##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547121_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1444144
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.461474755980014	31.0	31.0	33.0	30.0	34.0
2	31.839093608393622	31.0	31.0	34.0	30.0	34.0
3	31.96537949124187	33.0	31.0	34.0	30.0	34.0
4	35.72022665329773	37.0	35.0	37.0	33.0	37.0
5	35.65803756412103	37.0	35.0	37.0	33.0	37.0
6	35.71158762561074	37.0	35.0	37.0	33.0	37.0
7	36.054274365991205	37.0	35.0	37.0	35.0	37.0
8	34.73779761575023	37.0	35.0	37.0	33.0	37.0
9	37.04310096500072	39.0	37.0	39.0	34.0	39.0
10	36.81021006215447	39.0	37.0	39.0	32.0	39.0
11	36.83249869819076	39.0	37.0	39.0	33.0	39.0
12	36.59701871835496	39.0	35.0	39.0	32.0	39.0
13	36.53496396481237	39.0	35.0	39.0	32.0	39.0
14	37.63806725645088	40.0	36.0	41.0	33.0	41.0
15	37.78788888088722	40.0	36.0	41.0	33.0	41.0
16	37.81501567710699	40.0	36.0	41.0	33.0	41.0
17	37.723983203891024	40.0	36.0	41.0	33.0	41.0
18	37.6670553629001	39.0	36.0	41.0	33.0	41.0
19	37.615142949733546	39.0	36.0	41.0	33.0	41.0
20	37.50598416778382	39.0	35.0	41.0	32.0	41.0
21	37.411740103479985	39.0	35.0	41.0	32.0	41.0
22	37.323185222526284	39.0	35.0	41.0	32.0	41.0
23	37.24661391107812	39.0	35.0	41.0	32.0	41.0
24	37.12085290663535	39.0	35.0	41.0	32.0	41.0
25	37.01123225938687	39.0	35.0	41.0	32.0	41.0
26	36.926284359454456	39.0	35.0	41.0	31.0	41.0
27	36.82601111800485	39.0	35.0	41.0	31.0	41.0
28	36.853838675367555	39.0	35.0	41.0	31.0	41.0
29	36.81777578967194	39.0	35.0	41.0	31.0	41.0
30	36.7652976434483	39.0	35.0	41.0	31.0	41.0
31	36.68096879535559	39.0	35.0	41.0	31.0	41.0
32	36.619256805415525	39.0	35.0	41.0	31.0	41.0
33	36.521509627848744	39.0	35.0	41.0	30.0	41.0
34	36.37497922644833	39.0	35.0	41.0	30.0	41.0
35	36.26892193576264	39.0	35.0	41.0	30.0	41.0
36	36.1449903887701	39.0	35.0	41.0	30.0	41.0
37	36.120189537885416	39.0	35.0	41.0	30.0	41.0
38	36.023823109052834	38.0	35.0	41.0	30.0	41.0
39	35.946509489358405	38.0	35.0	41.0	29.0	41.0
40	35.8084602366523	38.0	35.0	41.0	29.0	41.0
41	35.730623815907556	38.0	35.0	40.0	29.0	41.0
42	35.671683710211724	38.0	35.0	40.0	28.0	41.0
43	35.59902890570469	38.0	35.0	40.0	28.0	41.0
44	35.477811769463436	38.0	35.0	40.0	28.0	41.0
45	35.34865151951606	38.0	34.0	40.0	27.0	41.0
46	35.27185862351677	38.0	34.0	40.0	27.0	41.0
47	35.2037338381768	37.0	34.0	40.0	27.0	41.0
48	35.13297358158189	37.0	34.0	40.0	27.0	41.0
49	35.0677993330305	37.0	34.0	40.0	27.0	41.0
50	34.95763026401799	37.0	34.0	40.0	27.0	41.0
51	34.605870328720684	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	2.0
12	5.0
13	12.0
14	15.0
15	39.0
16	106.0
17	228.0
18	494.0
19	903.0
20	1699.0
21	2789.0
22	4015.0
23	6270.0
24	9149.0
25	12962.0
26	17343.0
27	21122.0
28	23748.0
29	26607.0
30	31560.0
31	38306.0
32	48058.0
33	62809.0
34	101675.0
35	149262.0
36	115412.0
37	152631.0
38	223947.0
39	392726.0
