FastQCFastQC Report
Thu 26 May 2016
SRR1547120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547120_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1090652
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107250.9833567444060984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65050.041.6794781
ATAACGC556.184564E-1140.90909211
ATATACG451.9281288E-840.01
AGGGCGT1050.038.571436
TACGGGA3050.038.3606574
TACGGGT1350.038.3333364
AAGGGCG5550.038.108115
TCACGAC659.094947E-1238.0769225
GTCGTTT659.094947E-1238.076929
GCGCGAC3050.037.622959
ACGGGAC2100.037.5000045
TAACGCC601.5643309E-1037.49999612
CGGTCTA601.5643309E-1037.49999631
AGGGCGA13750.037.3090936
CGTTAGG1450.037.2413832
GGGCGAT26100.037.0689667
TATGGGT4250.036.529414
CACGACC6850.036.45985427
ACGACCA6950.036.25899528
AGGGATT7900.036.1708876
Produced by FastQC (version 0.11.5)