.79579724889782
18	24.842014276615632	23.16496687262711	30.919088149416446	21.07393070134081
19	26.93749638121707	26.22345881204662	26.26822832659206	20.570816480144256
20	28.60647906895953	25.49815131889129	25.83190649892057	20.063463113228615
21	25.911933298593016	27.072942256631677	27.682138514603338	19.332985930171965
22	25.349264249733245	23.625689636632835	27.481554546432086	23.543491567201833
23	22.986715964829564	27.8467414410614	27.122571279684358	22.043971314424677
24	22.70910361712863	25.832009892718595	31.7806686683706	19.67821782178218
25	23.129192618509972	25.810297195133046	29.554806984457844	21.505703201899138
26	21.543648725774833	28.225059348040066	27.832162915539676	22.399129010645424
27	21.22984854876465	26.65078537928981	28.995136355740836	23.124229716204702
28	20.136355740837242	27.965127339801647	30.777025070928143	21.121491848432964
29	21.18373491484487	25.927855943489085	30.29531336592306	22.593095775742988
30	22.781686063343177	26.021427330702995	29.99236953770565	21.204517068248176
31	25.762529260444843	26.163180227797216	26.859847638899225	21.21444287285871
32	26.17227888202354	25.851964895737694	27.06084518226259	20.91491103997618
33	24.968154710207862	26.68976484114577	25.993717792831916	22.348362655814455
34	21.310909286417363	27.73021663068562	29.043111078025095	21.915763004871916
35	20.87582818432219	28.163643432012375	28.69022804536093	22.27030033830451
36	24.624370331770017	28.914385799482208	25.492361266201808	20.968882602545968
37	22.19430589675509	29.391341389778074	26.124717734931384	22.289634978535446
38	22.38175885257699	29.65964829565663	25.872126686352846	22.086466165413533
39	22.46654176695865	28.046084683656336	25.35288303266417	24.134490516720845
40	24.076693383624075	25.99785354475297	26.409154073302066	23.516298998320885
41	20.19270536076164	25.601545116917706	27.309300478920072	26.896449043400583
42	22.354669677494066	26.984230377925012	26.006435229989165	24.65466471459176
43	22.00044252545555	25.995165306004285	27.560237226730194	24.44415494180997
44	22.249518184901195	27.076871220956683	27.681518151815183	22.99209244232694
45	21.48399050431359	27.858424940238386	26.515442897673225	24.1421416577748
46	23.11761251313101	27.25563909774436	26.895725286814397	22.73102310231023
47	21.98493345575159	26.88693681398215	28.092818680364278	23.03531104990198
48	22.301628659106512	26.25975003515389	28.89225952670455	22.546361779035045
49	23.17489267723765	25.331066941280593	28.69818936780896	22.795851013672795
50	21.164813849806034	27.503991000603822	28.771288783013638	22.559906366576506
51	21.203689917863965	27.591875728926276	27.36740779341092	23.837026559798836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	802.0
1	1075.5
2	1349.0
3	5647.0
4	9945.0
5	6726.5
6	3508.0
7	3611.0
8	3714.0
9	3885.0
10	4056.0
11	4140.5
12	4225.0
13	4324.5
14	4424.0
15	4241.0
16	4058.0
17	4022.0
18	3986.0
19	3839.5
20	3693.0
21	4031.5
22	4370.0
23	4646.5
24	4923.0
25	5492.0
26	7232.0
27	8403.0
28	10422.5
29	12442.0
30	14168.5
31	15895.0
32	17836.5
33	19778.0
34	23318.0
35	26858.0
36	28153.0
37	29448.0
38	33598.5
39	37749.0
40	43221.5
41	48694.0
42	54347.0
43	60000.0
44	62936.5
45	65873.0
46	69434.0
47	72995.0
48	76212.0
49	79429.0
50	77672.5
51	75916.0
52	70554.0
53	65192.0
54	60580.0
55	55968.0
56	53140.0
57	50312.0
58	46015.5
59	41719.0
60	39069.0
61	36419.0
62	33317.0