40	246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.01260539115213	24.878751703431238	27.45294098095481	13.655701924461827
2	31.844469803565296	25.662399317519586	29.082072147929843	13.411058730985276
3	29.782625555346282	25.763220288281502	30.710718598699298	13.74343555767292
4	25.89540932206207	28.628377779501214	30.052612481857764	15.423600416578957
5	23.191593082130314	32.251769906602114	29.31868290142811	15.237954109839464
6	22.106451988167386	40.60682314229052	26.635017006614298	10.651707862927797
7	88.49256029869598	3.45083315791223	6.336694955627693	1.719911587764101
8	86.26002670093841	5.641473426472706	5.982159673827541	2.116340198761342
9	83.0357637465516	4.697730974196479	9.05242136518242	3.214083914069511
10	38.797793017870795	24.994252650705192	23.13778958331025	13.070164748113761
11	34.671888675921515	25.38708051274665	22.998260561273668	16.942770250058167
12	29.565334204899234	23.394896907787587	28.19074829102915	18.849020596284028
13	23.850807121727474	28.38449628291915	29.234134546139444	18.530562049213927
14	20.990981508769206	29.928386642883257	29.87340597613534	19.207225872212188
15	20.537494875857256	24.265308722675854	36.38529121749632	18.811905183970573
16	23.114730940958797	22.676755226625602	34.6015355809393	19.606978251476306
17	23.41296989773873	22.73471343577926	28.642780775324344	25.20953589115767
18	24.72260384006027	23.501257492327635	30.967064226282144	20.80907444132995
19	27.162249747947577	25.069106681882143	26.968917227090927	20.79972634307936
20	28.794081476639448	25.042724271263804	25.957383751204866	20.20581050089188
21	25.967909017383306	26.234572175627914	28.200719595829778	19.596799211158995
22	25.390888997219115	23.302939319070674	28.12205708018037	23.184114603529842
23	23.24331922578358	26.99003700462004	27.4396459078873	22.32699786170908
24	22.92444520768012	25.036492205763416	32.045834764400226	19.99322782215624
25	22.746762095746682	26.09732824427481	29.465136440687356	21.690773219291152
26	21.427987790691233	28.190817536201372	27.844176204035055	22.537018469072336
27	21.694927929624747	27.230733223279675	28.739654771269347	22.33468407582623
28	20.526969609678815	27.225262854673772	30.9534229273535	21.294344608293912
29	21.348494332975104	25.63297011932328	30.72048216798325	22.298053379718365
30	22.54747449007855	26.980411925680542	29.895010469870044	20.577103114370864
31	25.844029404269936	26.41467886858928	26.747194185621375	20.994097541519405
32	25.966731849455453	25.777207813071275	27.104568519482818	21.15149181799045
33	25.406815386831227	25.723542804595663	26.664723185499504	22.2049186230736
34	22.006877430505543	26.67774127787811	29.005348497102784	22.310032794513564
35	22.828471398974063	26.776415648301	29.09072779445817	21.304385158266765
36	25.724789217695744	26.85971759048959	26.571865409543648	20.84362778227102
37	22.879851316766196	28.28824549352419	27.364514896021447	21.467388293688163
