FastQCFastQC Report
Thu 26 May 2016
SRR1547121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547121_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1444144
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70780.49011732901982075No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTA207.0338894E-445.018
GCCGTAT207.0338894E-445.041
TCTAGCG650.041.538461
CGGGATA2000.039.3756
CGTTTTT50350.039.0566061
GGGCGAT33750.037.866677
AGGGCGA17250.037.6956526
TAGGGTC7350.037.653065
CCCGTAG301.14006696E-437.49999635
AGGGTAC7350.037.346946
CACGTGA6500.036.69230743
GCGGGAT4750.036.4736865
CACGACC6850.036.45985427
GGTACCT7100.036.4436658
CACAACG3150.036.4285712
AAGGGCG6800.036.397065
ACGACCA6900.036.19565228
GTCGTAT250.00210728736.019
TATTACG504.8805305E-836.01
ACTCGCT250.00210728736.020
Produced by FastQC (version 0.11.5)