63	30215.0
64	26772.0
65	23329.0
66	20224.0
67	17119.0
68	14488.0
69	11857.0
70	9828.0
71	7799.0
72	6584.5
73	5370.0
74	4842.5
75	3452.0
76	2589.0
77	1891.0
78	1193.0
79	955.5
80	718.0
81	512.5
82	307.0
83	199.5
84	92.0
85	66.5
86	41.0
87	27.5
88	14.0
89	9.5
90	5.0
91	4.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	967176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.23468813734474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54688257645503	23.707608233259418
2	7.443453826860792	4.798748255471659
3	3.3792224788490324	3.267845482572108
4	2.1567322914583054	2.7808637124359747
5	1.5590132339213614	2.5127152698724182
6	1.2542333945891602	2.4257893395614896
7	1.0348297296297762	2.3350189526908025
8	0.901787908563272	2.325508159885234
9	0.7362984852464979	2.136091684312817
>10	7.871948611811484	49.72527721202472
>50	0.09056786221934982	1.808663874849517
>100	0.023712253006690127	1.365120513993674
>500	9.880105419395567E-4	0.17474052150445707
>1k	0.0	0.0
>5k	3.2933684731318555E-4	0.6360087875657364
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5991	0.6194322439762774	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04342539517109606	0.0
2	0.0	0.0	0.0	0.14247665368040563	0.0
3	0.0	0.0	0.0	0.2306715633969412	0.0
4	0.0	0.0	0.0	0.410680165760937	0.0
5	0.0	0.0	0.0	0.6886026948559517	0.0
6	0.0	0.0	0.0	1.3335732069447546	0.0
7	0.0	0.0	0.0	1.6436512072259857	0.0
8	0.0	0.0	0.0	2.424170988527424	0.0
9	0.0	0.0	0.0	2.8147927574712357	0.0
10	0.0	0.0	0.0	3.3367246499086	0.0
11	0.0	0.0	0.0	3.7396502808175556	0.0
12	0.0	0.0	0.0	4.074129217433022	0.0
13	0.0	0.0	0.0	4.25000206787596	0.0
14	0.0	0.0	0.0	4.3462616938385565	0.0
15	0.0	0.0	0.0	4.435283753939304	0.0
16	0.0	0.0	0.0	4.586342092855902	0.0
17	0.0	0.0	0.0	4.755390952629098	0.0
18	0.0	0.0	0.0	4.9847183966516955	0.0
19	0.0	0.0	0.0	5.102070357411681	0.0
20	0.0	0.0	0.0	5.232966905713128	0.0
21	0.0	0.0	0.0	5.384749001215911	0.0
22	0.0	0.0	0.0	5.5522469540187105	0.0
23	0.0	0.0	0.0	5.746627294308378	0.0
24	0.0	0.0	0.0	5.906577499855248	0.0
25	0.0	0.0	0.0	6.043884463634333	0.0
26	0.0	0.0	0.0	6.182121971595656	0.0
27	0.0	0.0	0.0	6.321806992729348	0.0
28	0.0	0.0	0.0	6.46469722160186	0.0
29	0.0	0.0	0.0	6.618650586863198	0.0
30	0.0	0.0	0.0	6.816546316285764	0.0
31	0.0	0.0	1.0339379802641919E-4	6.974221858276053	0.0
32	0.0	0.0	1.0339379802641919E-4	7.1600205133295285	0.0
33	0.0	0.0	1.0339379802641919E-4	7.338478208723128	0.0
34	0.0	0.0	1.0339379802641919E-4	7.506493130516059	0.0
35	0.0	0.0	1.0339379802641919E-4	7.69415287393401	0.0
36	0.0	0.0	1.0339379802641919E-4	7.861444039140756	0.0
37	0.0	0.0	1.0339379802641919E-4	8.036489739199483	0.0
38	0.0	0.0	1.0339379802641919E-4	8.256201560005625	0.0
39	0.0	0.0	1.0339379802641919E-4	8.587888864074378	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCT	20	7.032731E-4	45.0	36
CGTTTTT	3495	0.0	40.236053	1
CGCAGTA	85	0.0	39.705883	37
GCGATTT	160	0.0	39.375	9
CATACGA	150	0.0	39.0	18
GCCGATG	170	0.0	38.382355	9
ACGGGAT	360	0.0	38.125	5
GGCGATA	455	0.0	37.582417	8
CACGACC	780	0.0	37.5	27
TAACGCC	30	1.1397882E-4	37.499996	12
GACACGA	785	0.0	37.261147	25
AACACGT	685	0.0	37.116787	41
AAGGGAT	1255	0.0	37.111553	5
GGCGATT	540	0.0	37.083336	8
GCGTATG	85	0.0	37.058826	1
ACACGAC	790	0.0	37.02532	26
AGGGCGA	1150	0.0	36.978264	6
GCGGGAT	445	0.0	36.91011	5
ACGACCA	800	0.0	36.5625	28
ATTGGGA	870	0.0	36.465515	4
>>END_MODULE