38	23.586221318649663	28.349181245083592	26.716795555013906	21.34780188125284
39	22.89584695155054	27.26494033835961	27.0459178586069	22.793294851482955
40	24.345425386942022	25.35093453284437	27.825272272017195	22.478367808196413
41	21.50304955738486	25.382164105518562	28.035569860069355	25.079216477027224
42	22.7572181167529	26.540843572386137	26.6394486976368	24.062489613224166
43	22.193839395517344	25.32406740602045	28.00766405566204	24.474429142800165
44	22.038453229040872	26.402976434482987	28.294893030057942	23.263677306418195
45	21.206680220255045	28.00655613290641	27.197495540610912	23.589268106227635
46	23.30079271873165	27.36209131499352	27.597455655391705	21.739660310883124
47	22.478298563024186	26.517369459001316	28.554631671079893	22.449700306894606
48	22.76289622087548	25.84520657219779	29.28883823219845	22.10305897472828
49	22.75050133504692	25.32261325740369	29.24258245715109	22.6843029503983
50	21.720964114381946	27.958430738208932	28.68287373004354	21.63773141736558
51	21.52887800662538	27.63380937081067	27.49559600704639	23.341716615517566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	945.0
1	1520.5
2	2096.0
3	7481.0
4	12866.0
5	9036.0
6	5206.0
7	5286.5
8	5367.0
9	5662.0
10	5957.0
11	6147.0
12	6337.0
13	6455.0
14	6573.0
15	6354.0
16	6135.0
17	5971.5
18	5808.0
19	5795.0
20	5782.0
21	5920.5
22	6059.0
23	7190.0
24	8321.0
25	8838.5
26	11411.0
27	13466.0
28	15019.0
29	16572.0
30	19874.0
31	23176.0
32	26856.5
33	30537.0
34	35395.0
35	40253.0
36	43336.0
37	46419.0
38	51814.0
39	57209.0
40	66360.0
41	75511.0
42	84198.5
43	92886.0
44	97301.5
45	101717.0
46	105850.5
47	109984.0
48	113310.0
49	116636.0
50	111676.5
51	106717.0
52	100139.5
53	93562.0
54	86620.0
55	79678.0
56	75663.5
57	71649.0
58	67510.5
59	63372.0
60	60004.5
61	56637.0
62	51979.5
63	47322.0
64	42203.0
65	37084.0
66	31239.5
67	25395.0
68	21644.5
69	17894.0
70	15315.0
71	12736.0
72	10691.0
73	8646.0
74	7143.5
75	4624.0
76	3607.0
77	2643.5
78	1680.0
79	1195.0
80	710.0
81	545.0
82	380.0
83	248.5
84	117.0
85	97.5
86	78.0
87	61.0
88	44.0
89	27.0
90	10.0
91	7.5
92	5.0
93	5.0
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1444144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.658149165774407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.01361754240976	19.503687375332756
2	6.184017261722509	3.1734087468999985
3	2.577496366285338	1.9840135872117213
4	1.5718456936472465	1.6132260509272474
5	1.2137916500019426	1.5571823605960644
6	0.9812208017258585	1.5105785817145696
7	0.8264295723379748	1.4843257269438435
8	0.6986777826574985	1.4341423012990886
9	0.6251000140338205	1.4435018463246696
>10	8.448760550063938	50.62557035918368
>50	0.8148985698797052	12.994511152081737
>100	0.04109019431452331	1.7346769410998553
>500	0.0027763644727453354	0.4369639167680929
>1k	0.0	0.0
>5k	2.7763644727453354E-4	0.504211053616655
