FastQCFastQC Report
Thu 26 May 2016
SRR1547118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547118_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences562435
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29660.5273498270911305No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14340.2549627957008365No Hit
GAGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT6670.11859148168232773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.0302107E-445.01
ATTAGCG207.0302107E-445.01
GTCGAAT207.0302107E-445.013
ATAGGAC207.0302107E-445.026
TGAAGCG207.0302107E-445.01
GGCGTAT253.888256E-545.08
TAGCGAA207.0302107E-445.029
TAACGCG207.0302107E-445.01
TGTTACG453.8380676E-1045.01
CGAATAT351.2104465E-745.014
CCGACAG207.0302107E-445.012
TGTAGCG453.8380676E-1045.01
GTCCCGT207.0302107E-445.027
GCTACGA351.2104465E-745.010
CTTTACG253.888256E-545.01
CGCGTAA351.2104465E-745.031
CGACTAA351.2104465E-745.030
TAATGCG207.0302107E-445.01
TCGAATC253.888256E-545.014
GCGTAAC351.2104465E-745.032