FastQCFastQC Report
Thu 26 May 2016
SRR1547119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547119_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1072798
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52240.48695094509870446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG351.211829E-745.01
CTCGCTA253.8906306E-545.045
GTTCACG207.033077E-445.01
CGGTCTA207.033077E-445.031
ATACGAC351.211829E-745.014
TCGACAG207.033077E-445.01
AATACCG207.033077E-445.035
TCGAAAG406.8139343E-945.01
TCGATAG603.6379788E-1241.2499961
CGTTTTT40450.041.0506821
CGTTCGA501.0822987E-940.514
TGGGCGA7050.040.2127656
AACGGGA2700.040.0000044
TAACGGG2350.039.2553183
TACGGGA2300.039.1304324
GGCGATA5300.039.0566028
TATGGGC4800.038.9062464
TAGGGCG4650.038.7096755
ACTGACG356.2478994E-638.5714261
CGCATCG356.2478994E-638.57142621
Produced by FastQC (version 0.11.5)