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7078	0.49011732901982075	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0773551667977708E-4	0.0	0.0	0.039469748169157645	0.0
2	2.0773551667977708E-4	0.0	0.0	0.14126015134224842	0.0
3	2.0773551667977708E-4	0.0	0.0	0.22290020939740082	0.0
4	2.0773551667977708E-4	0.0	0.0	0.39365880410817755	0.0
5	2.0773551667977708E-4	0.0	0.0	0.6663462923364983	0.0
6	2.0773551667977708E-4	0.0	0.0	1.1997418539979392	0.0
7	2.0773551667977708E-4	0.0	0.0	1.437114304390698	0.0
8	2.0773551667977708E-4	0.0	0.0	1.964208555379519	0.0
9	2.0773551667977708E-4	0.0	0.0	2.229902281212954	0.0
10	2.0773551667977708E-4	0.0	0.0	2.6621306462513434	0.0
11	2.0773551667977708E-4	0.0	0.0	3.010433862551103	0.0
12	2.0773551667977708E-4	0.0	0.0	3.287830022490832	0.0
13	2.0773551667977708E-4	0.0	0.0	3.438992233461483	0.0
14	2.0773551667977708E-4	0.0	0.0	3.5078219346547157	0.0
15	2.0773551667977708E-4	0.0	0.0	3.5864844503041247	0.0
16	2.0773551667977708E-4	0.0	0.0	3.7404857133360663	0.0
17	2.0773551667977708E-4	0.0	0.0	3.899888099801682	0.0
18	2.0773551667977708E-4	0.0	0.0	4.1159330371486496	0.0
19	2.0773551667977708E-4	0.0	0.0	4.227417764433464	0.0
20	2.0773551667977708E-4	0.0	0.0	4.337794568962652	0.0
21	2.0773551667977708E-4	0.0	0.0	4.4853560309775204	0.0
22	2.0773551667977708E-4	0.0	0.0	4.64385823020419	0.0
23	2.0773551667977708E-4	0.0	0.0	4.8175943673207104	0.0
24	2.0773551667977708E-4	0.0	0.0	4.960100931763037	0.0
25	2.0773551667977708E-4	0.0	0.0	5.088412235898913	0.0
26	2.0773551667977708E-4	0.0	0.0	5.209245061434316	0.0
27	2.0773551667977708E-4	0.0	0.0	5.335686745920074	0.0
28	2.0773551667977708E-4	0.0	0.0	5.4624746562669655	0.0
29	2.0773551667977708E-4	0.0	0.0	5.60054952968679	0.0
30	2.0773551667977708E-4	0.0	0.0	5.779340564375851	0.0
31	2.0773551667977708E-4	0.0	0.0	5.922193354679312	0.0
32	2.0773551667977708E-4	0.0	0.0	6.081803476661607	0.0
33	2.0773551667977708E-4	0.0	0.0	6.228810977298663	0.0
34	2.0773551667977708E-4	0.0	0.0	6.3842663889473625	0.0
35	2.0773551667977708E-4	0.0	0.0	6.553293854352474	0.0
36	2.0773551667977708E-4	0.0	0.0	6.703486632911954	0.0
37	2.0773551667977708E-4	0.0	0.0	6.8609501545552245	0.0
38	2.0773551667977708E-4	0.0	0.0	7.041541563722177	0.0
39	2.0773551667977708E-4	0.0	0.0	7.2879158865043925	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGTA	20	7.0338894E-4	45.0	18
GCCGTAT	20	7.0338894E-4	45.0	41
TCTAGCG	65	0.0	41.53846	1
CGGGATA	200	0.0	39.375	6
CGTTTTT	5035	0.0	39.056606	1
GGGCGAT	3375	0.0	37.86667	7
AGGGCGA	1725	0.0	37.695652	6
TAGGGTC	735	0.0	37.65306	5
CCCGTAG	30	1.14006696E-4	37.499996	35
AGGGTAC	735	0.0	37.34694	6
CACGTGA	650	0.0	36.692307	43
GCGGGAT	475	0.0	36.473686	5
CACGACC	685	0.0	36.459854	27
GGTACCT	710	0.0	36.443665	8
CACAACG	315	0.0	36.42857	12
AAGGGCG	680	0.0	36.39706	5
ACGACCA	690	0.0	36.195652	28
GTCGTAT	25	0.002107287	36.0	19
TATTACG	50	4.8805305E-8	36.0	1
ACTCGCT	25	0.002107287	36.0	20
>>END_MODULE